Inhibition of aflatoxin B₁production ofAspergillus flavus, isolated from soybean seeds by certain natural plant products

Abstract

Aims: The inhibitory effect of cowdung fumes, Captan, leaf powder of Withania somnifera, Hyptis suaveolens, Eucalyptus citriodora, peel powder of Citrus sinensis, Citrus medica and Punica granatum, neem cake and pongamia cake and spore suspension of Trichoderma harzianum and Aspergillus niger on aflatoxin B1 production by toxigenic strain of Aspergillus flavus isolated from soybean seeds was investigated. Methods and Results: Soybean seed was treated with different natural products and fungicide captan and was inoculated with toxigenic strain of A. flavus and incubated for different periods. The results showed that all the treatments were effective in controlling aflatoxin B1 production. Captan, neem cake, spore suspension of T. harzianum, A. niger and combination of both reduced the level of aflatoxin B1 to a great extent. Leaf powder of W. somnifera, H. suaveolens, peel powder of C. sinensis, C. medica and pongamia cake also controlled the aflatoxin B1 production. Conclusions: All the natural product treatments applied were significantly effective in inhibiting aflatoxin B1 production on soybean seeds by A. flavus. Significance and Impact of the Study: These natural plant products may successfully replace chemical fungicides and provide an alternative method to protect soybean and other agricultural commodities from aflatoxin B1 production by A. flavus.

Interaction Between Certain Moulds and Aflatoxin B1 Producer Aspergillus Flavus NRRL 3251

“…The three strains were aggressive inhibitors of A. flavus growth, and macroscopic observations of mixed cultures showed highly predominant sporulating of A. niger, whereas AFB 1 was not detected. One strain led to a 90 % inhibition (C mean =1.9 µg mL -1 ) and another in 80 % inhibition (C mean =3.7 µg mL -1 ), primarily due to the action of gluconic acid, which is produced by A. niger (21)(22)(23).…”

Anti-aflatoxigenic Effect of Some Lactobacillus Species on Aspergillus flavus by Using Real – Time - q PCR

“…Among all aflatoxins, Aflatoxin B 1 is the most abundant aflatoxin and is considered to be the most toxic. Colonization of food with aflatoxigenic A. flavus is of importance because of its potential to produce aflatoxins which are potent toxic, carcinogenic, mutagenic, immunosuppressive agents (Calvo et al, 2002 andKrishnamurthy &Shashikala, 2006). Aflatoxin not only gives rise to cases of poisoning but is also associated with liver cancer and growth retardation among children (Gong et al, 2004 andTsugane, 2004).…”

Changes in the microbiological and physicochemical quality during storage of osmotically dehydrated strawberry jam stabilized with plant extracts

“…Email: mdmcamvi@tal.upv.es Pomegranate (Punica granatum L.) is a very rich source of anthocyanins, ellagitannins, and other phenolic compounds with proven antioxidant activity (Madrigal-Carballo, Rodriguez, Krueger, Dreher, & Reed, 2009) and antimicrobial effects (Voravuthikunchai, Lortheeranuwat, & Jeeju, 2004). Pomegranate is effective against Aspergillus flavus (Krishnamurthy & Shashikala, 2006). Lemon (Citrus limon) has antimicrobial, antifungal (Viuda-Martos, Ruiz-Navajas, Fernandez-Lopez, & Perez-Alvarez, 2008), and antioxidant activities (Xu, Liu, Chen, Ye, Ma, & Shi, 2008).…”