Inhibition of Adipocyte Differentiation by Anthocyanins Isolated from the Fruit of<i>Vitis coignetiae</i>Pulliat is Associated with the Activation of AMPK Signaling Pathway

Han, Min; Kim, Hong Jae; Jeong, Jin‐Woo; Park, Cheol; Kim, Byung Woo; Choi, Yung Hyun

doi:10.5487/tr.2018.34.1.013

Cited by 57 publications

(36 citation statements)

References 37 publications

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“…Activation of AMPK signalling pathway acts as a sensor of cellular energy status, and inhibition of preadipocyte differentiation and adipogenesis through increased phosphorylation of its substrate, acetyl-CoA carboxylase (ACC) [22]. Interestingly, a widely prescribed drug for type 2 diabetes-metformin inhibits hepatic gluconeogenic programme by activation of AMPK pathway [23] long-term usage of which may, therefore, have negative consequences on normal adipose physiology.…”

Section: Gene Expression Analysismentioning

confidence: 99%

Transcriptome profiling reveals association of peripheral adipose tissue pathology with type-2 diabetes in Asian Indians

et al. 2019

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Type 2 diabetes (T2D) is a complex disease with an elusive link between its molecular aetiology and clinical presentation. Although, the role of visceral adipose tissue in insulin-resistance and T2D is known, limited information is available on the role of peripheral-subcutaneous adipose tissue especially in Asian Indians. In this microarray-based study of diabetic and normal glucose tolerant Asian Indians, we generated the transcriptome of their thigh adipose tissue and analyzed differentially expressed genes (DEGs) using weighted gene co-expression network analysis; further we identified perturbed pathways implicated by these DEGs in relevant co-expression modules. We also attempted to link these pathways with known aspects of T2D pathophysiology in terms of their association with some of their intermediate traits, namely; adipocyte size, HOMA-B, HOMA-R, Hb1Ac, insulin, glucose-level, TNF-α, IL-6, VLDLs, LDLs, HDLs, and NEFAs. It was observed that several modules of co-expressed genes show an association with diabetes and some of its intermediate phenotypic traits mentioned above. Therefore, these findings suggest a role of peripheral subcutaneous adipose tissue in the pathophsiology of T2D in Asian Indians. Additionally, our study indicated that the peripheral subcutaneous adipose tissue in diabetics shows pathologic changes characterized by adipocyte hypertrophy and up-regulation of inflammation-related pathways.

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Section: Gene Expression Analysismentioning

confidence: 99%

Transcriptome profiling reveals association of peripheral adipose tissue pathology with type-2 diabetes in Asian Indians

et al. 2019

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“…Our results in 3T3-L1 are in accordance with other authors who have used these cells. Treatment with 140 µg/mL GSPE at the onset of differentiation reduced adipogenesis markers [ 7 ], while an inhibition of lipid content and adipogenic transcription factors was observed with a concentration of 100 µg/mL of anthocyanins extracted from Vitis coignetiae [ 8 ]. Unexpectedly, we obtained no differences in the lipid content of cells with CLA treatment, while other authors showed that 10 mg/L of CLA isomers, consisting principally of 41% c9, t11 and 44% t10, c12, inhibited proliferation of 3T3-L1 preadipocytes while promoting lipogenesis [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…Although adipocytes are the principal cells in WAT, it also includes a heterogeneous cell population called the stromal vascular fraction (SVF) [ 6 ]. Adipocytes have been extensively studied in vitro in the murine 3T3-L1 cell line, which is a good model to study the effects of particular compounds on adipocyte differentiation [ 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

A Mix of Natural Bioactive Compounds Reduces Fat Accumulation and Modulates Gene Expression in the Adipose Tissue of Obese Rats Fed a Cafeteria Diet

et al. 2020

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Scientists are focusing on bioactive ingredients to counteract obesity. We evaluated whether a mix containing grape seed proanthocyanidin extract (GSPE), anthocyanins, conjugated linoleic acid (CLA), and chicken feet hydrolysate (CFH) could reduce body fat mass and also determined which mechanisms in the white adipose tissue (WAT) and the brown adipose tissue (BAT) were affected by the treatment. The mix or vehicle (VH) were administered for three weeks to obese rats fed a cafeteria (CAF) diet. Biometric measures, indirect calorimetry, and gene expression in WAT and BAT were analyzed as was the histology of the inguinal WAT (IWAT). The individual compounds were also tested in the 3T3-L1 cell line. The mix treatment resulted in a significant 15% reduction in fat (25.01 ± 0.91 g) compared to VH treatment (21.19 ± 1.59 g), and the calorimetry results indicated a significant increase in energy expenditure and fat oxidation. We observed a significant downregulation of Fasn mRNA and an upregulation of Atgl and Hsl mRNA in adipose depots in the group treated with the mix. The IWAT showed a tendency of reduction in the number of adipocytes, although no differences in the total adipocyte area were found. GSPE and anthocyanins modulated the lipid content and downregulated the gene and protein levels of Fasn compared to the untreated group in 3T3-L1 cells. In conclusion, this mix is a promising treatment against obesity, reducing the WAT of obese rats fed a CAF diet, increasing energy expenditure and fat oxidation, and modifying the expression of genes involved in lipid metabolism of the adipose tissue.

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“…Conventionally, AMPK is activated by an increase of AMP/ATP ratio via phosphorylation of its catalytic α subunit (AMPKα) at Thr172 in the case of environmental and cellular stress, such as heat shock, hypoxia, ischemia, exercise, and long‐term starvation, resulting in enhanced glucose uptake and fatty acid oxidation to re‐establish cellular energy homeostasis (Carling, ). In addition, it has been shown that AMPK plays a vital role in cell differentiation, which involves suppressing adipogenic differentiation while promoting osteogenic differentiation (Han et al, ; Qin et al, ). In the present study, berberine markedly increased AMPKα phosphorylation at Thr172 in a concentration‐dependent manner in porcine adipocytes (Fig.…”

Section: Discussionmentioning

confidence: 99%

Berberine Inhibits Adipogenesis in Porcine Adipocytes via AMP‐Activated Protein Kinase‐Dependent and ‐Independent Mechanisms

Yang

Liu

et al. 2019

Lipids

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Excessive adipogenesis in adipocytes results in obesity. Berberine, a natural isoquinoline alkaloid, has antiobesity properties. However, the underlying molecular mechanisms have remained unclear up to now. In this study, porcine adipocytes were cultured and treated with berberine. Cellular lipid content was measured by Oil Red O staining extraction. The role of an adenosine monophosphate‐activated protein kinase (AMPK) signaling pathway was evaluated by the phosphorylation detection of AMPKα protein and knockdown of AMPK alpha1 (Ampka1) gene. Gene expressions were analyzed by Western blot and real‐time reverse transcription‐polymerase chain reaction (RT‐PCR). The results showed that berberine reduced lipid accumulation in porcine adipocytes in a dose‐ and time‐dependent manner and increased phosphorylation of AMPKα. Furthermore, berberine significantly downregulated the mRNA expression of related genes to adipogenesis including peroxisome proliferator activated receptor gamma 2 (Pparg2), CCAAT/enhancer‐binding protein alpha (Cebpa), Cebp beta (Cebpb), sterol regulatory element binding transcription factor 1 (Srebf1), acetyl‐CoA carboxylase‐1 (Acc‐1), fatty acid synthase (Fas), fatty acid binding protein 4 (Fabp4), and stearoyl‐CoA desaturase 1 (Scd1). Knockdown of Ampka1 markedly reversed the inhibitory effect of berberine on lipid accumulation and mRNA expression of the above genes except Cebpb in porcine adipocytes. Meanwhile, the protein expression of these adipogenic genes in response to berberine and Ampka1 knockdown paralleled the alterations of their mRNA level. These results suggest that berberine inhibits adipogenesis in porcine adipocytes via AMPK‐dependent and ‐independent multiple mechanisms, which would provide an important idea for the reduction of porcine body fat, as well as the prevention and treatment of human obesity.

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Inhibition of Adipocyte Differentiation by Anthocyanins Isolated from the Fruit ofVitis coignetiaePulliat is Associated with the Activation of AMPK Signaling Pathway

Cited by 57 publications

References 37 publications

Transcriptome profiling reveals association of peripheral adipose tissue pathology with type-2 diabetes in Asian Indians

Transcriptome profiling reveals association of peripheral adipose tissue pathology with type-2 diabetes in Asian Indians

A Mix of Natural Bioactive Compounds Reduces Fat Accumulation and Modulates Gene Expression in the Adipose Tissue of Obese Rats Fed a Cafeteria Diet

Berberine Inhibits Adipogenesis in Porcine Adipocytes via AMP‐Activated Protein Kinase‐Dependent and ‐Independent Mechanisms

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