Inhibition of Adhesion, Proliferation, and Invasion of Primary Endometriosis and Endometrial Stromal and Ovarian Carcinoma Cells by a Nonhyaluronan Adhesion Barrier Gel

Renner, Stefan P.; Strissel, Pamela L.; Beckmann, Matthias W.; Lermann, Johannes; Burghaus, Stefanie; Hackl, Janina; Fasching, Peter A.; Strick, Reiner

doi:10.1155/2015/450468

Cited by 9 publications

(5 citation statements)

References 59 publications

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“…In addition to the uneven relationship between growth factors and apoptosis, ectopic endometrial cells are very resistant to apoptosis. Altered cells of the peritoneum enable better efficiency of proteins that promote proliferation and reduced function of apoptotic proteins ( Renner et al ., 2015 ). These are just some of the processes that occur during the development of endometriosis.…”

Section: Discussionmentioning

confidence: 99%

Concentration of total proteins in urine as a non-invasive biomarker of endometriosis

Visnic,

Barisic,

Domitrovic

et al. 2023

JBRA

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Objective The disease in which we observe the invasion and growth of endometrial cells on extrauterine tissues and organs with the creation of a chronic inflammatory state is called endometriosis. It causes infertility and is present in more than 30% of patients with endometriosis. Diagnosis and treatment of the disease is most often delayed for about 8 years after the first symptoms were reported. The symptomatology of endometriosis is varied, and there is no non-invasive way of diagnosis, and this is the reason for the delayed start of treatment. The development of endometriosis activates pathological processes such as the invasion and proliferation of endometriotic cells, the formation of adhesions and the activation of the immune system, which result in increased protein expression. The aim of this research is to compare the concentrations of total proteins in the urine of subjects with endometriosis with those of the control group and possibly identify a biomarker for the diagnosis of endometriosis. Methods Prospective urine analysis of 141 patients who were hospitalized and surgically treated at the Clinic for Gynecology and Obstetrics of KBC Rijeka from 08/21/2021 until 07/30/2022. The urine of subjects with endometriosis (N=84) and without endometriosis (n=57) was analyzed. Results Total protein in the urine is increased in the urine of subjects with endometriosis, but the total amount of protein does not correlate with the degree of disease progression. Conclusions An increase in the level of total proteins in urine in subjects with endometriosis is a possible non-invasive diagnostic biomarker. Patients with endometriosis are grouped after the concentration of total proteins greater than 5000 µg/µl.

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Section: Discussionmentioning

confidence: 99%

Concentration of total proteins in urine as a non-invasive biomarker of endometriosis

Visnic,

Barisic,

Domitrovic

et al. 2023

JBRA

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“…After an incubation time of 72 h, cells were fixed in 4% paraformaldehyde. All invaded cells were counted in >20 optical fields (Carl Zeiss AG, Oberkochen, Germany) per well and represented as number of invaded cells per cm 2 according to 26,27 .…”

Section: Methodsmentioning

confidence: 99%

Plasticity of patient-matched normal mammary epithelial cells is dependent on autologous adipose-derived stem cells

Kengelbach‐Weigand

Tasbihi

Strissel

et al. 2019

Sci Rep

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Due to the increasing clinical application of adipose-derived stem cells (ADSC), e.g. lipotransfer for breast reconstruction, this study aimed to gain novel insights regarding ADSC influence on breast tissue remodeling and determine patient-dependent factors affecting lipotransfer as well as begin to address its oncological risks. The ADSC secretome was analyzed from five normal breast reduction patients and contained elevated levels of growth factors, cytokines and proteins mediating invasion. ADSC/ADSC secretomes were tested for their influence on the function of primary mammary epithelial cells, and tumor epithelial cells using cell culture assays. ADSC/ADSC secretomes significantly stimulated proliferation, transmigration and 3D-invasion of primary normal and tumor epithelial cells. IL-6 significantly induced an EMT and invasion. The ADSC secretome significantly upregulated normal epithelial cell gene expression including MMPs and ECM receptors. Our study supports that ADSC and its secretome promote favorable conditions for normal breast tissue remodeling by changing the microenvironment. and may also be important regarding residual breast cancer cells following surgery.

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“…Cell invasion assays were performed according to previous reports [ 26 , 27 ]. Calf skin type I collagen G (Serva Electrophoresis GmbH, Heidelberg, Germany) and rat tail type I collagen R (Biochrom AG, Berlin, Germany) were mixed at a ratio of 1:1 plus 0.1 volume of sodium bicarbonate (23 mg/ml), 0.1 volume of 10 × DMEM, and then the solution was neutralized with sodium hydroxide.…”

Section: Methodsmentioning

confidence: 99%

“…After 72 h all cells were fixed in 4 % paraformaldehyde. All invaded cells were counted in >20 optical fields per well and represented as number of cells per cm 2 according to previous reports [ 26 , 27 ]. Criteria for mesenchymal and amoeboid phenotypes were as previously reported [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells

et al. 2016

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BackgroundThere is a need to establish more cell lines from breast tumors in contrast to immortalized cell lines from metastatic effusions in order to represent the primary tumor and not principally metastatic biology of breast cancer. This investigation describes the simultaneous isolation, characterization, growth and function of primary mammary epithelial cells (MEC), mesenchymal cells (MES) and adipose derived stem cells (ADSC) from four normal breasts, one inflammatory and one triple-negative ductal breast tumors.MethodsA total of 17 cell lines were established and gene expression was analyzed for MEC and MES (n = 42) and ADSC (n = 48) and MUC1, pan-KRT, CD90 and GATA-3 by immunofluorescence. DNA fingerprinting to track cell line identity was performed between original primary tissues and isolates. Functional studies included ADSC differentiation, tumor MES and MEC invasion co-cultured with ADSC-conditioned media (CM) and MES adhesion and growth on 3D-printed scaffolds.ResultsComparative analysis showed higher gene expression of EPCAM, CD49f, CDH1 and KRTs for normal MEC lines; MES lines e.g. Vimentin, CD10, ACTA2 and MMP9; and ADSC lines e.g. CD105, CD90, CDH2 and CDH11. Compared to the mean of all four normal breast cell lines, both breast tumor cell lines demonstrated significantly lower ADSC marker gene expression, but higher expression of mesenchymal and invasion gene markers like SNAI1 and MMP2. When compared with four normal ADSC differentiated lineages, both tumor ADSC showed impaired osteogenic and chondrogenic but enhanced adipogenic differentiation and endothelial-like structures, possibly due to high PDGFRB and CD34. Addressing a functional role for overproduction of adipocytes, we initiated 3D-invasion studies including different cell types from the same patient. CM from ADSC differentiating into adipocytes induced tumor MEC 3D-invasion via EMT and amoeboid phenotypes. Normal MES breast cells adhered and proliferated on 3D-printed scaffolds containing 20 fibers, but not on 2.5D-printed scaffolds with single fiber layers, important for tissue engineering.ConclusionExpression analyses confirmed successful simultaneous cell isolations of three different phenotypes from normal and tumor primary breast tissues. Our cell culture studies support that breast-tumor environment differentially regulates tumor ADSC plasticity as well as cell invasion and demonstrates applications for regenerative medicine.Electronic supplementary materialThe online version of this article (doi:10.1186/s13058-016-0688-2) contains supplementary material, which is available to authorized users.

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Inhibition of Adhesion, Proliferation, and Invasion of Primary Endometriosis and Endometrial Stromal and Ovarian Carcinoma Cells by a Nonhyaluronan Adhesion Barrier Gel

Cited by 9 publications

References 59 publications

Concentration of total proteins in urine as a non-invasive biomarker of endometriosis

Concentration of total proteins in urine as a non-invasive biomarker of endometriosis

Plasticity of patient-matched normal mammary epithelial cells is dependent on autologous adipose-derived stem cells

Selective isolation and characterization of primary cells from normal breast and tumors reveal plasticity of adipose derived stem cells

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