Inhibition of adenovirus replication by CRISPR-Cas9-mediated targeting of the viral E1A gene

Didara, Zrinka; Reithofer, Florian; Zöttl, Karina; Jürets, Alexander; Kiss, Izabella; Witte, Angela; Klein, Reinhard

doi:10.1016/j.omtn.2023.02.033

Cited by 2 publications

(2 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As research and development continue, addressing challenges and ethical considerations while capitalizing on the opportunities will be pivotal in maximizing the benefits of viral CRISPR/Cas9 technology for the betterment of agriculture and society as a whole. [32] Adeno-associated virus (AAV) Single-stranded DNA virus CRISPR/Cas9 system (Y. Wang et al, 2022) [128]…”

Section: Overall Potential To Enhance Crop Resilience Against Biotic ...mentioning

confidence: 99%

Viral delivery of CRISPR/Cas9 genome editing for rapid crop improvement: A promising approach to enhance crop resilience against biotic and abiotic stresses

Patil,

Wagh,

Janvale

et al. 2024

Int. J. Adv. Biochem. Res.

View full text Add to dashboard Cite

Agricultural production faces challenges from disease, pests, and environmental stresses, necessitating enhanced crop cultivars. Traditional transformation techniques are limited, leading to few cultivars for genetic manipulation. To overcome this, we explore viral delivery of CRISPR/Cas9 for precise plant genome modifications. This method utilizes optimized viruses to deliver CRISPR/Cas9 constructs, enabling targeted changes. By spanning the virus host range, traditional transformation challenges are circumvented. We review virus types used for delivery, recent outcomes, and future potential. Successful cases in various crops show improved disease resistance, insect tolerance, and stress resilience. The technology can target multiple traits simultaneously, streamlining crop improvement. Challenges like off-target effects and regulations need addressing for broader adoption. Viral delivery of CRISPR/Cas9 holds promise for accelerating crop breeding, enhancing resilience, and contributing to global food security and sustainable agriculture.

show abstract

Section: Overall Potential To Enhance Crop Resilience Against Biotic ...mentioning

confidence: 99%

Viral delivery of CRISPR/Cas9 genome editing for rapid crop improvement: A promising approach to enhance crop resilience against biotic and abiotic stresses

Patil,

Wagh,

Janvale

et al. 2024

Int. J. Adv. Biochem. Res.

View full text Add to dashboard Cite

show abstract

“…Unlike retrovirus, adenovirus is a non-enveloped virus, it, cannot be integrated into the chromosomes and is only expressed transiently. Thanks to high transduction efficiency and the broadness of different tissue targets as a carrier for gene therapy [ 56 ], it is widely used in genetic modification. First-generation adenovirus vectors fail to replicate in host cells, and transducer cells are cleared due to de novo expression of adenoviral proteins, activating the host immune response.…”

Section: Vectors For Nucleic Acid Drugsmentioning

confidence: 99%