Inhibition of actin polymerization by latrunculin A

Coué, Martine; Brenner, Stephen L.; Spector, Ilan; Korn, Edward D.

doi:10.1016/0014-5793(87)81513-2

Cited by 772 publications

(652 citation statements)

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“…Unlike CB, LatA can bind specifically to G-actin and has no activity to block the barbed end of F-actin [25]. In addition, it was also reported that the LatA effect of cytoskeletal inhibition in mammalian cells was inactivated within 1 h when the cells were placed in fresh medium without LatA [24]. Thus, we consider that this rapid reorganization of cortical actin appears to have contributed to improvement of the blastocyst formation rate of SCNT embryos.…”

Section: Discussionmentioning

confidence: 86%

“…After being washed twice with Hepes-TLP-PVA (Tyrode-lactate-pyruvate-polyvinyl alcohol) and the maturation medium, respectively, COCs with compact cumulus cells and evenly granulated ooplasm were used for the experiments. COCs (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) were cultured in a droplet of maturation medium (200 µl) under paraffin oil (Nacalai Tesque, Kyoto, Japan) in a polystyrene dish (35-mm: Becton Dickinson Labware, Oxnard, CA, USA) and cultured at 38.5 C in an atmosphere of 5% CO 2 in air. The maturation medium was TCM-199 with Earle's salts (Gibco, BRL, Grand Island, NY, USA) supplemented with 0.91 mM sodium pyruvate (Sigma-Aldrich Chemical, St. Louis, MO, USA), 3.05 mM glucose (Wako Pure Chemical Industries, Osaka, Japan), 0.57 mM cysteine hydrochloride hydrate (Sigma), 10 ng/ml epidermal growth factor (Sigma), 10 IU/ml eCG (ASKA Pharmaceutical, Tokyo, Japan), 10 IU/ml hCG (ASKA), 100 µg/ml amikacin sulfate (Meiji Seika, Tokyo, Japan) and 10% (v/v) pig follicular fluid.…”

Section: In Vitro Maturation Of Oocytesmentioning

confidence: 99%

“…However, it has been reported that postactivation treatment with CB had no effect on either blastocyst rate or cell number of SCNT embryos [9,22]. CB possess cytotoxicity [23], and its cytotoxicity might have a detrimental effect on the subsequent development of SCNT embryos.Latrunculin A (LatA), a toxin purified from the Red Sea sponge Latrunculia magnifica, is a member of the cytoskeletal inhibitors, which inhibit the polymerization of actin as well as CB [24]. LatA affects the polymerization of pure actin, a major component of the cytoskeleton, in vitro in a manner consistent with formation of a 1:1 molar complex between LatA and G-actin [26].…”

mentioning

confidence: 99%

“…Latrunculin A (LatA), a toxin purified from the Red Sea sponge Latrunculia magnifica, is a member of the cytoskeletal inhibitors, which inhibit the polymerization of actin as well as CB [24]. LatA affects the polymerization of pure actin, a major component of the cytoskeleton, in vitro in a manner consistent with formation of a 1:1 molar complex between LatA and G-actin [26].…”

mentioning

confidence: 99%

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Effect of Postactivation Treatment with Latrunculin A on In Vitro and In Vivo Development of Cloned Embryos Derived from Kidney Fibroblasts of an Aged Clawn Miniature Boar

Himaki

Mizobe

Tsuda

et al. 2012

Journal of Reproduction and Development

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Abstract. The objective of this study was to examine the effect of postactivation treatment with latrunculin A (LatA), an actin polymerization inhibitor, on in vitro and in vivo development of somatic cell nuclear transfer (SCNT) embryos derived from kidney fibroblasts of an aged Clawn miniature boar (12 years old). After electric activation, SCNT embryos were treated with 0, 0.5 or 1 μM LatA and cultured in vitro. The rate of blastocyst formation was significantly higher (P<0.05) in SCNT embryos treated with 0.5 μM LatA (38%) than those in control (14%). When cloned embryos treated with 0.5 μM LatA were transferred into the oviducts of two recipient miniature gilts to assess their development in vivo, both recipients became pregnant; one maintained pregnancy to term, and a live piglet (weighing 220 g) was delivered by Caesarean section. The results of this study indicated that the postactivation treatment with LatA was effective in improving in vitro developmental capacity of SCNT miniature pig embryos derived from kidney fibroblasts of an aged animal and that miniature pig cloned embryos treated with LatA had the ability to develop to term. Key words: Aged donor, Clawn miniature pig, Cytoskeletal inhibitors, Latrunculin A, Nuclear transfer (J. Reprod. Dev. 58: [398][399][400][401][402][403] 2012) C lawn miniature pigs (inbred strain) have a potential value for biomedical research and xenotransplantation due to their clear genetic background [1][2][3] and few individual differences; thus, they have stimulated investigation of novel strategies directed at generating a genetically modified miniature pig by somatic cell nuclear transfer (SCNT). We have shown how to produce live offspring derived from Clawn miniature pig SCNT embryos; however, the efficiency still remains low [4]. To date, pigs have been cloned with SCNT using donor cells derived from fetal pigs to pigs several years of age. The efficiency of SCNT is influenced by many factors, including quality of recipient oocytes, type of donor cells, reprogramming of donor nuclei and condition of recipient gilts [4][5][6][7][8][9][10][11][12][13]. In particular, the diploid complement retention is one of the most important factors in improving the developmental ability of SCNT embryos [14,15]. SCNT embryos derived from diploid cells are generally treated with cytoskeletal inhibitors, such as cytochalasin B (CB), after activation to prevent extrusion of a pseudo polar body (pPB) and the possible loss of chromosomes maintain normal ploidy [7,16,17]. Several reports have shown that CB treatment of SCNT embryos resulted in increased blastocyst formation in vitro [18][19][20][21]. However, it has been reported that postactivation treatment with CB had no effect on either blastocyst rate or cell number of SCNT embryos [9,22]. CB possess cytotoxicity [23], and its cytotoxicity might have a detrimental effect on the subsequent development of SCNT embryos.Latrunculin A (LatA), a toxin purified from the Red Sea sponge Latrunculia magnifica, is a member of the cytoskel...

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Section: Discussionmentioning

confidence: 86%

Section: In Vitro Maturation Of Oocytesmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of Postactivation Treatment with Latrunculin A on In Vitro and In Vivo Development of Cloned Embryos Derived from Kidney Fibroblasts of an Aged Clawn Miniature Boar

Himaki

Mizobe

Tsuda

et al. 2012

Journal of Reproduction and Development

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show abstract

“…Actually, they reversibly disrupt microfilaments of cultured cells at submicromolar concentrations (Spector et al 1983). Latrunculin A binds to G-actin in a molar ratio of 1:1 with a K d value of 0.24 μM and sequesters G-actin from actin polymerization (Coué et al 1987). Since latrunculin A has no severing effect (Saito et al unpublished observation), its action seems similar to that of monomer-binding proteins such as thymosin β 4 or DNase I.…”

Section: Latrunculinsmentioning

confidence: 99%

Developmental Biology of Neoplastic Growth

Macieira‐Coelho¹

2005

Progress in Molecular and Subcellular Biology

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Effect of Low-Dose Latrunculin B on Anterior Segment Physiologic Features in the Monkey Eye

Okka¹,

Tian²,

Kaufman³

2004

Arch Ophthalmol

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To determine if low doses of topical latrunculin B (LAT-B) will increase outflow facility and decrease intraocular pressure without damaging the cornea and if they will inhibit miotic and accommodative responses to pilocarpine in monkeys. Methods: We measured intraocular pressure (Goldmann tonometry) before and after 1 and 9 doses of 0.005% and 0.01% topical LAT-B and vehicle given twice daily on successive weeks; outflow facility (perfusion) following 15 doses; central corneal thickness (ultrasonic pachymetry) before and after 1 and 9 doses of 0.01% LAT-B and vehicle; pupillary diameter (calipers); and accommodation (refractometry) before and after 1 dose of 0.005% and 0.02% LAT-B. Results: Latrunculin-B dose-dependently decreased intraocular pressure, multiple doses more than a single dose. Maximal mean Ϯ SEM hypotension after 1 dose was 2.5 Ϯ 0.3 mm Hg (0.005% LAT-B; n = 8; PϽ.001) or 2.7Ϯ 0.6 mm Hg (0.01% LAT-B; n = 8; PϽ.005); maximal meanϮSEM hypotension after 9 doses was 3.2Ϯ0.5 mm Hg (0.005% LAT-B; n = 8; PϽ.001) or 4.4 Ϯ 0.6 mm Hg (0.01% LAT-B; n = 8; PϽ.001). Outflow facility was increased by meanϮSEM 75%Ϯ13% (n=7; PϽ.005). Central corneal thickness was not changed after 1 or 9 doses of 0.01% LAT-B. Miotic and accommodative responses to intramuscular pilocarpine were dosedependently inhibited. With 0.02% LAT-B, inhibition of miosis was substantial, whereas the inhibition of accommodation was only about 25%. With 0.005% LAT-B, the effects were trivial. Conclusions: In ocular normotensive monkeys, 0.005% and 0.01% LAT-B administered topically increases outflow facility and/or decreases intraocular pressure without corneal effects. Multiple doses reduce intraocular pressure more than a single dose. Latrunculin-B dose-dependently relaxes the iris sphincter and ciliary muscle, with some separation of miotic and accommodative effects. Clinical Relevance: Multiple treatments with low topical doses of LAT-B may substantially reduce outflow resistance in eyes with glaucoma without adversely affecting the cornea.

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Inhibition of actin polymerization by latrunculin A

Cited by 772 publications

References 7 publications

Effect of Postactivation Treatment with Latrunculin A on <i>In Vitro</i> and <i>In Vivo</i> Development of Cloned Embryos Derived from Kidney Fibroblasts of an Aged Clawn Miniature Boar

Effect of Postactivation Treatment with Latrunculin A on <i>In Vitro</i> and <i>In Vivo</i> Development of Cloned Embryos Derived from Kidney Fibroblasts of an Aged Clawn Miniature Boar

Developmental Biology of Neoplastic Growth

Effect of Low-Dose Latrunculin B on Anterior Segment Physiologic Features in the Monkey Eye

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