Inhibition mechanisms of Fe2+/Fe3+ and Mn2+ on fungal laccase-enabled bisphenol a polyreaction

Li, Shunyao; Liu, Qingzhu; Liu, Jie; Sun, Kai; Yang, Wei; Si, Youbin; Liu, Yucheng; Gao, Yanzheng

doi:10.1016/j.chemosphere.2022.135685

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…The metal ions (Cu 2+ , Ca 2+ , Zn 2+ , Mg 2+ , K + , Fe 2+ , Na + , Mn 2+ ) utilized in this study were present at concentrations of 2.5 and 5.0 mM, respectively. A 60-microliter volume of a metal ion solution was combined with an equivalent volume of an enzyme solution to assess its impact on the laccase enzyme activity [22,23]. The relative enzyme activity is considered to be 100% upon mixing the enzyme solution with deionized water in a 1:1 ratio.…”

Section: Comprehensive Analysis Of Enzymatic Properties Of Laccasementioning

confidence: 99%

Comprehensive Analysis of Catalytic Characteristics and Molecular Mechanisms in Mutant Trametes versicolor Strains with Enhanced Laccase Activities

Zhang,

Yan,

et al. 2023

Fermentation

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The commercial potential of Trametes versicolor laccases in the degradation of various persistent contaminants is significant. Despite numerous attempts through rational metabolic engineering to enhance the properties of laccases, the outcomes have proven unsatisfactory for practical implementation. The present study successfully generated two novel mutants, namely, TA-04 and TA-15, derived from Trametes versicolor ATCC20869, utilizing atmospheric and room temperature plasma (ARTP). The laccase activities of TA-04 and TA-15 showed a significant increase to 136.507 ± 4.827 U/mg DCW and 153.804 ± 6.884 U/mg DCW, respectively, which were 1.201 and 1.354 times than that of the original strain. The laccase activities of the mutant strains TA-04 and TA-15 surpassed that of the original strain by 10.372% and 18.914%, respectively, at a higher pH level of five. Sequencing analysis of TA-04 and TA-15 revealed that several alternative amino acids within their active regions may enhance their catalytic characteristics under a higher temperature and pH condition. This study employed ARTP mutagenesis to propose two highly efficient microbial mutants derived from Trametes versicolor ATCC20869, exhibiting enhanced laccase activities. These mutants hold promising potential for the degradation of diverse environmental pollutants.

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Section: Comprehensive Analysis Of Enzymatic Properties Of Laccasementioning

confidence: 99%

Comprehensive Analysis of Catalytic Characteristics and Molecular Mechanisms in Mutant Trametes versicolor Strains with Enhanced Laccase Activities

Zhang,

Yan,

et al. 2023

Fermentation

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“…Similar dyes such as Brilliant Blue X-BR and Remazol Brilliant Blue R have been used for free and immobilized TvLac on chitosan beads [56]. Dithiothreitol, Fe(II) ions, and L-cysteine are well-known potent inhibitors of laccase that can potentially inhibit laccase bioremediation during waste treatment [36,57]. Therefore, decolorization of these dyes was assessed in the presence of these inhibitors.…”

Section: Decolorization Of Synthetic Dyes In the Presence Of Inhibito...mentioning

confidence: 99%

Trametes versicolor Laccase-Based Magnetic Inorganic-Protein Hybrid Nanobiocatalyst for Efficient Decolorization of Dyes in the Presence of Inhibitors

Patel,

Gupta,

Karuppanan

et al. 2024

Materials

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In the present investigation, an ecofriendly magnetic inorganic-protein hybrid system-based enzyme immobilization was developed using partially purified laccase from Trametes versicolor (TvLac), Fe3O4 nanoparticles, and manganese (Mn), and was successfully applied for synthetic dye decolorization in the presence of enzyme inhibitors. After the partial purification of crude TvLac, the specific enzyme activity reached 212 U∙mg total protein−1. The synthesized Fe3O4/Mn3(PO4)2-laccase (Fe3O4/Mn-TvLac) and Mn3(PO4)2-laccase (Mn-TvLac) nanoflowers (NFs) exhibited encapsulation yields of 85.5% and 90.3%, respectively, with relative activities of 245% and 260%, respectively, compared with those of free TvLac. One-pot synthesized Fe3O4/Mn-TvLac exhibited significant improvements in catalytic properties and stability compared to those of the free enzyme. Fe3O4/Mn-TvLac retained a significantly higher residual activity of 96.8% over that of Mn-TvLac (47.1%) after 10 reuse cycles. The NFs showed potential for the efficient decolorization of synthetic dyes in the presence of enzyme inhibitors. For up to five reuse cycles, Fe3O4/Mn-TvLac retained a decolorization potential of 81.1% and 86.3% for Coomassie Brilliant Blue R-250 and xylene cyanol, respectively. The synthesized Fe3O4/Mn-TvLac showed a lower acute toxicity towards Vibrio fischeri than pure Fe3O4 nanoparticles did. This is the first report of the one-pot synthesis of biofriendly magnetic protein-inorganic hybrids using partially purified TvLac and Mn.

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“…The addition of metal cations could effectively inhibit the mineralization and decomposition of the Hu component, among which Fe 3+ ions had the most significant effect, followed by Mn 2+ ions. Fe 3+ could restrain laccase activity because the formed Fe 3+ complex bound with the laccase site, interrupted the binding site of the enzyme, lowered the catalytic activity of fungal laccase, and thus impeded the single-electron transfer system, inhibiting the degradation of Hu by microorganisms [46]. Mn 2+ behaved as a cation bridge linking both the negatively charged minerals and humic acid, thereby increasing the stability of the mineral nanoparticles as a result of the steric repulsion of the adsorbed humic acid.…”

Section: Humentioning

confidence: 99%

Effect of Metal Cations with Different Valences on the Humus Composition of Dark-Brown Soil Mixed with Tilia Wood Shavings

Wang,

Liu,

Bai

et al. 2023

Agronomy

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To clarify the contribution of the bridging effect from three metal cations (K+, Mn2+, and Fe3+) on the humification of lignin-rich Tilia wood shavings and further enrich the theory of lignin humification, an indoor incubation method with constant temperature and humidity was adopted. K+, Mn2+, and Fe3+ served as additives, with CK as the control for studying the differential influence of metal cations with different valences on the humus composition of dark-brown soil mixed with Tilia wood shavings. The change in the C contents of water-soluble substance (CWSS), humic-extracted acid (CHE) and humin (CHu), ∆logK value of HE, atomic ratio and FTIR spectra of humic acid (HA), and the ratio of C content of humic acid to fulvic acid (CHA/CFA) of dark-brown soil mixed with Tilia wood shavings were analyzed after 0, 30, 80, and 150 days of incubation, and the following conclusions were reached: (1) The addition of metal cations, regardless of their valence, could effectively improve the microbial utilization and consumption of WSS, and the effect was as follows: Fe3+ > Mn2+ > K+. The addition of three metal cations could effectively inhibit mineralization and reduce the loss of TOC, and the effect could be seen as follows: Fe3+ > Mn2+ > K+. (2) Although the CHE content first decreased and then increased with incubation, the addition of Fe3+ and Mn2+ ions increased the CHE content, showing that Fe3+ > Mn2+, and K+ ions had no significant effect. Throughout the incubation, the structure of HE molecules changed first via a complex process and then through a simple process. Comparing the change before and after the incubation, the overall structure of HE molecules tended to be simpler with the CK control, and HE became more complicated with the addition of Fe3+ and Mn2+; however, the addition of K+ had little effect on the structure of HE molecules. (3) At the end of the incubation, the addition of Fe3+, Mn2+, and K+ ions strengthened the molecular condensation of HA and its aromatization degree, while the CK control without any added metal cations caused HA molecules to decompose and obtain a greater aliphatic degree. In addition, the number of O-containing functional groups and N-containing compounds in HA molecules increased to varying degrees regardless of which metal cation was added. The decomposition of Tilia wood chips led to a partial entry of the decomposition products into the HA component, which was then reconsumed by continuous mineralization. After incubation, the polysaccharides in HA molecules were consumed only with the addition of Mn2+ ions. Fe3+ and Mn2+ ions had greater advantages in increasing the CHA/CFA ratio and improving the humus quality than K+ ions. (4) The addition of metal cations could effectively inhibit the mineralization and decomposition of the Hu component, among which Fe3+ ions had the most significant effect, followed by Mn2+ ions. Compared to monovalent cations (K+), polyvalent cations (Fe3+ and Mn2+) had the advantage of a bridging effect, and their addition promoted the microbial utilization of WSS, effectively reduced the loss of TOC, increased the CHE content, complicated its molecular structure, improved the humus quality, and inhibited the decomposition of Hu. Regardless of which metal cation was added, the degree of molecular polycondensation and aromatization of HA was enhanced, and the number of O-functional groups and N-containing compounds in HA molecules increased.

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Inhibition mechanisms of Fe2+/Fe3+ and Mn2+ on fungal laccase-enabled bisphenol a polyreaction

Cited by 5 publications

References 48 publications

Comprehensive Analysis of Catalytic Characteristics and Molecular Mechanisms in Mutant Trametes versicolor Strains with Enhanced Laccase Activities

Comprehensive Analysis of Catalytic Characteristics and Molecular Mechanisms in Mutant Trametes versicolor Strains with Enhanced Laccase Activities

Trametes versicolor Laccase-Based Magnetic Inorganic-Protein Hybrid Nanobiocatalyst for Efficient Decolorization of Dyes in the Presence of Inhibitors

Effect of Metal Cations with Different Valences on the Humus Composition of Dark-Brown Soil Mixed with Tilia Wood Shavings

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