Inhibition mechanism of kojic acid on polyphenol oxidase

Abstract

The inhibition mode of kojic acid on mushroom, potato, apple, white shrimp, and spiny lobster polyphenol oxidase (PPO) was investigated. Using a polarographic method, kojic acid was shown to inhibit melanosis by interfering with the uptake of O2 required for enzymatic browning. Spectrophotometric and chromatographic methods demonstrated that kojic acid was capable of reducing o-quinones to diphenols to prevent the final pigment (melanin) forming. The preincubation temperature did not significantly affect (P > 0.05) PPO inhibition by kojic acid.

A Novel Inhibitor Against Mushroom Tyrosinase with a Double Action Mode and Its Application in Controlling the Browning of Potato

“…The ability of sample to reduce cupric copper to cuprous copper was determined according to the reported methods with minor modification (Andrawis and Kahn 1990;Chen et al 1991). Different concentrations of the sample solution (1 mL in DMSO) were first mixed with CuSO 4 solution (0.5 mL, 0.4 mM) in a centrifuge tube, and the mixture was incubated in a 25°C water bath for 10 min, then bathocuproine (0.5 mL, 4 mM) was added to this blend.…”

Influence of pH on Kojic Acid Fermentation by Aspergillus flavus

“…In food industry, kojic acid is used as a precursor for flavor enhancers (Le Blanch and Akers, 1989) and an anti-melanosis (blackening) of agriculture products during post-harvest by inhibiting polyphenol oxidase (Chen et al, 1991). Kojic acid is also used as an ingredient for whitening agent and a protective against UV light in cosmetics (Ohyama and Mishima, 1990).…”

2D-QSAR Analyses on The Tyrosinase Inhibitory Activity of 2-[(2,6-Dioxocyclohexyl)methyl]-cyclohexane-1,3-dione Analogues

“…그러므로 tyrosinase에 의한 산화를 억제하는 것은 피부미백에 있어서 매우 중요한 방법 중의 하나이다. Tyrosinase의 활성 저해제 로 가장 많이 알려져 있는 성분으로는 arbutin과 kojic acid 등이 있다 [4,5]. Arbutin은 hydroquinone에 당 분자 가 결합한 hydroquinone-O-β-D-glucopyranoside, (IUPAC; 2-(hydroxymethyl)-6-(4-hydroxyphenoxy)oxane-3,4,5-triol)로 tyrosine과 유사한 구조를 가지고 있어 tyrosinase 에 대한 경쟁적 저해제로 작용을 한다 [6].…”