Inhibition effects of Moutan Cortex Radicis on secretion of eotaxin in A549 human epithelial cells and eosinophil migration

Kim, Jin Ju; Lee, Hee Kyung; Lee, Young Seop; Oh, Bang Gul; Cho, Chong Woon; Kim, Yang Seok; Shin, Mikyung; Hong, Moo Chang; Jung, Sung Ki; Bae, Hyunsu

doi:10.1016/j.jep.2007.07.038

Cited by 34 publications

(17 citation statements)

References 27 publications

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“…(Li et al, 2007), the suppression effect of Paeonia suffruticosa Andr. on activated of NF-B (Kim et al, 2007), the inhibition effect of Celastrus orbiculatus Thunb. on synovial hyperplasia and cartilage erosion and degradation in severe combined immunodeficiency mice engrafted with human cartilage and rheumatoid synovium tissue (SCID-HuRAg) (Xiao et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effect of Sanshuibaihu decoction may be associated with nuclear factor-κB and p38 MAPKα in collagen-induced arthritis in rat

Yang¹,

Xiao²,

Wu³

et al. 2010

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 99%

Anti-inflammatory effect of Sanshuibaihu decoction may be associated with nuclear factor-κB and p38 MAPKα in collagen-induced arthritis in rat

Yang¹,

Xiao²,

Wu³

et al. 2010

Journal of Ethnopharmacology

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“…Recently, it has been reported that airway epithelial cells not only act as physical barrier but also produce several cytokines (Zhang et al,2011) and chemokines, including IL-8, RANTES, and eotaxin (Kim et al, 2007;Yoshikawa et al, 2010), which contribute directly or indirectly to the local immune response and accumulation of inflammatory cells. In this study, we have demonstrated that A549 cell line, which is often used as a representative model of airway epithelial cells, can release IL-8, RANTES and eotaxin.…”

Section: Discussionmentioning

confidence: 99%

Distribution Change of Mast Cells in Human Nasal Polyps

Zhang

Shao

et al. 2012

The Anatomical Record

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Recent studies have shown that mast cells are involved in pathophysiologic processes of chronic inflammation. However, little is known about the distribution of mast cells in nasal polyps, which is a chronic inflammatory disease of the upper airways. Biopsy specimens from patients with nasal polyps (n ¼ 20) and control patients without nasal polyps (n ¼ 8) were included in this study. The distribution of mast cells in nasal polyps was determined by immunohistochemistry. Meanwhile, we detected the expression of chemokines (CCL5, CCL11, CX3CL1, IL-8, IL-6) in the epithelial cells of normal nasal mucosa and nasal polyps. In addition, the expression of these chemokines was investigated by western bolting in airway epithelial cells line (A549 cells) under inflammatory condition. Mast cells migrated toward intraepithelium in nasal polyps and the expression of chemokines (CCL5, CCL11, CX3CL1, IL-8) was up-regulated in the epithelial cells of nasal polyps compared with normal nasal mucosa. The expression of chemokines was also up-regulated in A549 cells after Lipopolysaccharide (LPS)-treatment for 3 hr and 6 hr. Our findings showed that mast cells migrate toward intraepithelium in nasal polyps and the overexpression of chemokines (CCL5, CCL11, CX3CL1, IL-8) suggested that they might be responsible for mast cells migration. It implies that mast cell play potential roles in the development of nasal polyps.

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“…Eotaxin is also detected in airway epithelial cells, and its presence is increased in asthma [6] . Moreover, the level of eotaxin is associated with the degree of subepithelial fibrosis and eosinophilic airway inflammation [7] . In this study, we found that eotaxin secretion was significantly inhibited by treatment with EC ( fig.…”

Section: Discussionmentioning

confidence: 99%

Global Gene Analysis of <i>Erigeron canadensis-</i>Treated TNF-α-, IL-4- and IL-1β-Stimulated A549 Human Epithelial Cells

Sohn

Ko²,

Oh³

et al. 2009

Ann Nutr Metab

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Background/Aims: This study was conducted to evaluate the anti-inflammatory mechanisms of Erigeron canadensis (EC) on the tumor necrosis factor-α (TNF-α)-, interleukin (IL)-4- and IL-1β-induced stimulation of A549 cells. Methods: In the present study, the anti-inflammatory effects of EC on TNF-α-, IL-4- and IL-1β-induced A549 cells were determined by analyzing eotaxin secretion using ELISA. In addition, the effects of ECon gene expression profiles in stimulated A549 cells were evaluated by microarray analysis. Results: Oligonucleotide microarray analysis revealed that inflammatory-related genes such as NOS1, NOS2A, IL-1β, IL-8 and CSF2 and cell adhesion-related genes such as SELE, MMP3, VCAM1, ICAM1, ITGA7 and ITGB2 were downregulated in EC-treated A549 cells that had been pretreated with TNF-α, IL-4 and IL-1β. In addition, significant decreases in Eotaxin, ICAM, VCAM and IL-8 gene expression were observed in EC-treated A549 cells. Conclusions: EC has an anti-inflammatory effect in stimulated A549 cells. Microarray-based genomic survey is a high-throughput approach that is suitable for the evaluation of gene expression in cell lines that have been treated with EC.

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Inhibition effects of Moutan Cortex Radicis on secretion of eotaxin in A549 human epithelial cells and eosinophil migration

Cited by 34 publications

References 27 publications

Anti-inflammatory effect of Sanshuibaihu decoction may be associated with nuclear factor-κB and p38 MAPKα in collagen-induced arthritis in rat

Anti-inflammatory effect of Sanshuibaihu decoction may be associated with nuclear factor-κB and p38 MAPKα in collagen-induced arthritis in rat

Distribution Change of Mast Cells in Human Nasal Polyps

Global Gene Analysis of <i>Erigeron canadensis-</i>Treated TNF-α-, IL-4- and IL-1β-Stimulated A549 Human Epithelial Cells

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