Inhibition by α‐amanitin of the oestradiol‐induced increase in α‐amanitin insensitive RNA polymerase in immature rat uterus

Raynaud-Jammet, C.; Catelli, Maria-Grazia; Baulieu, Étienne Émile

doi:10.1016/0014-5793(72)80228-x

Cited by 30 publications

(4 citation statements)

References 17 publications

(9 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This early stimulation of putative mRNA synthesis (Knowler and Smellie, 1971;Glasser et al, 1972;Borthwick and Smellie, 1975) may be involved in the subsequent synthesis of uterine proteins involved in the later increase of both polymerases and, in turn, other proliferative changes which characterize the uterotrophic action of estrogen. A similar suggestion was made by Raynaud-Jammet et al (1972) who found an RNA product whose synthesis, which was sensitive to aamanitin, appeared necessary for the increase in RNA polymerase I activity which followed estradiol administration. Our present results would indicate, however, that the action of estrogen on RNA synthesis may be more complex and difficult to interpret than simply relating an early effect in RNA polymerase II activity to the synthesis of a product that modulates the later increases in the activities of the polymerases.…”

Section: Discussionsupporting

confidence: 73%

RNA polymerase activity and uterine growth: differential stimulation by estradiol, estriol, and nafoxidine

Hardin¹,

Clark²,

Glasser³

et al. 1976

Biochemistry

120

View full text Add to dashboard Cite

We have shown previously that estradiol, estriol, and Nafoxidine (Upjohn 11, 100A) have differential effects on uterine growth and that these effects are associated with the retention of the estrogen receptor by the nucleus. In order to examine these relationships further, we have studied the effect of these hormones on endogenous nuclear RNA polymerase I and II in the immature rat uterus. All three compounds caused a rapid elevation in polymerase II activity that reached a peak by the first hour and declined to almost control levels by 2 h after the injection. This transient peak in polymerase II activity was followed by a second elevation by the fourth hour in estradiol- and Nafoxidine-treated animals which was not observed in estriol-treated rats. The activity of polymerase I increased monotonically to very high levels by 4 h and was maintained 12 h or longer in estradiol- and Nafoxidine-treated animals. A similar elevation was observed in estriol-treated rats but the activity declined very rapidly to control levels by 12 h. The second elevation in polymerase II activity and the sustained stimulation of polymerase I activity were correlated with the stimulation of true uterine growth. These data confirm our previous suggestion that long-term nuclear retention of the receptor is a requirement for true uterine growth and suggest that an obligatory response in the production of true growth is the stimulation of a second rise in polymerase II activity and an elevated and sustained activity of polymerase I.

show abstract

Section: Discussionsupporting

confidence: 73%

RNA polymerase activity and uterine growth: differential stimulation by estradiol, estriol, and nafoxidine

Hardin¹,

Clark²,

Glasser³

et al. 1976

Biochemistry

120

View full text Add to dashboard Cite

show abstract

“…Evidence is now accumulating that growth-stimulating hormones do produce increased activity of the nucleolar polymerase I in the responsive tissue (Smuckler & Tata, 1971;Yu & Feigelson, 1971;Sadjel & Jacob, 1971;Mainwaring et al, 1971). These observations are consistent with the demonstration that the nucleolar polymerase is a metabolically labile species that is turned over with considerable rapidity (Yu & Feigelson, 1972;Raynaud-Jammet et al, 1972), making it ideally suited for such a regulatory role. Generalizing from our own findings and those of others (Smuckler & Tata, 1971;Chesterton et al, 1972), the amount of the nucleoplasmic enzyme may be more constant in a given cell with control of nucleoplasmic RNA synthesis being exerted at other levels of organization.…”

Section: Discussionsupporting

confidence: 88%

Deoxyribonucleic acid-dependent ribonucleic acid polymerases from murine spleen cells. Increased amounts of the nucleolar species in leukaemic tissue

Babcock

Rich

1973

Biochemical Journal

View full text Add to dashboard Cite

1. In the spleens of infected mice, the Friend leukaemia virus induces a sharp increase in the ability of subsequently isolated nuclei to incorporate exogenous UTP into an acid-insoluble product. Inhibitor studies indicate that the incremental RNA synthesis proceeds from a DNA template and that both nucleolar and nucleoplasmic activities are involved. 2. The partially purified DNA-dependent RNA polymerases from control and virus-infected tissue are indistinguishable with respect to chromatographic mobility, dependence on bivalent cations, ionic strength, pH and their susceptibility to alpha-amanitin. The RNA polymerases of the murine spleen resemble the enzymes of other mammalian tissue in these properties. 3. A comparison of the amount of polymerase solubilized from normal and infected tissue correlates with the activity observed in assays of the respective nuclei. These experiments indicated that the increase in nucleolar RNA synthesis after infection is mediated by increased extractable polymerase I activity whereas the change in nucleoplasmic RNA synthesis results from an alteration of chromatin or a chromatin-associated factor.

show abstract

“…More recent studies on the RNA polymerases of eukaryotic cells have revealed the presence of at least two classes of enzyme, RNA polymerase A and RNA polymerase B (Chambon et al, 1970;Roeder & Rutter, 1970;Chesterton & Butterworth, 1971;Chesterton et al, 1972). Previous measurements of the RNA polymerase activities of nuclei from the uteri of immature or ovariectomized rats have shown that oestradiol causes an increase in the endogenous RNA polymerase A activity 1-2h after hormone treatment although no changes were detected in the activity of RNA polymerase B during the first 12h (Barry & Gorski, 1971;Raynaud-Jammet et al, 1972).…”

Section: Vol 147mentioning

confidence: 99%

The effects of oestradiol-17β on the ribonucleic acid polymerases of immature rabbit uterus

Borthwick

Smellie

1975

Biochemical Journal

View full text Add to dashboard Cite

Measurements of the endogenous RNA polymerase activities of nuclei isolated from immature rabbit uteri have shown that prior treatment of the animals with oestradiol-17beta has a profound effect on the apparent activities of both RNA polymerases A and B. Within 1 h of hormone treatment, the activity of RNA polymerase A is increased and continues to rise until about 4h when it reaches a plateau and remains steady until at least 8h. The activity of RNA polymerase B increases sharply after oestradiol treatment reaching an early maximum at 30-45 min. Thereafter this activity declines until by 1-2h it approaches control values but a second increase in activity then occurs with a maximum at 3-4h. Treatment of the rabbits with alpha-amanitin before the administration of oestradiol inhibits the hormone-induced stimulation of RNA polymerase A activity in isolated nuclei but when the administration of alpha-amanitin is delayed until after the early rise of RNA polymerase B activity, the oestradiol-induced stimulation of RNA polymerase A is retained. Similar results have been obtained in experiments with cycloheximide suggesting that the stimulation of RNA polymerase A activity by oestradiol is dependent on the hormone-induced stimulation of RNA polymerase B and the subsequent synthesis of protein using the RNA product of the early increase in RNA polymerase B activity. Measurement of the activities of RNA polymerases A and B after isolation of the enzymes from immature rabbit uterine nuclei before and after oestradiol treatment failed to show any differences. Therefore it would appear that the changes in the observed activities of RNA polymerases A and B in isolated nuclei are consequences of changes in the structure and function of chromatin rather than the results of modifications in the RNA polymerases themselves.

show abstract

Inhibition by α‐amanitin of the oestradiol‐induced increase in α‐amanitin insensitive RNA polymerase in immature rat uterus

Cited by 30 publications

References 17 publications

RNA polymerase activity and uterine growth: differential stimulation by estradiol, estriol, and nafoxidine

RNA polymerase activity and uterine growth: differential stimulation by estradiol, estriol, and nafoxidine

Deoxyribonucleic acid-dependent ribonucleic acid polymerases from murine spleen cells. Increased amounts of the nucleolar species in leukaemic tissue

The effects of oestradiol-17β on the ribonucleic acid polymerases of immature rabbit uterus

Contact Info

Product

Resources

About