Inhibition by TRPA1 agonists of compound action potentials in the frog sciatic nerve

Matsushita, Akitomo; Ohtsubo, Satoshi; Fujita, Teizo; Kumamoto, Eiichi

doi:10.1016/j.bbrc.2013.02.127

Cited by 18 publications

(31 citation statements)

References 19 publications

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“…; Matsushita et al. ). In brief, either sex of frogs ( Rana nigromaculata ) was decapitated and then pithed; thereafter the sciatic nerve was dissected from the lumbar plexus to the knee in Ringer solution.…”

Section: Methodsmentioning

confidence: 97%

“…; Matsushita et al. ). The peak amplitude of the CAP depended on the strength of the stimulus given to the sciatic nerve as such that the CAP peak amplitude enhanced with an increase in stimulus strength and attained a maximal value.…”

Section: Methodsmentioning

confidence: 97%

“…; Matsushita et al. ), we analyzed the peak amplitude of the maximal CAP. A conduction velocity value was determined by using the fifth electrode as an additional stimulation site.…”

Section: Methodsmentioning

confidence: 99%

“…), that activates TRP melastatin‐8 (TRPM8) channels, and also by a wasabi component allyl isothiocyanate and a cinnamon component cinnamaldehyde (Matsushita et al. ), both of which are TRP ankyrin‐1 (TRPA1) agonists. Although some of plant‐derived aroma oil compounds, such as citral, camphor, and (+)‐borneol, activate TRP channels (Moqrich et al.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of the compound action potentials of frog sciatic nerves by aroma oil compounds having various chemical structures

Ohtsubo

Fujita

Matsushita

et al. 2015

Pharmacology Res & Perspec

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Plant-derived chemicals including aroma oil compounds have an ability to inhibit nerve conduction and modulate transient receptor potential (TRP) channels. Although applying aroma oils to the skin produces a local anesthetic effect, this has not been yet examined throughly. The aim of the present study was to know how nerve conduction inhibitions by aroma oil compounds are related to their chemical structures and whether these activities are mediated by TRP activation. Compound action potentials (CAPs) were recorded from the frog sciatic nerve by using the air-gap method. Citral (aldehyde), which activates various types of TRP channels, attenuated the peak amplitude of CAP with the half-maximal inhibitory concentration (IC50) value of 0.46 mmol/L. Another aldehyde (citronellal), alcohol (citronellol, geraniol, (±)-linalool, (−)-linalool, (+)-borneol, (−)-borneol, α-terpineol), ester (geranyl acetate, linalyl acetate, bornyl acetate), and oxide (rose oxide) compounds also reduced CAP peak amplitudes (IC50: 0.50, 0.35, 0.53, 1.7, 2.0, 1.5, 2.3, 2.7, 0.51, 0.71, 0.44, and 2.6 mmol/L, respectively). On the other hand, the amplitudes were reduced by a small extent by hydrocarbons (myrcene and p-cymene) and ketone (camphor) at high concentrations (2–5 mmol/L). The activities of citral and other TRP agonists ((+)-borneol and camphor) were resistant to TRP antagonist ruthenium red. An efficacy sequence for the CAP inhibitions was generally aldehydes ≥ esters ≥ alcohols > oxides >> hydrocarbons. The CAP inhibition by the aroma oil compound was not related to its octanol–water partition coefficient. It is suggested that aroma oil compounds inhibit nerve conduction in a manner specific to their chemical structures without TRP activation.

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Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

“…; Matsushita et al. ), we analyzed the peak amplitude of the maximal CAP. A conduction velocity value was determined by using the fifth electrode as an additional stimulation site.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Inhibition of the compound action potentials of frog sciatic nerves by aroma oil compounds having various chemical structures

Ohtsubo

Fujita

Matsushita

et al. 2015

Pharmacology Res & Perspec

Self Cite

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show abstract

“…Similar CAP inhibitions were also produced by TRPA1 agonists. AITC and CA concentration-dependently reduced the peak amplitude of the CAP with the IC 50 values of 1.5 and 1.2 mM, respectively, and these activities were resistant to ruthenium red or HC-030031, indicating no involvement of TRPA1 channels [143]. The efficacies of the TRP agonists in inhibiting CAPs were almost comparable to those of local anesthetics such as lidocaine and procaine.…”

Section: Actions Of Plant-derived Trp Agonists On Synaptic Transmimentioning

confidence: 99%

TRP Channels Involved in Spontaneous L-Glutamate Release Enhancement in the Adult Rat Spinal Substantia Gelatinosa

Kumamoto

Fujita

Jiang

2014

Cells

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The spinal substantia gelatinosa (SG) plays a pivotal role in modulating nociceptive transmission through dorsal root ganglion (DRG) neurons from the periphery. TRP channels such as TRPV1 and TRPA1 channels expressed in the SG are involved in the regulation of the nociceptive transmission. On the other hand, the TRP channels located in the peripheral terminals of the DRG neurons are activated by nociceptive stimuli given to the periphery and also by plant-derived chemicals, which generates a membrane depolarization. The chemicals also activate the TRP channels in the SG. In this review, we introduce how synaptic transmissions in the SG neurons are affected by various plant-derived chemicals and suggest that the peripheral and central TRP channels may differ in property from each other.

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Ingestion of transient receptor potential channel agonists attenuates exercise‐induced muscle cramps

et al. 2017

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Exercise associated muscle cramping (EAMC) is a poorly understood problem that is neuromuscular in origin. Ingestion of TRP channel agonists has been efficacious in attenuating electrically-induced muscle cramps. Purpose To examine the effect of TRP agonist ingestion on voluntarily-induced EAMC and motor function. Methods Study 1: 39 subjects completed 2 trials after ingesting TRP agonist-containing active treatment (A), or vehicle (V) control. Cramping in the triceps surae was induced via voluntary isometric contraction. Study 2: After ingesting A or V, 31 subjects performed kinematic and psychomotor tests of manual dexterity. Results A increased pre-cramp contraction duration (A:36.9±4.1, V:27.8±3.1 s), decreased cramp EMG area under the curve (A:37.3±7.7, V:77.2±17.7 %EMGmax•s), increased contraction force to produce the cramp (A:13.8±1.8, V:9.9±1.6 kg), and decreased post-cramp soreness (A:4.1±0.3, V:4.7±0.3 a.u.). Kinematic and psychomotor tests were not affected. Discussion TRP agonist ingestion attenuated EAMC characteristics without affecting motor function.

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Inhibition by TRPA1 agonists of compound action potentials in the frog sciatic nerve

Cited by 18 publications

References 19 publications

Inhibition of the compound action potentials of frog sciatic nerves by aroma oil compounds having various chemical structures

Inhibition of the compound action potentials of frog sciatic nerves by aroma oil compounds having various chemical structures

TRP Channels Involved in Spontaneous L-Glutamate Release Enhancement in the Adult Rat Spinal Substantia Gelatinosa

Ingestion of transient receptor potential channel agonists attenuates exercise‐induced muscle cramps

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