Inhibition by acetylene of ammonia oxidation in Nitrosomonas europaea

Hynes, Russell K.

doi:10.1016/0378-1097(78)90146-5

Cited by 61 publications

(79 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Denitrification rates measured using C 2 H 2 blockage were all much lower than all of the rates of Eyre et al (2008) and those measured during this study (Table 4). The lower rates measured using C 2 H 2 blockage most likely reflect inhibition of nitrification (Hynes and Knowles, 1978), which would result in rates being underestimated because of the strong nitrification-denitrification coupling (see later discussion). In addition, in the C 2 H 2 blockage slurries there is no advective pore water flow, which appears to enhance coupled nitrification-denitrification in permeable carbonate sediments Santos et al, 2012a).…”

Section: Comparison Of Denitrification Rates In Carbonate Sandsmentioning

confidence: 99%

“…2602 B. D. Eyre et al: Seasonal, daily and diel N 2 effluxes in permeable carbonate sediments to 19 µmol N 2 -N m −2 h −1 ) most likely reflects the C 2 H 2 blockage technique underestimating coupled nitrificationdenitrification due to inhibition of nitrification (Hynes and Knowles, 1978). More recently higher direct N 2 fluxes (3 to 94 µmol N 2 -N m −2 h −1 ) have been measured in the dark using the background removal technique (Nowicki, 1994) in fine to medium carbonate sands at inter-reef sites on the northern and central Great Barrier Reef (GBR) (Alongi et al, 2008) and in very coarse carbonate sands in the southern GBR (Alongi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Seasonal, daily and diel N<sub>2</sub> effluxes in permeable carbonate sediments

2013

View full text Add to dashboard Cite

Abstract. Benthic metabolism and inorganic nitrogen and N2 flux rates (denitrification) were measured in permeable carbonate sands from Heron Island (Great Barrier Reef). Some of the N2 flux rates were among the highest measured in sediments. All benthic fluxes showed a significant difference between seasons with higher rates in summer and late summer. There was no distinct response of the benthic system to mass coral spawning. Instead, changes in benthic fluxes over 12 days in summer appear to be driven by tidal changes in water depth and associated changes in phytosynthetically active radiation reaching the sediments. Dark N2 fluxes were strongly correlated to benthic oxygen consumption across all sites and seasons (r2 = 0.63; p < 0.005; slope = 0.035). However, there were seasonal differences with a steeper slope in summer than winter, reflecting either more efficient coupling between respiration and nitrification–denitrification at higher temperatures or different sources of organic matter. Adding data from published studies on carbonate sands revealed two slopes in the dark N2 flux versus benthic oxygen consumption relationship. The lower slope (0.035) was most likely due to high carbon : nitrogen (C : N) organic matter from coral reefs, and associated assimilation of nitrogen by heterotrophic bacteria including enhanced heterotrophic N-fixation, but competition by benthic microalgae or inefficient coupling between respiration and nitrification–denitrification cannot be excluded. The steeper slope (0.089) was most likely due to respiration being driven by low C : N phytodetritus. If the different slopes were driven by the sources of organic matter, then global estimates of continental shelf denitrification are probably about right. In contrast, global estimates of continental shelf denitrification may be over-estimated if the low slope was due to inefficient coupling between respiration and nitrification–denitrification and also due to reduced N2 effluxes in the light associated with competition by benthic microalgae for nitrogen and N-fixation.

show abstract

Section: Comparison Of Denitrification Rates In Carbonate Sandsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Seasonal, daily and diel N<sub>2</sub> effluxes in permeable carbonate sediments

2013

View full text Add to dashboard Cite

show abstract

“…The link between N 2 O emission and amount of N-fertilizer applied has given rise to the concept of the emission factor (EF), where EF is the amount of N 2 O-N emitted expressed as a fraction (or a percentage) of the N applied [28]. Therefore, N 2 O and CH 4 emission rates are studied in the incubated soil samples by applying various external N sources.…”

Section: Variation Of Nitrogen Sourcementioning

confidence: 99%

Role of Microorganisms in Emission of Nitrous Oxide and Methane in Pulse Cultivated Soil Under Laboratory Incubation Condition

et al. 2012

View full text Add to dashboard Cite

“…Acetylene (C 2 H 2 ) is a suicide substrate for AMO (Hynes and Knowles 1978), and thus inhibits ammonia oxidation by autotrophic nitrifiers (Jia and Conrad 2009) at low concentration (10-100 Pa) and inhibits N 2 O reductase during denitrification at high concentration (10 KPa) (Huang et al 2014b;Cui et al 2016). Inhibition of soil ammonia oxidation by C 2 H 2 is also used in routine methods to distinguish N 2 O production by nitrifiers and denitrifiers in soils (Bateman and Baggs 2005;Zhu et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

et al. 2016

View full text Add to dashboard Cite

Nitrification is believed to be one of the major sources of N 2 O production emitted from soil. Previous studies showed that both ammonia-oxidising bacteria (AOB) and archaea (AOA) can produce N 2 O via nitrification but their relative contributions are still poorly defined. Here, we used acetylene, an inhibitor of AOB and AOA ammonia monooxygenase (AMO), and 1-octyne, a selective inhibitor that specifically inhibits AOB AMO, to investigate how AOB versus AOA contribute to N 2 O emissions in two distinct arable soils. Soil amended with ammonium (NH 4 + ) increased N 2 O emissions to a greater extent than nitrate (NO 3 − ), and acetylene had a greater impact on N 2 O emissions in NH 4 + -treated soils than that in NO 3 − -amended soils, which indicated that nitrification was the dominant N 2 O emitting process in these two arable soils. In the alluvial and red soil, the percentage of evolved N 2 O after application of NH 4 + by AOB were 70.5~78.1 % and 18.7~19.7 % by AOA, respectively. Quantitative PCR revealed that NH 4 + addition stimulated AOB growth, and the growth could be significantly inhibited by acetylene or 1-octyne in the two soils. The stimulation of N 2 O emissions by NH 4 + and the relative suppression by inhibitors paralleled fluctuations in the AOB growth. In addition, cumulative N 2 O emissions were not correlated with AOA abundance in the two soils. Our results revealed that AOB could contribute more to soil N 2 O production than AOA in the NH 4 + -amended arable soils.

show abstract

Inhibition by acetylene of ammonia oxidation in Nitrosomonas europaea

Cited by 61 publications

References 3 publications

Seasonal, daily and diel N<sub>2</sub> effluxes in permeable carbonate sediments

Seasonal, daily and diel N<sub>2</sub> effluxes in permeable carbonate sediments

Role of Microorganisms in Emission of Nitrous Oxide and Methane in Pulse Cultivated Soil Under Laboratory Incubation Condition

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

Contact Info

Product

Resources

About

Inhibition by acetylene of ammonia oxidation in Nitrosomonas europaea

Cited by 61 publications

References 3 publications

Seasonal, daily and diel N&lt;sub&gt;2&lt;/sub&gt; effluxes in permeable carbonate sediments

Seasonal, daily and diel N&lt;sub&gt;2&lt;/sub&gt; effluxes in permeable carbonate sediments

Role of Microorganisms in Emission of Nitrous Oxide and Methane in Pulse Cultivated Soil Under Laboratory Incubation Condition

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

Contact Info

Product

Resources

About

Seasonal, daily and diel N<sub>2</sub> effluxes in permeable carbonate sediments

Seasonal, daily and diel N<sub>2</sub> effluxes in permeable carbonate sediments