Inhibition and neuromuscular paralysis in <i>Ascaris lumbricoides</i>

Goodwin, L. G.; Williams, E. M. Vaughan

doi:10.1113/jphysiol.1963.sp007227

Cited by 16 publications

(5 citation statements)

References 5 publications

(7 reference statements)

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Section: Drug Inhibition Of Ascaris Musclesupporting

confidence: 87%

“…In many Ascaris preparations, the stimulation of the nerve cord region with carefully placed electrodes gives rise to phasic hyperpolarizations which bring the membrane potential above 40 mV, thus suppressing the generation of rhythmic spike potentials (del Castillo et al, 1963b). These muscle inhibitory potentials, which will be described in detail elsewhere, seem to be due to the stimulation of nerve cord fibres, in agreement with the observations of Goodwin & Vaughan Williams (1963) Control record (uppermost), taken in 30% sea water; resting potential 27 mV; average spike amplitude just below this value; frequency about 2.9 spikes/sec. Middle record, taken about 1 min after addition of piperazine (5 x 10-4); the spike frequency had decreased to about 1.6 spikes/sec, while the average amplitude had decreased only slightly.…”

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confidence: 73%

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Mechanism of the Paralysing Action of Piperazine on Ascaris Muscle

Castillo¹,

Mello²,

Morales³

1964

British Journal of Pharmacology and Chemotherapy

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The effects of piperazine on Ascaris muscle cells have been investigated with electrophysiological techniques. These cells have an average resting potential of about 30 mV interrupted by rhythmic spikes of myogenic origin (1 to 7 spikes/sec). With piperazine (10−3, w/v), the average resting potential increases above 40 mV and the pacemaker activity is suppressed. These changes are similar to those temporarily produced in the same cells by the electrical stimulation of inhibitory nerve fibres. Electrophoretic application of piperazine to different areas of the muscle cells shows that this drug hyperpolarizes their membrane only when applied to the region where both excitatory and inhibitory neuromuscular synapses are located. The degree of muscle hyperpolarization induced by piperazine depends upon the extracellular chloride concentration, decreasing when a fraction of the chloride ions is replaced by the larger, supposedly nonpenetrating, sulphate anions. Piperazine may, therefore, be regarded as a pharmacological analogue of a natural inhibitory transmitter.

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Section: Drug Inhibition Of Ascaris Musclesupporting

confidence: 87%

supporting

confidence: 73%

Mechanism of the Paralysing Action of Piperazine on Ascaris Muscle

Castillo¹,

Mello²,

Morales³

1964

British Journal of Pharmacology and Chemotherapy

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“…that muscular contraction in nematodes is myogenic in origin but the original stimulus is probably nervous in origin. Opposed pairs of excitatory and inhibitory nerve fibres modulate membrane potentials and the rates of firing of spikes (Goodwin & Vaughan Williams, 1963). Acetylcholine is probably the transmitter of the excitatory fibres and y-amino butyric acid is thought to be the transmitter of the inhibitory fibres.…”

Section: N a Croll And Ismail Al-hadithimentioning

confidence: 99%

Sensory basis of activity in Ancylostoma tubaeforme infective larvae

Croll

Al-Hadithi

1972

Parasitology

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Upon stimulation, following a period of ambient conditions, infective A. tubaeforme larvae follow a committed characteristic pattern of activity, after being released by short-term mechanical or continuous photic stimulation. For the photic response this is stimulated by an increase in the intensity of stimulation, and for non-dark-adapted larvae a sudden fivefold increase in illumination is required, irrespective of previous stimulation.Acetylcholine chloride, adenosine triphosphate, γ-amino butyric acid, histamine diphosphate, 5-hydroxytryptamine, neostigmine bromide, physostigmine (eserine), potassium hydroxide, succinyl choline chloride and d-tubocurarine chloride have been added to larvae and their subsequent postures and activity recorded. It is concluded that the typical activity pattern of larvae may be altered by the use of drugs which interfere with neuromuscular phenomena, and therefore the activity responses of infective A. tubaeforme are based on sensory and neurosecretory rather than energetic considerations.We thank Mrs Helen Foreman for maintaining the larvae and the Medical Research Council of Great Britain for their generous support.

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“…Piperazine inhibits both the electrical and motor activities of Ascaris, and this inhilbition may be reversed by washing (del Castillo et al, 1963;Standen, 1955). The inhibition of motor activity resulting from stimulation of the " head" of the worm (Goodwin & Vaughan Williams, 1963) was shown not to be involved in the inhilbitory action of piperazine (del Castillo et al, 1963).…”

mentioning

confidence: 99%

The pharmacology of the cholinoceptor in muscle preparations of Ascaris lumbricoides var. suum

Natoff

1969

British J Pharmacology

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. The preparation of a muscle strip of Ascaris lumbricoides for the study of the effect of drugs in vitro is described. . Stimulant drugs which are classified as nicotine‐like in mammalian pharmacology increased the isometric tension of this preparation. These drugs were, in descending order of potency: dimethylphenylpiperazinium, nicotine, acetylcholine, carbachol, decamethonium and pyridine‐2‐aldoxime methiodide. . Muscarine‐like drugs (oxotremorine, methacholine, pilocarpine) had no activity. . Potassium and barium ions stimulated the tissue, while the anti‐cholin‐esterases, dichlorvos and eserine, increased the resting tension of the preparation and potentiated the responses to acetylcholine. . Adrenaline neither stimulated the tissue nor affected the responses to nicotine‐like drugs. . The relative potency of several blocking agents which antagonize the responses to nicotine‐like drugs was assayed. These blocking agents were, in descending order of potency: mecamylamine, (+)‐tubocurarine, hexamethonium, atropine and piperazine. Acetylcholine, dimethylphenylpiperazinium and pyridine‐2‐aldoxime methiodide apparently act on a common receptor, for each blocking agent had a similar molar inhibitory concentration against these stimulants. . It is concluded that the cholinoceptor in muscle preparations of Ascaris lumbricoides is pharmacologically similar to that of the mammalian autonomic ganglion.

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Inhibition and neuromuscular paralysis in Ascaris lumbricoides

Cited by 16 publications

References 5 publications

Mechanism of the Paralysing Action of Piperazine on Ascaris Muscle

Mechanism of the Paralysing Action of Piperazine on Ascaris Muscle

Sensory basis of activity in Ancylostoma tubaeforme infective larvae

The pharmacology of the cholinoceptor in muscle preparations of Ascaris lumbricoides var. suum

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