Inhibition and destruction of Pseudomonas aeruginosa biofilms by antibiotics and antimicrobial peptides

Döşler, Sibel; Karaaslan, Elif

doi:10.1016/j.peptides.2014.09.021

Cited by 181 publications

(169 citation statements)

References 24 publications

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“…Most of these compounds take part in the prevention of biofilm formation, but little information is available regarding compounds that are able to disrupt established wound biofilm. One of the potential agents responsible for biofilm destruction is bee (Apis melifera)-derived antibacterial peptide defensin-1 (Def-1), as it has been shown recently that antimicrobial cationic peptides destroy bacterial biofilm (Dosler & Mataraci, 2013;Dosler & Karaaslan, 2014). Def-1 is one of the main regular but quantitatively variable antibacterial components of honey (Kwakman et al, 2011;Majtan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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Many clinically relevant biofilms are polymicrobial. Examining the effect of antimicrobials in a multispecies biofilm consortium is of great clinical importance. The goal of this study was to investigate the effect of different honey types against bacterial wound pathogens grown in multispecies biofilm and to test the antibiofilm activity of honey defensin-1 (Def-1) in its recombinant form. A modified Lubbock chronic wound biofilm formed by four bacterial species (Staphylococcus aureus, Streptococcus agalactiae, Pseudomonas aeruginosa and Enterococcus faecalis) was used for evaluation of honey and recombinant bee-derived Def-1 antibiofilm efficacy. Recombinant Def-1 was prepared by heterologous expression in Escherichia coli. We showed that different types of honey (manuka and honeydew) were able to significantly reduce the cell viability of wound pathogens (Staphylococcus aureus, Streptococcus agalactiae and Pseudomonas aeruginosa) in mature polymicrobial biofilm. None of the tested honeys showed the ability to eradicate Enterococcus faecalis in biofilm. In addition, recombinant Def-1 successfully reduced the viability of Staphylococcus aureus and Pseudomonas aeruginosa cells within established polymicrobial biofilm after 24 and 48 h of treatment. Interestingly, recombinant Def-1 did not affect the viability of Streptococcus agalactiae cells within the biofilm, whereas both natural honeys significantly reduced the viable bacteria. Although Enterococcus faecalis was highly resistant to Def-1, Def-1 significantly affected the biofilm formation of Enterococcus faecalis and Streptococcus agalactiae after 24 h of treatment, most likely by inhibiting its extracellular polymeric substances production. In conclusion, our study revealed that honey and Def-1 are effective against established multispecies biofilm; however, Enterococcus faecalis grown in multispecies biofilm was resistant to both antimicrobials.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Sojka

Valachová

Bucekova

et al. 2016

Journal of Medical Microbiology

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“…Vancomycin concentrations well over 4000 lg/mL are readily obtained in the knee immediately after infusion, and with a daily infusion regimen, this level can be achieved daily for 6 weeks or longer [46]. Moderate concentrations of antibiotics are necessary to prevent biofilm formation [11,12], but once biofilm and persister cells have formed, concentrations of orders of magnitude greater (as high as 4000 lg/ mL) are necessary to eradicate the bacteria within the biofilm [7,8,11,12,17,27,31,45,49,50]. Clearly, some risk of toxicity exists with direct intraarticular infusion of antibiotics, but this method may be the safest way to achieve high antibiotic levels over time [54][55][56] while allowing for the opportunity to decrease the dose, change antibiotics, or stop them altogether in the case of allergic reactions or renal toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…High local antibiotic concentration around the site of an infected THA is a matter of major clinical importance, because bacteria protected by biofilm require concentrations that are orders of magnitude greater than the minimal inhibitory concentration for the planktonic forms of the same bacterium to eliminate resistant organisms that are protected by the glycocalyx [8,11,12,16,32]. Intravenous antibiotics generally do not achieve these levels of concentration in synovial fluid, but instead achieve levels around two to three times the minimal inhibitory concentration (MIC) [46].…”

Section: Introductionmentioning

confidence: 99%

One-stage Revision With Catheter Infusion of Intraarticular Antibiotics Successfully Treats Infected THA

Whiteside

Roy

2017

Clinical Orthopaedics &Amp; Related Research

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Background Two-stage revision surgery for infected total hip arthroplasty (THA) is commonly advocated, but substantial morbidity and expense are associated with this technique. In certain cases of infected THA, treatment with one-stage revision surgery and intraarticular infusion of antibiotics may offer a reasonable alternative with the distinct advantage of providing a means of delivering the drug in high concentrations. Questions/purposes We describe a protocol for intraarticular delivery of antibiotics to the hip through an indwelling catheter combined with one-stage revision surgery and examine (1) the success as judged by eradication of infection at 1 year when treating chronically infected cemented stems; (2) success in treating late-onset acute infections in well-ingrown cementless stems; and (3) what complications were associated with this approach in a small case series. Methods Between

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“…There are many kind of AMPs were found active against bacterial biofilms in that way (Dosler and Mataracı 2013;Dosler and Karaaslan 2014). However, the anti-biofilm activities of AMPs are not completely understood, there are some possible explanations provided by some limited studies.…”

Section: Amps' Mechanisms Of Actionsmentioning

confidence: 99%

Antimicrobial peptides: Coming to the end of antibiotic era, the most promising agents

Döşler¹

2017

Istanbul J Pharm

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Recently, because of the rising in multidrug resistance from infectious agents, there is a prompted interest for the development of new antimicrobial agents and new therapeutic strategies to combat the infections caused by the resistant bacteria. Among them, the natural bactericidal compounds, such as antimicrobial cationic peptides (AMPs) seems very promising agents. AMPs are the important component of the innate immune response to the surrounding microorganisms. This substances which can be isolated from most of the living organisms, have various activity like broad spectrum antibacterial, antifungal, antiviral, and antiprotozoal. However there are some resistance mechanisms that affects the AMPs, because of the rapid action and existing more than one mechanism of action, development of resistance to AMPs is quite rare. Due to their many advantages and characteristics, AMPs looks like a good candidate for being a new generation, active antimicrobial agent for antimicrobial chemotherapy against especially multi drug resistant bacteria and biofilms, either alone or in combination.

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Inhibition and destruction of Pseudomonas aeruginosa biofilms by antibiotics and antimicrobial peptides

Cited by 181 publications

References 24 publications

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

Antibiofilm efficacy of honey and bee-derived defensin-1 on multispecies wound biofilm

One-stage Revision With Catheter Infusion of Intraarticular Antibiotics Successfully Treats Infected THA

Antimicrobial peptides: Coming to the end of antibiotic era, the most promising agents

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