Inhibition Activity of Wild Berry Juice Fractions against Streptococcus pneumoniae Binding to Human Bronchial Cells

Abstract

Hematopoietic development is a delicate balance of cell fate decisions in multipotent cells between self‐renewal and differentiation. In multiple developmental systems, the Notch receptors areimportant factors regulating these processes. Hematopoietic progenitor cells have been shown to express Notch1, and studies with an activated intracellular form has revealed a functional role. To assess the function of other Notch members in hematopoiesis, we investigated the expression pattern of Notch1, Notch2, and Notch3 in hematopoietic lineages at the level of RNA and protein. We demonstrate that Notch1 and Notch2 are expressed in multiple lineages, and that Notch1 in particular appears to be regulated during myeloid differentiation. Notch1 was up‐regulated and expressed at high levels in adherent macrophages. Mast cells expressed only low levels of Notch1 mRNA whereas Notch2 mRNA was highly expressed. In addition we could detect Notch3 mRNA and protein in cell lines representing mast cell progenitors. These expression patterns imply that the different Notch genes may have very distinct functions during hematopoiesis, and that Notch3 could be a specific regulator of mast cell development. The finding that Notch1 was up‐regulated in the adherent cells developing from a multipotent progenitor cell line suggests that this protein may posses dual functions in hematopoiesis, i.e. at the stage of cell fate decision, and at the maturation stage of monocytes when adhesion to the specific microenvironment is accomplished.

Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae

“…Therefore, several studies have focused on alternative strategies to cure pneumococcal infections 22–24 . It has been known for a long time that plants are a source of antimicrobial compounds 25 .…”

Antimicrobial activity of different Finnish monofloral honeys against human pathogenic bacteria

“…S. pyogenes , S. aureus , and MRSA were cultured at 37 °C on sheep blood agar plates (Becton Dickinson, Franklin Lakes, NJ, USA). S. pneumoniae was cultured at 37 °C in CO 2 atmosphere on sheep blood agar plates (Labema Inc., Kerava, Finland) as previously described 24.…”

Antimicrobial effects of fractions from cranberry products on the growth of seven pathogenic bacteria

“…Also, there has been an increasing interest in exploring new antimicrobials of natural origins because of the potential toxicity of synthetic antimicrobials and consumers' preference (Gould, 1996). In addition, some fractions of cranberry polyphenols could present significant potential benefits for human health (Bodet, Chandad, & Grenier, 2007;Ferguson, Kurowska, Freeman, Chambers, & Koropatnick, 2004;Huttunen, Toivanen, Arkko, Ruponen, & Tikkanen-Kaukanen, 2011;Kandil et al, 2002;La, Labrecque, & Grenier, 2009;Singh et al, 2007). Thus, the aim of the present study was to evaluate the antimicrobial capabilities of fractions of different polarity obtained from two cranberry juices (clarified juice and juice concentrate) and three extracts (anthocyanins, water-soluble and apolar phenolic compounds) from cranberry fruits and pomace on food poisoning and airborne bacteria, including E. coli O157:H7 EDL 933, S. Typhimurium SL1344, L. monocytogenes HPB 2812 1/2a, Enterococcus faecium resistant to vancomycin (ERV), E. coli ATCC 25922, S. aureus ATCC 29213 and P. aeruginosa ATCC 15442.…”