Inhibiting roles of melanin-concentrating hormone for skin pigment dispersion in barfin flounder, Verasper moseri

Mizusawa, Kanta; Kobayashi, Yuki; Sunuma, Toshikazu; Asahida, Takashi; Saito, Yumiko; Takahashi, Akiyoshi

doi:10.1016/j.ygcen.2010.12.008

Cited by 42 publications

(25 citation statements)

References 29 publications

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“…1.7 ± 0.2* ,C week for 10 weeks) suppressed pigmentation of the non-eye-side color in barfin flounder , suggesting that MCH might suppress melanophore development or melanin synthesis. Continuous MCH injections (2 µg/g body weight, once per 3 days, a total of nine doses), however, failed to change the pigment cell density (Mizusawa et al 2011), suggesting that MCH might not affect chromatophore differentiation in the dorsal fin, at least under the experimental conditions employed in this study. Rather than a morphological color change, background color adaptation of the dorsal fin might be caused by a physiological color change induced by the control of pigment migration.…”

Section: Constitutive Action Of α α α α α-Msh and Flexible Action Of mentioning

confidence: 59%

“…However, the effect of long-term background adaptation on the proliferation or pigmentation of eye-side chromatophores is not known in this fish. We found that pigment dispersion of chromatophores in the dorsal skin exhibited good correlation with background color after 300 days' acclimation in black, yellow, or white tanks, while chromatophore density remained similar, regardless of tank color (Mizusawa et al 2011). These results suggest that, at least in the dorsal fin, long-term background adaptation might not yield differential chromatophore proliferation.…”

Section: Constitutive Action Of α α α α α-Msh and Flexible Action Of mentioning

confidence: 71%

“…The processes described above concerning the activities of α-MSH-rp and MCH may be part of the complex regulation of pigment migration for delicate and subtle changes in the hues and patterns of barfin flounder, in which endocrine systems uniformly affect the entire skin, while neural systems exert local control (Mizusawa et al 2011). It is conceivable that MSH and MCH are involved in various unknown biological processes because receptor genes of MSH and MCH are expressed in a wide variety of tissues of barfin flounder Kobayashi et al 2008aKobayashi et al , 2010Kobayashi et al , 2011a.…”

Section: General Discussion-biological Significance Of Melanotropic Pmentioning

confidence: 99%

“…Expression of mch and biosynthesis of MCH are modulated in response to background color (Takahashi et al 2004;Amiya et al 2005;Yamanome et al 2005). Indeed, the degree of pigment aggregation in response to different background colors seems to be proportional to the body color change in background color-adapted fish Mizusawa et al 2011). Thus, there is good agreement among background color, MCH production, pigment concentration, and body color change.…”

Section: Constitutive Action Of α α α α α-Msh and Flexible Action Of mentioning

confidence: 91%

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Multifunctional Roles of Melanocyte-Stimulating Hormone and Melanin-Concentrating Hormone in Fish: Evolution from Classical Body Color Change

Takahashi¹,

Mizusawa²,

Amano³

2014

Aqua-BioSci. Monogr. (ABSM)

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The representative role of melanocyte-stimulating hormone (MSH) and melaninconcentrating hormone (MCH) in fish is regulation of pigment migration. However, our studies using barfin flounder Verasper moseri, a flatfish as a major experimental fish, have revealed that MSH and MCH are multifunctional because their receptors are widely distributed not only in the melanophores but also in the brain and systemic body. Their biological roles other than control of pigment migration would be regulation of feeding behavior, energy metabolism, cortisol release, etc. Among them, an interesting biological process on molecular level has been observed in the role of α-MSH. A fine difference in the structure-presence or absence of one acetyl group-modified the activities. Namely, desacetyl-α-MSH having no acetyl group at N-terminal stimulates pigment dispersion in melanophore and cortisol release from the interrenal gland, while α-MSH having one acetyl group has negligible effects. On the whole body level, MCH probably transfers information about photic conditions from the external environment to the body. MCH production is changeable, depending on the difference in the intensity of the light. A white background enhances production of MCH, and MCH turns body color pale by aggregating pigments in scales. It is suggested that this peptide stimulates feeding behavior. This monograph reveals molecular characteristic and biological significance of MSH and MCH systems in fish.

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Section: Constitutive Action Of α α α α α-Msh and Flexible Action Of mentioning

confidence: 59%

Section: Constitutive Action Of α α α α α-Msh and Flexible Action Of mentioning

confidence: 71%

Section: General Discussion-biological Significance Of Melanotropic Pmentioning

confidence: 99%

Section: Constitutive Action Of α α α α α-Msh and Flexible Action Of mentioning

confidence: 91%

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Multifunctional Roles of Melanocyte-Stimulating Hormone and Melanin-Concentrating Hormone in Fish: Evolution from Classical Body Color Change

Takahashi¹,

Mizusawa²,

Amano³

2014

Aqua-BioSci. Monogr. (ABSM)

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“…Melanin protects the skin from ultraviolet radiation damage; however, excess expression or uneven distribution of melanin may cause chloasma, freckles and post-inflammatory hyperpigmentation (de Fine Olivarius et al, 1999;Hearing, 2005;Mizusawa et al, 2011). Therefore, whitening agents have been widely used in cosmetics.…”

Section: Introductionmentioning

confidence: 99%

Effect of Fucoidan on B16 Murine Melanoma Cell Melanin Formation and Apoptosis

Wang¹,

Xu²,

Liang³

et al. 2017

Afr J Tradit Complement Altern Med

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Background：Fucoidan is a complex sulfated polysaccharide extracted from brown seaweed and has a wide variety of biological activities. It not only inhibits cancer cell growth but also inhibits tyrosinase in vitro. Therefore, it is of interest to investigate the effect of fucoidan on B16 murine melanoma cells as the findings may provide new insights into the underlying mechanism regarding the inhibition of melanin formation by fucoidan. In the present study, we aimed to investigate the anti-melanogenic effect of fucoidan and its inhibitory effect on B16 cells. Materials and Methods:The influence of fucoidan on B16 melanoma cells and cellular tyrosinase was examined. Cell viability was examined by the cell counting kit-8 assay. Cellular tyrosinase activity and melanin content were determined using spectrophotometric methods and protein expression was analyzed by immunoblotting. Morphological changes in B16 melanoma cells were examined by phase contrast microscopy and apoptosis was analyzed by flow cytometry. Results: In vitro studies were performed using cell viability analysis and showed that fucoidan significantly decreased viable cell number in a dose-response manner with an IC50 of 550 ±4.3 µg/mL. Cell morphology was altered and significant apoptosis was induced when cells were exposed to 550 µg/mL fucoidan for 48 h. Conclusion: This study provides substantial evidence to show that fucoidan inhibits B16 melanoma cell proliferation and cellular tyrosinase activity. Fucoidan may be useful in the treatment of hyperpigmentation and as a skin-whitening agent in the cosmetics industry.

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Ocular‐Side Lateralization of Adult‐Type Chromatophore Precursors: Development of Pigment Asymmetry in Metamorphosing Flounder Larvae

et al. 2013

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The adult-type chromatophores of flounder differentiate at metamorphosis in the skin of ocular side to establish asymmetric pigmentation. In young larva and before metamorphosis, adult-type melanophores that migrate to the ocular side during metamorphosis reside at the base of the dorsal fin as latent precursors. However, the migration route taken by these precursor cells and the mechanisms by which lateralization and asymmetric pigmentation develop on the ocular side are unknown. To further investigate this migration and lateralization, we used in situ hybridization with gch2 probe, a marker for melanoblasts and xanthoblasts (precursors of adult type chromatophores), to examine the distribution of chromatophore precursors in metamorphosing larvae. The gch2-positive precursors were present in the myoseptum as well as in the skin. This finding indicated that these precursors migrated from the dorsal part of the fin to the skin via the myoseptum. Additionally, there were much fewer gch2-positive cells in the myoseptum of the blind side than in the skin and myoseptum of the ocular side, and this finding indicated either that migration of the precursor cells into the myoseptum of blind side was inhibited or that the precursors were eliminated from the myoseptum of the blind side. Therefore, we propose that the signals responsible for development of asymmetric pigmentation in flounder reside not only in the skin but on a larger scale and in multiple tissues throughout the lateral half of the trunk.

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Inhibiting roles of melanin-concentrating hormone for skin pigment dispersion in barfin flounder, Verasper moseri

Cited by 42 publications

References 29 publications

Multifunctional Roles of Melanocyte-Stimulating Hormone and Melanin-Concentrating Hormone in Fish: Evolution from Classical Body Color Change

Multifunctional Roles of Melanocyte-Stimulating Hormone and Melanin-Concentrating Hormone in Fish: Evolution from Classical Body Color Change

Effect of Fucoidan on B16 Murine Melanoma Cell Melanin Formation and Apoptosis

Ocular‐Side Lateralization of Adult‐Type Chromatophore Precursors: Development of Pigment Asymmetry in Metamorphosing Flounder Larvae

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