Inhibiting Effect of Zinc Oxide Nanoparticles on Advanced Glycation Products and Oxidative Modifications: a Potential Tool to Counteract Oxidative Stress in Neurodegenerative Diseases

Abstract

Advanced glycation end products (AGEs) are implicated in several central nervous system (CNS) pathologies including Alzheimer and Parkinson's diseases. In the face-off of AGE menace, we have attempted to investigate the zinc oxide nanoparticle (ZnONP) role in inhibition of AGE formation. Synthesized ZnONPs were used to investigate the inhibitory effects on AGE formation. The inhibitory effects of ZnONPs on AGE formation were determined by biophysical immunological and biochemical techniques. The results showed that ZnONP is a potential anti-glycating agent inhibiting AGE formation as well as protecting the protein structure from change. Therefore, our findings suggest ZnONPs may be used as a therapeutic in resolving the AGE role in CNS-related complications.

Biosynthesized ZnO-NPs from Morus indica Attenuates Methylglyoxal-Induced Protein Glycation and RBC Damage: In-Vitro, In-Vivo and Molecular Docking Study

“…In the present study, the co-incubation of BSA with MGO and ZnO-NPs resulted in a decrease in fluorescence, indicating the positive effect of nanoparticles in inhibition of total AGEs and argpyrimidine in a dose-dependent manner ( Figure 5 and Supplementary Table S2). The results are in agreement with our previous study, wherein ZnO-NPs synthesized from aqueous A. vera leaf extract possessed an inhibitory effect on AGE formation in a concentration-dependent manner [23]. This inhibitory activity of ZnO-NPs attributes to its carbonyl scavenging potential, and the results of the present study are in line with earlier studies on glucose-treated BSA [48].…”

“…The crystalline size of the synthesized nanoparticles calculated by Scherrer's formula was of~6-12 nm. The XRD results corroborate with the previous findings of [23] wherein ZnO-NPs were synthesized by A. vera leaf extract with a size of 42.8 nm. The FT-IR spectroscopic analysis was carried out to recognize the functional groups in plant extracts, which play a vital role in the processes of reduction and stabilization of the biosynthesis of nanoparticles [37].…”

“…Ultraviolet (UV)-Vis spectra (Beckman Coulter, DU739, Krefeld, Germany) and Powder X-Ray Diffraction (PXRD) patterns of ZnO-NPs were analyzed as reported by Ashraf et al [23] and the particle size was calculated using the Scherrer's formula:…”

“…Similarly, chemically synthesized and green synthesized ZnO nanoparticles showed the presence of high-quality ZnO-NPs when visualized by SEM and EDS [41,42]. The elemental analysis of ZnO-NPs biosynthesized using A. vera leaf extract was also carried out through EDS analysis [23].…”

Key Role of Microtubule and Its Acetylation in a Zinc Oxide Nanoparticle–Mediated Lysosome–Autophagy System

“…As one of the most widely used NMs, zinc oxide nanoparticles (ZNPs) are effective for neurodegenerative disease and cancer treatment, where autophagy is deemed to be one of its main biological effects . How ZNPs induce autophagy is poorly understood.…”