Inhibited Long Non-Coding RNA OIP5-AS1 Elevated microRNA-92a to Suppress Proliferation and Metastasis of Ovarian Cancer Cells by Silencing ITGA6

Wang, Yujue; Li, Lingling; Zhang, Xun; Zhao, Xiaolan

doi:10.21203/rs.3.rs-86230/v1

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…lncRNA can serve as ceRNA to suppress biological functions through posttranscriptional regulation. Wang et al (2022b) found that downregulation of OIP5-AS1 promoted the expression of miR-92a to repress cell proliferation and metastasis of ovarian cancer via regulating ITGA6. Zhang et al (2022a) reported that RNASEH1-AS1 exacerbated its oncogenicity of NSCLC through regulating miR-516a-5p/FOXK1 axis and facilitating Wnt/β-catenin signaling pathway activation.…”

Section: Discussionmentioning

confidence: 98%

RP11-79H23.3 Inhibits the Proliferation and Metastasis of Non-small-cell Lung Cancer Through Promoting miR-29c

Liu

et al. 2022

Biochem Genet

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Evidences indicate that long non-coding RNAs (lncRNAs) are closely involved and contributed to tumorigenesis and cancer progression. As a novel lncRNA, RP11-79H23.3 was found to be an anti-oncogene in bladder cancer. However, the essential roles and functions of RP11-79H23.3 in non-small-cell lung cancer (NSCLC) remains to be elucidated. Here, loss of functional assay was applied to gain insights into the functions of RP11-79H23.3 on the proliferation and metastasis capabilities of A549 and H1299 cells. Meantime, Real-time PCR was utilized to measure RP11-79H23.3 and miR-29c expression in NSCLC tissues. Dual-luciferase reporter assay, CCK8, colony formation assay, transwell and Western blot were performed to illustrate the potential molecular basis of RP11-79H23.3 in NSCLC. RP11-79H23.3 downregulation facilitated cell proliferation, migration, and invasion of NSCLC. The result of dual-luciferase reporter assay represented a direct interaction of RP11-79H23.3 with miR-29c, which suppressed miR-29c expression that showed inversely correlation in NSCLC. Moreover, RP11-79H23.3 siRNA facilitated the progression of NSCLC partially via regulating the expression of miR-29c and the activation of Wnt/β-catenin signaling pathway. Our findings highlighted that RP11-79H23.3, served as an anti-oncogene, accelerated NSCLC progression through sequestering miR-29c, providing a promising therapeutic target for NSCLC.

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Section: Discussionmentioning

confidence: 98%

RP11-79H23.3 Inhibits the Proliferation and Metastasis of Non-small-cell Lung Cancer Through Promoting miR-29c

Liu

et al. 2022

Biochem Genet

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“…ITGA6 has been found highly expressed in several tumors, such as bladder cancer [30], ovarian cancer [31], and colorectal cancer [32]. ITGA6 is also reported to play a role in pancreatic cancer.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Analysis on the Expression and Prognostic value of ITGA Family in Human Pancreatic Adenocarcinoma

Yang

Gao

et al. 2022

Preprint

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Background: Pancreatic adenocarcinoma (PAAD) is the most common histological subtype of pancreatic cancer. Although dramatic progress has been made in multimodal therapies, PAAD still has a poor diagnosis and prognosis. The integrin α (ITGA) family genes play a fundamental role in different cancers. However, the potential values of ITGA family members in PAAD remain elusive. This study aimed to analyze the relationship between and ITGA family genes and PAAD.Methods: A variety of bioinformatic approaches, including UALCAN, GEPIA, Human Protein Atlas (HPA) database, Kaplan-Meier Plotter, cBioPortal, and STRING, which were used to analyze the potential roles of ITGA family members in human PAAD. The results were further verified in PAAD cell lines.Results: The transcriptional levels of ITGA 1/2/3/5/6/9/11 were up-regulated, whereas the expression of ITGA 4/8/10 were not obvious in patients with PAAD compared with that in normal pancreas. And a noticeable correlation was also discovered between the over-expressions of ITGA 2/3/5/6/11 and the clinical cancer stages of PAAD patients. Survival analysis revealed that the high mRNA expression of ITGA 2/3/5/6 and 11 were associated with overall survival (OS) in PAAD patients.Conclusion: Detection of ITGA genes has values in the diagnosis and prognosis of PAAD. ITGA 2/3/5/6/11 might serve as indicators of tumor stages of PAAD, while ITGA 2/5 may be new biomarkers for evaluating the prognosis of PAAD.

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“…OIP5-AS1 increased invasion, migration and EMT via targeting miR-147a and IGF1R in cervical cancer ( 18 ). OIP5-AS1 inhibited miR-92a and increased cell growth and metastasis via upregulation of ITGA6 in ovarian cancer ( 19 ). OIP5-AS1 interacted with miR-34a and increased the PD-L1 expression in NSCLC cells ( 20 ).…”

Section: Introductionmentioning

confidence: 99%

LncRNA OIP5-AS1 Knockdown Targets miR-183-5p/GLUL Axis and Inhibits Cell Proliferation, Migration and Metastasis in Nasopharyngeal Carcinoma

Tang

Zen

et al. 2022

Front. Oncol.

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Nasopharyngeal carcinoma (NPC) is often associated with the infection of Epstein-Barr virus in nasopharynx and is mainly happened in South China and Southeast Asia. Recently, noncoding RNAs have been reported to regulate NPC carcinogenesis. LncRNA OIP5-AS1 participates in tumorigenesis and progression; however, the inherent mechanism of OIP5-AS1-mediated progression of NPC is unclear. In the current study, we aimed to explore the role of OIP5-AS1 in NPC progression. We measured the cell viability, apoptosis, migration, and invasion in NPC cells after OIP5-AS1 modulation. Moreover, we determined whether OIP5-AS1 exerts its oncogenic functions via sponging miR-183-5p in NPC. Furthermore, we determined whether glutamate ammonia ligase (GLUL) was a downstream target of miR-183-5p. We found that OIP5-AS1 downregulation inhibited the viability, migration and invasion of NPC via targeting miR-183-5p. We also identified that GLUL might be a potential downstream target of miR-183-5p in NPC cells. Mechanistically, OIP5-AS1 promotes cell motility via regulating miR-183-5p and GLUL in NPC cells. We concluded that OIP5-AS1 performed its biological functions via targeting miR-183-5p and GLUL in NPC cells.

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Inhibited Long Non-Coding RNA OIP5-AS1 Elevated microRNA-92a to Suppress Proliferation and Metastasis of Ovarian Cancer Cells by Silencing ITGA6

Cited by 5 publications

References 31 publications

RP11-79H23.3 Inhibits the Proliferation and Metastasis of Non-small-cell Lung Cancer Through Promoting miR-29c

RP11-79H23.3 Inhibits the Proliferation and Metastasis of Non-small-cell Lung Cancer Through Promoting miR-29c

Comprehensive Analysis on the Expression and Prognostic value of ITGA Family in Human Pancreatic Adenocarcinoma

LncRNA OIP5-AS1 Knockdown Targets miR-183-5p/GLUL Axis and Inhibits Cell Proliferation, Migration and Metastasis in Nasopharyngeal Carcinoma

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