Inhibin production by bovine ovarian tissues in vitro and its regulation by androgens

Abstract

stimulated granulosa cell inhibin production, methylestrenolone being the more potent. These findings provide further evidence that androgens regulate follicular inhibin and progesterone production and that these may be receptor-mediated processes, and suggest that inhibin production may be a general property of androgenic compounds.Preliminary examination of the physicochemical characteristics of inhibin indicated that the inhibin activity of bovine granulosa cell culture medium was (a) retained by an Amicon XM100A filter with a nominal molecular weight cut-off point of 100 000; and (b) destroyed by heating to 80\s=deg\Cfor 30 min.

Reproductive effects of immunizing heifers against androstenedione and oestradiol-17

“…Binding of androgens by the antibody in the follicle (follicular fluid) may help to reduce the rate of atresia and increase the pool of follicles developing to the ovulatory stage by alteration of the intraovarian concentrations of androgens. Follicular anti-androstenedione may decrease the effects of androgen on granulosa inhibin production (Henderson & Franchimont, 1983) which would subsequently allow an overall increased stimulation of follicular development by gonadotrophins. Alternatively, the presence of the antibody in follicular fluid may produce even higher concentrations of follicular steroid concentrations.…”

Inhibin production in vitro by granulosa cells from Booroola ewes which were either homozygous or non-carriers of a fecundity gene influencing their ovulation rate

“…Inhibin is therefore a possible candidate for mediating the effects of the Booroola F gene. There may be differences between the Booroola genotypes in inhibin production by granulosa cells, these cells being the principal site of ovarian inhibin produc¬ tion (Henderson & Franchimont, 1983;Mann etai, 1989; Rodgerse/a/., 1989;Torney et ai, 1989). The purpose of this study was to investigate inhibin production by granulosa cells in vitro using cells from follicles of various sizes and health obtained from Booroola Romney ewes which were homozygous (FF) or non-carriers ( ++) of the F gene.…”

Stage-Specific Distributions of Inhibin .ALPHA.-subunit in Bovine Ovarian Follicles and Various Hormones Levels in Follicular Fluid.

“…Even if the production of mature inhibin (αβ-heterodimer) is decreased, the production of monomeric inhibin α-subunit might remain unaffected, and therefore the level of monomeric inhibin α-subunit could remain high in stage III follicles. Follicular fluid T stimulates inhibin production in granulosa cells [2,3,4,35], and the T level was the lowest in stage III follicles. Inhibin production in stage III follicles is considered to be down-regulated.…”

“…nhibin is a glycoprotein with a molecular weight of 32 kDa and a heterodimer consisting of an α-subunit (20 kDa) and one of two β-subunits (βA or βB; 13 kDa) linked by disulfide bridges, and the α-and β-subunits are produced by different genes [1][2][3][4][5][6][7][8][9][10]. Inhibin is mainly produced by the granulosa cells of the ovaries, and its major physiological role is specific suppression of secretion of folliclestimulating hormone (FSH) from the anterior pituitary gland [1][2][3][4][5][6][7][8][9][10][11][12][13][14].…”

“…Inhibin is mainly produced by the granulosa cells of the ovaries, and its major physiological role is specific suppression of secretion of folliclestimulating hormone (FSH) from the anterior pituitary gland [1][2][3][4][5][6][7][8][9][10][11][12][13][14]. Many studies of inhibin activity estimated by radioimmunoassay and/or bioassay have demonstrated that growing healthy follicles produce and secrete much more inhibin into follicular fluid than atretic follicles [4,5,7,9,[15][16][17][18].…”