Inheritance of some marker genes in Setaria italica (L.) P. Beauv.

Abstract

A study on a series of genetic markers was run on five hybrids of foxtail millet, Setaria italica, and on one interspecific hybrid S. viridisxS. italica (S. viridis is the wild relative of S. italica). Seven enzymatic systems were investigated using starch gel electrophoresis (esterase, alcohol dehydrogenase, glutamate oxaloacetate transaminase, acid phosphatase, malate dehydrogenase, 6-phosphogluconate dehydrogenase, cathodic peroxidase). This genetic analysis of the 6 F2 has allowed us to define 12 polymorphic loci: Est-1, -2 and -3, Adh-1, Got-1 and -2, Acph-1, Mdh-1 and -2, Pgd-1 and -2, and Pox-1. All of them behaved like dimers, except Est-1 and Est-2 which showed monomeric structures. Two other markers were examined: waxy endosperm, which appeared to be controlled by one locus, and anthocyanic pigmentation of the collar, for which at least two loci are responsible. Studies of linkage carried out on three F2 showed two linkage groups: Mdh-1, Pox-1, Wx, Est-3, and a locus for collar colour, and Est-2, and one or two other loci of colouring.

Genetic analysis of NEKODE1 gene involved in panicle branching of foxtail millet, Setaria italica (L.) P. Beauv., and mapping by using QTL-seq

“…We designated the gene symbol for this trait of our material as NEKODE1, a dominant allele. Segregation ratio of the red stem to green stem in Cross 1 was 151:41, and it fitted the expected segregation ratio of 3:1 (v 2 = 1.68 \ v 0.05.1 2 = 3.84) and coincided with previous reports (Cherisey et al 1985;Till-Bottraud and Brabant 1990;Sato et al 2013). The segregation ratio of normal to spikelet-tipped bristles (stb) in Cross 1 was 147:44 and fitted the expected value of 3:1 (v 2 = 1.68 \ v 0.05.1 2 = 3.84) as reported by Ayyangar et al (1933) and Sato et al (2013).…”

“…We scored tip-branched panicles as ''NEKODE'' and normal types as ''normal'' after heading. We also investigated segregation of stem color (green or red; Cherisey et al 1985;Till-Bottraud and Brabant 1990) and spikelet-tipped bristles (stb: Sato et al 2013) in the F 2 progeny derived from Cross 1 and anther color (Li et al 1945;Kihara 1943) in the F 2 progeny derived from Cross 2 and investigated linkages between NEKODE and these traits.…”

Inheritance and genetic diversity of some enzymes in the sexual and diploid pool of the agamic complex of Maximae (Panicum maximum Jacq., P. infestum Anders. and P. trichocladum K.Schum.)

“…The nature of enzymes (dimeric or Yes monomeric), the number of loci, the number of alleles, but also the presence or absence of distortions of segregations, are listed . These enzymatic structures were also recorded by other authors (Kahler & Allard, 1981 ;Benito, 1983 ;Price & Kahler, 1983 ;de Cherisey et al ., 1985) in other monocotyledons (Triticum, Hordeum, Avena, Setaria) . The isozymes structure that we emphasized in P maximum corresponded well to those reviewed by .…”

Protoplast Fusion Between White and Brown Oyster Mushrooms

“…Isozymes are proteins thus they can directly reflect the alteration in the DNA sequence through changes in amino acid composition which provide an extremely useful method for evaluating genetic differences due to hybridization. Manifestations of changes due to hybridization were reflected by the combination of bands from their parents (de Charisey et al 1985). In this experiment, enzyme specific stains used are malate dehydrogenase (MDH) and acid phosphatase (ACP) which gave 3 and 2-4 bands of isozymes respectively (Fig.…”

“…2 and 3). According to de Charisey et al (1985), subcellular localization of ACP is varied in the cell with 2-4 numbers of isozymes. On the other hand, subcellular localization of MDH is at the cytosol, mitochondria, and microbodies with three numbers of isozymes (Tyson et al 1986).…”