Inheritance of seed condensed tannins and their relationship with seed-coat color and pattern genes in common bean (Phaseolus vulgaris L.)

Abstract

Condensed tannins are major flavonoid end products that affect the nutritional quality of many legume seeds. They chelate minerals and interact with proteins, thus reducing their bioavailability. Tannins also contribute to seed coat color and pigment distribution or intensity. The objective of this study was to analyze the relationship between quantitative trait loci (QTL) for seed tannin concentration in common bean and Mendelian genes for seed coat color and pattern. Three populations of recombinant inbred lines, derived from crosses between the Andean and Mesoamerican genepools were used for QTL identification and for mapping STS markers associated with seed color loci. Seed coat condensed tannins were determined with a butanol-HCl method and a total of 12 QTL were identified on separate linkage groups (LGs) in each of the populations with individual QTL explaining from 10 to 64% of the phenotypic variation for this trait. Loci on linkage groups B3 and B10 were associated with the Mendelian genes Z and Bip for partly colored seed coat pattern, while a QTL on linkage group B7 was associated with the P gene which is the primary locus for the control of color expression in beans. In conclusion, this study found that the inheritance of tannin concentration fits an oligogenic model and identifies novel putative alleles at seed coat color and pattern genes that control tannin accumulation. The results will be important for the genetic improvement of nutritionally enhanced or biofortified beans that have health promoting effects from higher polyphenolics or better iron bioavailability.

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

“…In common bean, seed size appears to be under multi-genic control, with 10 QTL found in wild x cultivated advanced backcross population analysis Blair and Izquierdo, 2012). Seed coat color genes are divided into those that control pattern and those that control tone of color (Caldas and Blair, 2009). These genes are underlayed by pro-anthocyanidin pathway genes, resulting in the accumulation of tannins and anthocyanins (Díaz et al, 2010).…”

Non-Nutritive Compounds in Fabaceae Family Seeds and the Improvement of Their Nutritional Quality by Traditional Processing – a Review

“…soybean, tepary bean, navy bean, faba bean, cowpea, bambara groundnut and pigeon pea [Benjakul et The PI isolated from different species differ by molecular weight, amino acid composition and location of the active site. In most cases, PI occurring in legumes consist of two types, the Bowman-Birk (BBI; 8 kDa) and the Kunitz trypsin family inhibitors (20)(21)(22)(23)(24) [Wati et al, 2009]. The fi rst one is a small serine proteinase inhibitor containing 71 amino acids, rich in disulfi de bonds, which has two separate protease inhibitory sites capable of inhibiting trypsin and chymotrypsin enzymes [Winiarska-Mieczan, 2007].…”

Concentrations of condensed tannins and anthocyanins in common bean seed coats

“…The tannins present in the seed coats were extracted and quantified using the method described by Caldas and Blair (2009). Briefly, 10-mg samples of bean seed coats for each recombinant inbred line from the study population were weighed in triplicate and treated twice with 2.5 mL of an aqueous solution consisting of 70% acetone and 0.1% ascorbic acid followed by one treatment of 2.5 mL diethyl ether.…”

“…Recent studies have demonstrated a quantitative heredity pattern for tannin content (Guzmán-Maldonado, Martinez, Acosta, Guevara, & Paredes, 2003) that is associated with seed colour inheritance (Caldas & Blair, 2009) suggesting the possibility of obtaining genotypes with low tannin content through selection programs (Caldas, Blair, & Restrepo, 2007). Variability in the monomers of condensed tannins is thought to be associated with different seed colours (Beninger, Hosfield, & Bassett, 1999), and the type of flavonoid structure is known to govern chelate formation and reduction of metallic ions (Mira et al, 2002).…”