Inheritance of resistance to powdery mildew in the watermelon and development of a molecular marker for selecting resistant plants

Kim, Kwang-Hwan; Ahn, Sung-Gyu; Hwang, Jang Yeon; Choi, Youngmi; Moon, Hyun Kyung; Park, Young-Hoon

doi:10.1007/s13580-013-0156-1

Cited by 17 publications

(17 citation statements)

References 13 publications

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“…For the RAPD procedure, 60 10-mer Operon primers that had showed successful PCR amplification for watermelon DNA in a previous study (Kim et al, 2013) were selected and tested following methods described previously (Je et al, 2009). For cloning and sequencing of the PCR amplicons, DNA fragments were eluted from an agarose gel using a GeneAll Gel SV kit (GeneAll, Deajeon, Korea), cloned into pGEM-T Easy Vector (Promega, Madison, WI, USA) and sequenced by the dye-termination method using an ABI3730 capillary DNA sequencer (Applied Biosystems, Foster City, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…Bulked segregant analysis (BSA) is an efficient approach to screen markers linked to a target trait, especially under circumstances where genetic mapping cannot be easily applied (Kim et al, 2013;Michelmore et al, 1991). To our knowledge, no publicly available molecular markers for MAS of rind stripe pattern in watermelon have been reported.…”

Section: Introductionmentioning

confidence: 99%

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Sequence-characterized amplified polymorphism markers for selecting rind stripe pattern in watermelon (Citrullus lanatus L.)

Kim

Han

Kang

et al. 2015

Hortic. Environ. Biotechnol.

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The inheritance of foreground stripe pattern in rind of watermelon fruits [Citrullus lanatus (Thunb.) Matsum. & Nakai] was evaluated and molecular markers for selecting the Jubilee-type (JT) stripe pattern were developed based on bulked segregant analysis (BSA). Divergence in rind pattern among F2 progeny derived from crossing Crimson-type (CT) 'Arka Manik' (AM) with JT 'TS34' (TS) indicated that stripe pattern is a quantitative trait controlled by more than one gene. The BSA of F2 plants (derived from a cross between 'AM' and 'TS') using 60 random amplified polymorphic DNA (RAPD) primers revealed a distinct RAPD band (AT14-900) polymorphic between 'AM' (CT) and 'TS' (JT). The AT14-900 sequence (925 bp) was blasted to the reference watermelon (97103) genome and high sequence similarity (97.8%) was identified on physical location of 26246077 to 26246993 bp on chromosome 6. Two expressed sequence tags (ESTs) designated 'wsbin6-10' and 'wsbin6-11' that were closely linked to AT14-900 on a genetic linkage map (developed using the F2 population derived from 'AM' x 'TS') were positioned 2,216 kb and 71 kb from AT14-900, respectively on the reference watermelon genome sequence. Marker genotyping of the F2 population showed that wsbin6-11 was tightly linked to the JT stripe pattern of 'TS' and could be a useful codominant marker for selecting this trait. In a test using 100 breeding lines, 34 of the 36 lines carrying the JT stripe pattern were homozygous for the wsbin6-11 marker (450 bp) derived from 'TS', while other lines (e.g., with no stripe or CT stripe pattern) were homozygous for the wsbin6-11 marker (420 bp) derived 'AM'. These results indicated that wsbin6-11 would be a useful marker in watermelon breeding programs aiming to select for the JT stripe pattern from other various foreground and background rind patterns.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sequence-characterized amplified polymorphism markers for selecting rind stripe pattern in watermelon (Citrullus lanatus L.)

Kim

Han

Kang

et al. 2015

Hortic. Environ. Biotechnol.

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“…The DNA of susceptible samples from the 'SBB' inbred line, resistant samples from PI 254744, and pooled DNA of resistant samples from the NILs were analyzed using the RAPD method. In total, 600 UBC primers (10-mer) were used for RAPD analysis by following previously described methods (Kim et al, 2013). PCR-amplified bands of the same size were eluted from an agarose gel using GeneClean® Turbo Kit (MP Biomedicals, Inc., USA) for cloning.…”

Section: Molecular Marker Developmentmentioning

confidence: 99%

“…The authors suggested that the tested P. xanthii race 1 that is infectious to cucumber and melon does not correspond to race 1W, which is infectious to watermelon. Kim et al (2013) identified naturally occurring powdery mildew on watermelon in a greenhouse in South Korea as race 1 of P. xanthii using melon differentials during a study of inheritance of the resistance trait in watermelon.…”

Section: Introductionmentioning

confidence: 99%

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Identification of a Causal Pathogen of Watermelon Powdery Mildew in Korea and Development of a Genetic Linkage Marker for Resistance in Watermelon (Citrullus lanatus)

Han,

Rhee,

Jang

et al. 2016

HST

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Watermelon production is often limited by powdery mildew in areas with a large daily temperature range. Development of resistant watermelon cultivars can protect against powdery mildew; however, little is known about the characteristics of its causal agents. Here, we identified the genus and race of a causal pathogen of powdery mildew in Ansung province of South Korea, and developed molecular markers for the generation of resistant watermelon cultivars. The causal pathogen was determined to be Podosphaera xanthii based on multiple sequence alignments of internal transcribed spacers (ITS) of rDNA. The physiological race was identified as 1W, and the Ansung isolate was named P. xanthii 1W-AN. Following inoculation with the identified P. xanthii 1W-AN, we found inheritance of the resistant gene fitting a single dominant Mendelian model in a segregated population ('SBA' × PI 254744). To develop molecular markers linked to fungus-resistant loci, random amplified polymorphic DNA (RAPD) was accomplished between DNA pooled from eight near-isogenic lines (NILs; BC4F6), originated from PI 254744 and susceptible 'SBB' watermelon. After sequencing bands from RAPD were identified in all eight NILs and PI254744, 42 sequence-characterized amplifiedregion (SCAR) markers were developed. Overall, 107 F2 plants derived from BC4F6 NIL-1 × 'SBB' were tested, and one SCAR marker was selected. Sequence comparison between the SCAR marker and the reference watermelon genome identified three Nco I restriction enzyme sites harboring a single nucleotide polymorphism, and codominant cleavage-amplified polymorphic site markers were subsequently developed. A CAPS marker was converted to a high-resolution melt (HRM) marker, which can discriminate C/T SNP (254PMR-HRM3). The 254PMR-HRM3 marker was evaluated in 138 F2:3 plants of a segregating population ('SBA' × PI254744) and was presumed to be 4.3 cM from the resistance locus. These results could ensure P. xanthii 1W-AN resistance in watermelon germplasm and aid watermelon cultivar development in marker-assist breeding programs.

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Genomic Resources for Disease Resistance in Watermelon

Induri,

Nimmakayala,

Reddy

2023

Compendium of Plant Genomes

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Inheritance of resistance to powdery mildew in the watermelon and development of a molecular marker for selecting resistant plants

Cited by 17 publications

References 13 publications

Sequence-characterized amplified polymorphism markers for selecting rind stripe pattern in watermelon (Citrullus lanatus L.)

Sequence-characterized amplified polymorphism markers for selecting rind stripe pattern in watermelon (Citrullus lanatus L.)

Identification of a Causal Pathogen of Watermelon Powdery Mildew in Korea and Development of a Genetic Linkage Marker for Resistance in Watermelon (Citrullus lanatus)

Genomic Resources for Disease Resistance in Watermelon

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