Inheritance of Resistance to Fenpropimorph and Terbinafine, Two Sterol Biosynthesis Inhibitors, in<i>Nectria haematococca</i>

Falandre, Alexandrine Lasseron-De

doi:10.1094/phyto-81-1432

Cited by 12 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…cucurbitae (Demakopoulou et al, 1989) and 14´10 À6 in N. haematococca var. pisi (De Falandre et al, 1991). Similar results have also been reported for the mutation rates for resistance to sterol demethylase inhibitors: 90´10 À6 for triadimenol resistance in N. haematococca var.…”

Section: Discussionsupporting

confidence: 82%

See 1 more Smart Citation

Genetic control of resistance to fenpropimorph in Ustilago maydis

Markoglou

Ziogas

1999

Plant Pathology

View full text Add to dashboard Cite

Fenpropimorph‐resistant mutants of Ustilago maydis were obtained at high frequency (30 × 10−6) after UV‐irradiation followed by selection on media containing fenpropimorph (50 μg mL−1). Genetic analysis of 30 such mutants resulted in the identification of two unlinked chromosomal loci, the U/fpm‐1 locus with two allelic genes (U/fpm‐1A and U/fpm‐1B) and the U/fpm‐2 locus. The mutant genes U/fpm‐1A and U/fpm‐2 are responsible for high resistance levels (Rf: 75–100 or 257–286 based on MICs or ED50s, respectively), while the U/fpm‐1B mutation gives only a small reduction (approximately 7–10‐fold) in fenpropimorph sensitivity. Cross‐resistance studies with other SBIs showed that the major gene (U/fpm‐1A and U/fpm‐2) mutants were cross‐resistant to the related compound fenpropidin (Rf: 15–20 or 53–66 based on MICs or ED50s values, respectively) and to tridemorph (Rf: 5 or 7.1–9.5 based on MICs or ED50s values, respectively), but not to the inhibitors of steps of ergosterol biosynthesis preceding the Δ14‐reductase. The minor gene (U/fpm‐1B) mutants also had low‐level resistance (approximately 5‐fold) to tridemorph and to fenpropidin, but in contrast with the major gene mutants they were 2–10 times more sensitive to the triazoles studied (triadimefon, triadimenol, propiconazole and flusilazole) and to the pyridine, pyrifenox. Studies of the fitness of U. maydis mutants showed that in major gene mutants, resistance was not associated with changes in growth rate in liquid culture or pathogenicity on young maize plants. The minor gene mutation reduced significantly the growth rate in liquid culture and the pathogenicity, either in homozygous or heterozygous condition in dikaryotic mycelium.

show abstract

Section: Discussionsupporting

confidence: 82%

“…cucurbitae (Demakopoulou et al 1989), N. haematococca var. pisi (De Falandre et al 1991) and Penicillium caseicolum (De Falandre et al, 1987).…”

Section: Discussionmentioning

confidence: 99%

Genetic control of resistance to fenpropimorph in Ustilago maydis

Markoglou

Ziogas

1999

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…cucurbitae (Demakopoulou et al., 1989), three genes in Nectria haematococca var. pisi (De Falandre et al., 1991), two genes located on linkage group II in Aspergillus niger (Engels et al., 1998) and four genes in U. maydis (Markoglou and Ziogas, 1999, 2000) were determined. Study of genetic control of resistance to morpholine‐type fungicides in the barley powdery mildew pathogen, E. graminis f. sp.…”

Section: Discussionmentioning

confidence: 99%

“…pisi Table 4 Eect of U/fpd-mutations for resistance to fenpropidin on pathogenicity of Ustilago maydis to corn seedlings Days after inoculation for formation of galls Twelve seedlings per cross were inoculated and the infected (or dead) seedlings were evaluated; y number of teliospores per gram dry weight of gall; c pooled standard error; 12 replications; d within columns, values followed by the same letter do not dier signi®cantly according to Dunnett's multiple range test (P 0.05); e the TD-1,2 isolate carries two unlinked chromosomal genes U/tdm-1 and U/tdm-2. (De Falandre et al, 1991), two genes located on linkage group II in Aspergillus niger and four genes in U. maydis Ziogas, 1999, 2000) were determined. Study of genetic control of resistance to morpholine-type fungicides in the barley powdery mildew pathogen, E. graminis f. sp.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Control of Resistance to the Piperidine Fungicide Fenpropidin in Ustilago maydis

Markoglou

Ziogas

2001

Journal of Phytopathology

View full text Add to dashboard Cite

Mutants of Ustilago maydis (DC.) Corda, resistant to the piperidine fungicide fenpropidin, were isolated in a mutation frequency of 3.2 × 10–5, after UV‐irradiation and selection on media containing 75 μg/ml fenpropidin. Genetic analysis with 15 such mutant isolates resulted in the identification of two unlinked chromosomal loci, U/fpd‐1 and U/fpd‐2. The U/fpd mutations are responsible for moderate resistance levels to fenpropidin (Rf: 42–56 or 15 based on effective concentration causing a 50% reduction in the growth rate (EC50) or minimal inhibitory concentration (MIC) values, respectively). Haploid strains carrying both U/fpd mutations do not exhibit higher levels of resistance to fenpropidin, indicating no additivity of gene effect between non‐allelic genes. Cross‐resistance studies with other Sterol Biosynthesis inhibitors (SBIs) showed that the U/fpd‐mutant isolates exhibited a positive cross‐resistance to the piperidine piperalin and to the related morpholine fungicides fenpropimorph and tridemorph, but not to the inhibitors of C‐14 demethylase and squalene epoxidase. Crosses between mutants carrying the U/fpd‐genes with compatible isolates carrying the U/fpm or U/tdm mutations, which have been identified in previous genetic studies for resistance to morpholine fungicides fenpropimorph and tridemorph, yielded, with the exception of U/fpd‐2 × U/fpm‐2 crosses, a large number of recombinants with wild‐type sensitivity, indicating that the mutant genes involved were not allelic. Analysis of progeny from crosses between U/fpd‐2 and U/fpm‐2 mutants yielded no recombinants with wild‐type sensitivity, but a 1 : 1 progeny segregation was observed at the MIC for the U/fpd‐2 isolates, indicating that these genes are alleles of the same locus. A study of the fitness of fenpropidin‐resistant isolates showed that the U/fpd mutations do not affect the phytopathogenic fitness‐determining characteristics such as growth in liquid culture and pathogenicity on young corn plants.

show abstract

Resistance to Sterol Demethylation Inhibitors inUstilago maydis. III. Cross-Resistance Patterns and Sterol Analyses

1996

View full text Add to dashboard Cite

Two spontaneous triadimefon‐resistant mutants of Ustilago maydis, 151ar/1 and 151ar/3, were investigated with regard to their extent of cross‐resistance and their sterol composition to elicit indications about the specificity of the present resistance mechanisms. Testing resistance to various sterol biosynthesis inhibitors and toxicants with different modes of action, it could be demonstrated that, in the mutant 151ar/1, cross‐resistance was limited to the sterol demethylation inhibitors (DMIs), whereas, in strain 151ar/3, resistance included most sterol biosynthesis inhibitors studied (DMIs, morpholines, piperidines, allylamines) as well as the unrelated compounds vinclozolin and cycloheximide. Sterol analyses showed that both mutants contained ergosterol as the main sterol component. In comparison with the sensitive reference strain, the mutant 151ar/1 had a slightly elevated content of C‐14 methyl sterols, whereas in strain 151ar/3 the amount of ergosterol was increased. Triadimefon caused an accumulation of C‐14 methyl sterols and a decrease in ergosterol content in the sensitive strain and the mutant 151ar/1, whereas the other strain 151ar/3 remained unaffected. The results indicate that several resistance mechanisms are probably operating in the two mutants.

show abstract

Inheritance of Resistance to Fenpropimorph and Terbinafine, Two Sterol Biosynthesis Inhibitors, inNectria haematococca

Cited by 12 publications

References 0 publications

Genetic control of resistance to fenpropimorph in Ustilago maydis

Genetic control of resistance to fenpropimorph in Ustilago maydis

Genetic Control of Resistance to the Piperidine Fungicide Fenpropidin in Ustilago maydis

Resistance to Sterol Demethylation Inhibitors inUstilago maydis. III. Cross-Resistance Patterns and Sterol Analyses

Contact Info

Product

Resources

About