Inheritance of increased seed tocopherol content in sunflower line IAST‐413

Abstract

With 1 figure and 1 table Abstract Sunflower seeds and oil are one of the richest natural food sources of alpha‐tocopherol (vitamin E). The sunflower line IAST‐413, with enhanced alpha‐tocopherol content, was developed by pedigree selection. The objective of this research was to study the inheritance of increased tocopherol content in seeds of IAST‐413. Plants of this line were reciprocally crossed with plants of line HA89 with wild‐type tocopherol content, and their F1, F1r, F2, F2r, BCP1 and BCP2 generations were evaluated in the field in 2006 and 2007. The F3 generation was evaluated in the field in 2007. Estimated broad‐sense heritability () was 0.78 in 2006 and 0.67 in 2007, whereas narrow‐sense heritability () was 0.70 in 2006 and 0.60 in 2007. Estimate of heritability through parent–offspring correlation was 0.67 and realized heritability was 0.64. The estimated minimum number of genes conditioning increased levels of tocopherol was 1.04 in 2006 and 1.95 in 2007. This mode of inheritance and response to selection should facilitate the introgression of the trait into elite germplasm to develop sunflower oil naturally rich in vitamin E.

Genetic basis of unstable expression of high gamma-tocopherol content in sunflower seeds

“…One population, described in García-Moreno et al [ 13 ], derived from a cross between CAS-12, with wild-type tocopherol profile mainly made up of alpha-tocopherol, and IAST-540, with high gamma-tocopherol content. The other population derived from a cross between lines IAST-413 and HA-89, both with wild-type tocopherol profile mainly made up of alpha-tocopherol, though IAST-413 is characterized by increased total tocopherol content [ 34 ].…”

Inheritance of Tocopherol Content in Sunflower Seeds in Return Crossing

“…Во ВНИИМК после четырех поколений индивидуального дизруптивного отбора были выделены линии I5 imi-1 и imi-2 с повышенным (410 и 341 мг/кг), а также линии КГ27 и ВК561 с пониженным (178 и 189 мг/кг) содержанием токоферолов в ядрах семян [11]. Del Moral L. et al сообщают о наличии, как минимум, двух генов, контролирующих увеличение содержания токоферолов в семенах [13].…”

Variability of tocopherol content in sunflower lines seeds

“…Кроме того, они также обнаружили линию IAST-522 со значительно меньшим содержанием токоферолов -около 73 мг/кг. Были предприняты попытки определения генетического контроля повышенного уровня токоферолов в семенах и сформулирована гипотеза о наличии, как минимум, двух генов, контролирующих увеличение содержания токоферолов в семенах [3].…”

“…Около 3-5 г ядер семян измельчали в пируэте и фарфоровой ступке. Из средней пробы семян отбирали навеску 100 мг в пробирку объемом 10 см 3 . Добавляли 20 мг аскорбиновой кислоты и 1 мл свежеприготовленного 2 N раствора КОН в этиловом спирте.…”