Inheritance of greenbug resistance in several forms of grain sorghum and sudangrass

Radchenko, E. E.

doi:10.1134/s1022795406010078

Cited by 8 publications

(9 citation statements)

References 2 publications

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“…Damage was assessed in sorghum accessions with the identified genes of resistance to the greenbug: Sar vashi (resistance genes Sgr1 + Sgr2), Shallu (Sgr3), Deer (Sgr4), Sorgogradskoe (Sgr5), White Durra (Sgr5 + Sgr6), and Capbam (Sgr12) (Radchenko, 2000(Radchenko, , 2006. They were divided into two differentiator groups, each containing three accessions: Deer-Sar vashi-Capbam and Shallu-Sorgogradskoe-White Durra.…”

Section: Methodsmentioning

confidence: 99%

Long-term seasonal polymorphism of the Krasnodar greenbug population for virulence to sorghum varieties carrying different resistance genes

2012

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The genetic structure of the Krasnodar population of the greenbug (Schizaphis graminum Ron dani) was analyzed in 2002 to 2010. A high level of general and seasonal polymorphism for the insect virulence to different sorghum accessions was revealed. Abiotic factors were shown to play an important role in the sea sonal variation of the frequencies of virulence phenotypes: they can alter the relative competitiveness of greenbug clones, and, therefore, changes in environmental conditions lead to differential selection in the S. graminum population.

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Section: Methodsmentioning

confidence: 99%

Long-term seasonal polymorphism of the Krasnodar greenbug population for virulence to sorghum varieties carrying different resistance genes

2012

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“…Для разработки и валидации маркера, ассоциированного с доминантным аллелем гена Rf2, исследовали 20 линий, отобранных по устойчивости к фитофагу, обыкновенной злаковой тле (Schizaphis graminum Rondani), из образцов хлебного сорго (Radchenko, 2000(Radchenko, , 2006. Все линии восстанавливают фертильность пыльцы в полевых условиях.…”

Section: материал и методыunclassified

“…Кроме того, исследовали 7 линий кафрского сорго из США, способность к восстановлению фертильности пыльцы которых ранее не изучалась. Материалом служили также стерильные линии на основе ЦМС А1 (milo) Низкорослое 81с (Украина), А-10598 и А-83 (Индия), их фертильные аналоги Низкорослое 81ф, В-10598 и В-83, гибрид F 1 Низкорослое 81с × 929-3 и устойчивые к S. graminum сестринские линии F 10 BC 2 , F 11 BC 2 , выделенные из гибридов от скрещиваний стерильной линии Низкорослое 81с с восстановителем 929-3, который защищен двумя доминантными генами устойчивости к краснодарской популяции данного фитофага (Radchenko, 2006). Список образцов представлен в таблице 1.…”

Section: материал и методыunclassified

Development and validation of CAPS-marker associated with the <i>Rf2</i> gene in sorghum (<i>Sorghum bicolor</i> (L.) Moench)

Radchenko

Alpatieva

Karabitsina

et al. 2021

Biotehnologiâ i selekciâ rastenij

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Background. The development of heterotic hybrids based on cytoplasmic male sterility (CMS) is the leading strategy in breeding sorghum (Sorghum bicolor (L.) Moench). The trait of pollen fertility restoration in forms with CMS A1 (milo), predominantly used in sorghum breeding, is determined by at least two dominant complementary genes Rf1 and Rf2, and also gene Rf5. The development of accessible molecular markers of sorghum Rf genes is highly relevant for hybrid breeding, since they can significantly accelerate the process of creating female sterile forms (A lines), sterility maintainers (B lines) and pollen fertility restorers (R lines).Material and methods. The studied material included 36 sorghum accessions from the VIR collection, which differed by the ability to restore pollen fertility in forms with A1-type CMS. The nucleotide polymorphism of 935 bp fragments of the PPR genes Sobic.002G057050, Sobic.002G054100, and Sobic.002G054200 located at the chromosome 2 was studied.Results. The fragments obtained with the use of a pair of 2459403fw and 2459403 primers were 935 bp long and included parts of three genes: Sobic.002G057050, Sobic.002G054100, Sobic.002G054200. For identifying the sequence variant Sobic.002G057050-1090 associated with the Rf2 gene, Tru9 I restrictase was chosen, which allows obtaining a 572 bp fragment unique for all the studied R lines. Such a marker was found in 10 sorghum lines from West China and Kyrgyzstan, which are widely used in breeding as fertility restorers. The fragment was found neither in three lines with sterile cytoplasm and their fertile analogues, nor in 7 accessions of kafir sorghum, which lacked functional alleles of Rf genes.Conclusions. It has been demonstrated that the marker can be used for selection and checking purity of R and B/A lines. It is also applicable for verifying hybridity of F1 seeds and analyzing hybrid populations from crosses of R lines 924-4, 928-1, 929-3, 931-1, 933-1/6, 1237-3, 1243-2, 1251, 1150-1, F10BC2 with A lines Nizkorosloe 81s, А-83 and А-10598. It may be suggested that the ability to restore pollen fertility in R lines, which lack the marker CAPS- 572, is determined by another Rf gene. The studied 935 bp fragment of Sobic.002G057050 harbours 22 SNP, therefore the development of CAPS-markers for their identification and differentiation can be promising.

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“…At the same time, differential interaction with the phytophage was not detected only for the accessions protected by the Sgr7 -Sgr11 genes, i.e., the need for a further search for sorghum genotypes possessing new resistance genes is quite obvious. It has also been shown that the gene pool of the genus is characterized by a wide distribution of weakly expressed resistance genes (Radchenko, 2000(Radchenko, , 2006Radchenko and Zubov, 2007).…”

Section: Geneticsmentioning

confidence: 99%

The ideas of N.I. Vavilov and current problems of crop genetics

Anisimova

Radchenko

2020

BioComm

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Some results of the studies of plant genetic resources held in the VIR Department of Genetics are discussed in light of N. I. Vavilov’s theories of natural plant immunity, homologous series in hereditary variability, and interspecific hybridization. The long-term investigations resulted in identifying bread wheat, cultivated barley, oat, rye and sorghum accessions with new effective genes for resistance to diseases and pests. The large-scale immunological screening has revealed a narrow genetic diversity in wheat and barley for resistance to biotic and abiotic stressors. On the example of sunflowers, the significance of seed storage proteins for solving problems of domestication and introgressive hybridizations is demonstrated.

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Inheritance of greenbug resistance in several forms of grain sorghum and sudangrass

Cited by 8 publications

References 2 publications

Long-term seasonal polymorphism of the Krasnodar greenbug population for virulence to sorghum varieties carrying different resistance genes

Long-term seasonal polymorphism of the Krasnodar greenbug population for virulence to sorghum varieties carrying different resistance genes

Development and validation of CAPS-marker associated with the <i>Rf2</i> gene in sorghum (<i>Sorghum bicolor</i> (L.) Moench)

The ideas of N.I. Vavilov and current problems of crop genetics

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