Inheritance of C<sub>4</sub> Enzymes Associated with Carbon Fixation in <i>Flaveria</i> Species

Cameron, Randall G.; Bassett, Carole L.

doi:10.1104/pp.88.3.532

Cited by 9 publications

(16 citation statements)

References 9 publications

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“…No silver-stained band corresponding to PPDK could be detected in F. pringlei, while all other samples (except F. trinervia x F. brownii) have a single band in the PPDK position (10), although apparently at a slightly lower mol wt in F. brownii.…”

Section: Electrophoresis and Immunoblotting Of Soluble Proteinsmentioning

confidence: 93%

“…Only one band is present in F. linearis, at approximately 66 kD (10). F. brownii has a heavily stained NADP-ME band at the same position as in F. linearis, slightly higher in mol wt than the heavily stained band in F. trinervia, although NADP-ME activity was nearly identical in both species (Fig.…”

Section: Electrophoresis and Immunoblotting Of Soluble Proteinsmentioning

confidence: 94%

“…Immunostaining of polypeptides transferred to nitroceilulose consistently indicated one band corresponding to PEPC in F. trinervia (100 kD) and two PEPC bands in F. brownii, F. linearis, and all hybrids except where F. pringlei was a parent ( Fig. 2A) (10). Although no PEPC band is detectable in the extracts from F. pringlei in Figure 2A, (Fig.…”

Section: Co2 Exchangementioning

confidence: 99%

“…The polypeptides were transferred to nitrocellulose filters and the subunits corresponding to PEPC and NADP-ME were visualized by probing each filter with polyclonal antibody against either PEPC (provided by S. Sun, University of Hawaii) or NADP-ME (provided by T. Nelson, Yale University) as previously described (10). No bands below either PEPC or NADP-ME were observed in these immunoblots, indicating that the samples were relatively undegraded.…”

Section: Electrophoretic Transfer Of Polypeptides and Immunoblottingmentioning

confidence: 99%

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Transfer of C₄ Photosynthetic Characters through Hybridization of Flaveria Species

Cameron

Bassett²,

Bouton³

et al. 1989

Plant Physiol.

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Transfer of C4 photosynthetic traits was studied through hybridization of Flaverla trinervia (Spreng.) Mohr (C4) and Flaverla brownie A.M. Powell (C4-like) with Flaverla linearis Lag. (C3-C4) and the C3 species Flaverla pringlei Gandoger (C3). Fertility was low, based on irregular chromosome pairing and low pollen stainability, except in F. brownil x F. Ilnearis which had bivalent pairing and 76% stainable pollen. Hybrids had apparent photosynthesis values of 71 to 148% of the midparental means, while the CO2 compensation concentration was similar to the C4 or C4-like parent, except in hybrids having the C3 species F. pringlei as a parent. Inhibition of apparent photosynthesis by 02, and phosphoenolpyruvate carboxylase and NADP-malic enzyme activities and subunit levels in the hybrids were closer to the C3 or C3-C4 parent. The species F. brownie and F. trlnervla were equal in their capacity to transfer reduced 02 inhibition of AP and CO2 compensation concentration values to hybrids with F. Ilnearls (C3-C4), although hybrids with F. trlnervla had higher PEPC activity. The 02 inhibition of AP was correlated with the logarithm of activities of phosphoenolpyruvate carboxylase (r = -0.95) and NADP-malic enzyme (r = -0.87). These results confirm that C4 traits can be transferred by hybridization of C3-C4 and C4 or C4-like species, with a higher degree of C4 photosynthesis than exists in C3-C4 species, and at least in F. brownie x F. Ilnearis, fertile progeny are obtained.The characters associated with C4 photosynthesis provide these plants with a selective advantage in certain environments (22,27,28). The C02-concentrating mechanism of this pathway is believed to allow ribulose bisphosphate carboxylase/oxygenase to operate at Vmax and reduce its inhibition by 02, resulting in an increased photosynthetic capacity (22,23). The evolutionary origin of C4 photosynthesis appears to have been polyphyletic, having arisen several times within both monocots and dicots (26,28). An individual genus usually contains only C3 or fully evolved C4 species. This has hindered investigations of the evolution and genetic control of C4 characters, since hybrids between photosynthetic types usually are not possible, and the few that have been made reproduce poorly (6,9,14,15).

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Section: Electrophoresis and Immunoblotting Of Soluble Proteinsmentioning

confidence: 93%

Section: Electrophoresis and Immunoblotting Of Soluble Proteinsmentioning

confidence: 94%

Section: Co2 Exchangementioning

confidence: 99%

Section: Electrophoretic Transfer Of Polypeptides and Immunoblottingmentioning

confidence: 99%

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Transfer of C₄ Photosynthetic Characters through Hybridization of Flaveria Species

Cameron

Bassett²,

Bouton³

et al. 1989

Plant Physiol.

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“…While the Flaveria experiments provided some insight into whether aspects of C 4 were dominant or recessive in F1 hybrids, the sterility of the hybrids precluded quantitative trait loci analysis for C 4 traits (Brown et al, 1986(Brown et al, , 1993Cameron and Bassett, 1988;Holaday et al, 1988;Cameron et al, 1989). Similarly, the oat-maize addition lines provided insight into certain aspects of C 4 regulation but failed to reveal global regulators of the pathway.…”

Section: Geneticsmentioning

confidence: 99%

C₄ Cycles: Past, Present, and Future Research on C₄ Photosynthesis

2011

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In the late 1960s, a vibrant new research field was ignited by the discovery that instead of fixing CO 2 into a C 3 compound, some plants initially fix CO 2 into a four-carbon (C 4 ) compound. The term C 4 photosynthesis was born. In the 20 years that followed, physiologists, biochemists, and molecular and developmental biologists grappled to understand how the C 4 photosynthetic pathway was partitioned between two morphologically distinct cell types in the leaf. By the early 1990s, much was known about C 4 biochemistry, the types of leaf anatomy that facilitated the pathway, and the patterns of gene expression that underpinned the biochemistry. However, virtually nothing was known about how the pathway was regulated. It should have been an exciting time, but many of the original researchers were approaching retirement, C 4 plants were proving recalcitrant to genetic manipulation, and whole-genome sequences were not even a dream. In combination, these factors led to reduced funding and the failure to attract young people into the field; the endgame seemed to be underway. But over the last 5 years, there has been a resurgence of interest and funding, not least because of ambitious multinational projects that aim to increase crop yields by introducing C 4 traits into C 3 plants. Combined with new technologies, this renewed interest has resulted in the development of more sophisticated approaches toward understanding how the C 4 pathway evolved, how it is regulated, and how it might be manipulated. The extent of this resurgence is manifest by the publication in 2011 of more than 650 pages of reviews on different aspects of C 4 . Here, I provide an overview of our current understanding, the questions that are being addressed, and the issues that lie ahead.

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Isolation and characterization of cDNA clones for NADP-malic enzyme from leaves ofFlaveria: transcript abundance distinguishes C3, C3?C4 and C4 photosynthetic types

1991

Plant Mol Biol

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To study the control of enhanced synthesis of enzymes associated with C4 photosynthesis relative to non-C4 plants, we investigated the expression of NADP-malic enzyme (NADP-ME) in different photosynthetic types of Flaveria. Complementary DNA clones encoding NADP-ME were constructed using poly(A)+ RNA from leaves of Flaveria trinervia (C4) and F. linearis (C3-C4) and identified by homology to a cDNA clone (500 bp) encoding NADP-ME from maize (Zea mays L. [39]). The sequence of one clone from each species was determined. The Flaveria clones were 90% homologous over a 564 nucleotide region encoding the carboxy terminal end of the derived polypeptide; sequence similarity to the maize transcript in this region was 71%. Both Flaveria clones detected a 2/3 kb transcript by hybridization to poly(A)+ RNA from expanding leaves of F. trinervia, F. linearis and F. pringlei (C3). The level of transcripts paralleled previously observed NADP-ME activity and abundance differences determined in these species, suggesting that control of the expression of NADP-ME in different photosynthetic types is predominantly at the transcriptional/post-transcriptional level. Southern analysis of genomic DNAs from F. trinervia, F. linearis and F. pringlei indicated a low copy number for this gene in all three species.

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Inheritance of C₄ Enzymes Associated with Carbon Fixation in Flaveria Species

Cited by 9 publications

References 9 publications

Transfer of C₄ Photosynthetic Characters through Hybridization of Flaveria Species

Transfer of C₄ Photosynthetic Characters through Hybridization of Flaveria Species

C₄ Cycles: Past, Present, and Future Research on C₄ Photosynthesis

Isolation and characterization of cDNA clones for NADP-malic enzyme from leaves ofFlaveria: transcript abundance distinguishes C3, C3?C4 and C4 photosynthetic types

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Inheritance of C4 Enzymes Associated with Carbon Fixation in Flaveria Species

Cited by 9 publications

References 9 publications

Transfer of C4 Photosynthetic Characters through Hybridization of Flaveria Species

Transfer of C4 Photosynthetic Characters through Hybridization of Flaveria Species

C4 Cycles: Past, Present, and Future Research on C4 Photosynthesis

Isolation and characterization of cDNA clones for NADP-malic enzyme from leaves ofFlaveria: transcript abundance distinguishes C3, C3?C4 and C4 photosynthetic types

Contact Info

Product

Resources

About

Inheritance of C₄ Enzymes Associated with Carbon Fixation in Flaveria Species

Transfer of C₄ Photosynthetic Characters through Hybridization of Flaveria Species

Transfer of C₄ Photosynthetic Characters through Hybridization of Flaveria Species

C₄ Cycles: Past, Present, and Future Research on C₄ Photosynthesis