Inheritance of and Molecular Markers for Susceptibility of Malus domestica to Fruit Ring Rot (Botryosphaeria dothidea)

Abstract

To analyse the inheritance of fruit ring rot (FRR) resistance and to screen for microsatellite markers linked to resistance⁄susceptibility, 875 apple hybrid seedlings (Malus domestica, Jonathan · Golden Delicious) were inoculated with five isolates of Botryosphaeria dothidea in 2 years (2008 and 2009). The results indicated that incidence and non-incidence were qualitatively segregated, and incidence was dominant to non-incidence. The variation in susceptibility within this population was attributed to the segregation of three major genes. For the phenotype of incidence, the severity of lesion development was a quantitative trait. From 230 published microsatellite primer pairs, six markers were identified that were linked to the susceptibility to FRR. located in LG12 and LG2, respectively, were linked to susceptibility to the pathogen isolate Mx1, and their map distances to the susceptibility loci were 8.2 and 5.1 centimorgan (cM), respectively. CH01e01-120 and CH02c02b-100, which were linked to susceptibility to Ls1, were located in LG14 and LG4, and the map distances to the susceptibility loci were 16.9 and 8.4 cM, respectively. CH05d11-150 and CH03a03-230, linked to susceptibility to Lw048, were located in LG12 and LG14; for both of them, the map distance was 13.4 cM.

Selection and Evaluation of Interspecific Hybrids of Pear Highly Resistant to Venturia nashicola

“…Such observations are very consistent with those of Ishii and the colleagues, who reported the pear scab resistance as a quantitative trait (Ishii et al. 1992a), and are similar to the resistances to apple ring rot ( Botryosphaeria dothidea ) (Zhuang et al. 2011).…”

Role of lenticels and microcracks on susceptibility of apple fruit to Botryosphaeria dothidea

“…Ls1, a highly virulent B. dothidea isolate, was used for the inoculation experiments (Zhuang et al 2011). One week before inoculation, the pathogens were cultured on PDA media at 28°C according to the protocols described by Zhang et al (2010) and Zhuang et al (2011).…”

“…One week before inoculation, the pathogens were cultured on PDA media at 28°C according to the protocols described by Zhang et al (2010) and Zhuang et al (2011). Inoculations were performed on nonwounded fruit (Zhang et al 2010) with mycelial discs (7 mm diameter) that were cut with a cork borer from the edges of actively growing cultures.…”

Genetic diversity and apple leaf spot disease resistance characterization assessed by SSR markers

“…Among the molecular ones, microsatellite markers (SSR-simple sequence repeats) are highly polymorphic, multiallelic, co-dominant, reproducible and are distributed throughout the entire genome, making them ideal for revealing genetic diversity (Morgante et al 2002). Therefore, the use of molecular markers to access the apple germplasm is a trustworthy tool (Gustavsson et al 2008, Zhuang et al 2011, Potts et al 2012, Reim et al 2013, Burak et al 2014. Almost 400 microsatellite markers have already been identified and mapped in Malus and other fruit species (Guilford et al 1997, Liebhard et al 2002, Silfverberg-Dilworth et al 2006, Han and Korban 2008.…”

“…Almost 400 microsatellite markers have already been identified and mapped in Malus and other fruit species (Guilford et al 1997, Liebhard et al 2002, Silfverberg-Dilworth et al 2006, Han and Korban 2008. These molecular markers have been successfully used for assessing the gene pool and relatedness among distinct Malus germplasm (Pereira-Lorenzo et al 2008, Gasi et al 2010; and also for identifying loci associated with target alleles and for map-based cloning, regarding their co-dominant heritage and high polymorphism level (Hokanson et al 1998, Liebhard et al 2002, Oraguzie et al 2005, Naik et al 2006, Han and Korban 2010, Zhuang et al 2011). …”