Inheritance of alleles for Cgy1 and Gy4 storage protein genes in soybean

Abstract

A cultivar lacking the glycinin subunit A5A4B3 ('Raiden') was crossed with one lacking the α'-subunit of β-conglycinin ('Keburi'). Analysis of F2 and F3 progeny indicated that the missing bands of the A5A4B3 and the α'-subunit were each controlled by a recessive allele of two independently segregating genes. Gene symbols Gy 4/gy 4 and Cgy 1/cgy 1 were proposed for the genes which confer the presence or absence of the glycinin and conglycinin subunits, respectively.

Inheritance and Organization of Glycinin Genes in Soybean

“…The x 2 from this goodness-of-fit test was 0.545 (0.25 < P < 0.50). This result was consistent with previously reported data that showed these alleles resided at a single genetic locus (Kitamura et al, 1984).…”

“…The fourth genetic locus contained the alternate alleles Gy 4 and gy 4 , the latter being recessive to the former (Kitamura et al, 1984). The two alleles have been characterized extensively, and differ from one another by only two nucleotides (Scallon et al, 1987).…”

“…Despite a careful search, an RFLP was not located that permitted distinction between the two alleles. However, because the G4 glycinin subunit encoded by this gene was absent in Raiden (gy 4 j gy 4 ) (Staswick et al, 1983;Kitamura et al, 1984) and present in Forrest (Gy.,/-), these two alleles were distinguished from one another by SDS-polyacrylamide electrophoresis (Fontes et al, 1984).…”

“…A picture of a representative gel is shown in Figure 6 conditioned the simultaneous loss of the A 5 , A 4 , and B 3 peptides from the seed (Staswick et al, 1983;Kitamura et al, 1984). To obtain maximum separation of the three peptides, urea was included in the gels (Fontes et al, 1984).…”

“…The chips were cut into small pieces and added to 0.5 ml of a sample buffer consisting of 0.05 M Tris-HCI, pH 8, 0.2% SDS, and 5 M urea. After 30 min at room temperature, the extracts were analyzed by SDS-polyacrylamide gel electrophoresis as described by Kitamura et al (1984). The system contained a 5% stacking gel (2.5 cm) and a 7% to 13% running gel (7.5 cm).…”