Axillary bud proliferation of Zinnia elegans Jacq . was accomplished from vegetative bud explants of greenhouse-grown plants . Photoperiod extension via metalarc lamps in the greenhouse stock plant environment significantly increased both the quantity and quality of explants . Shoot cultures initiated from axillary buds of identified male sterile plants proliferated best on a medium with 1 gM BAP . Microshoots exhibited 80% rooting on media without growth regulators, and 100% acclimatization of rooted plantlets was accomplished in a lighted, high humidity chamber . In vitro-produced male sterile plants were more compact, more heavily branched, and demonstrated earlier flowering than seed-propagated plants . These male steriles were cross pollinated to male fertile lines to verify their ability to serve as a uniform female parent .