Inheritance and Use of Golden Crown Virescence in Cotton

Duncan, E. N.; Pate, James B.

doi:10.1093/oxfordjournals.jhered.a107598

Cited by 6 publications

(4 citation statements)

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“…Upon transferring the virescent gene into the commercial cultivars by means of backcrossing, Hua et al (1963) developed parental virescents in order to produce interspecific hybrids between Gossypium hirsutum and G. barbadense in China. Duncan and Pate (1967) and our laboratory conducted a vast amount of research into hybrid seed production using indicative virescent characteristics. The homozygous virescent plants could be easily identified and eliminated during the ordinary practices of thinning and weeding from the F 1 hybrids.…”

Section: Introductionmentioning

confidence: 99%

Rapid mapping and cloning of the virescent-1 gene in cotton by bulked segregant analysis–next generation sequencing and virus-induced gene silencing strategies

Zhu

Chen

Gao

et al. 2017

Journal of Experimental Botany

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Section: Introductionmentioning

confidence: 99%

Rapid mapping and cloning of the virescent-1 gene in cotton by bulked segregant analysis–next generation sequencing and virus-induced gene silencing strategies

Zhu

Chen

Gao

et al. 2017

Journal of Experimental Botany

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“…In addition, many important genes were subdivided into different linkage groups through T582 (Percival and Kohel 1974;Endrizzi et al 1984). Especially, seven of the 20 virescent genes were reported at one recessive locus in the tetraploid cotton species, which further have been mapped by linkage analysis (Duncan and Pate 1967;Endrizzi et al 1984). Genes v 1 and v 7 were found to be homoeoallelic, which are functionally similar and located on homoeologous chromosomes (Turcotte and Feaste 1973).…”

Section: Discussionmentioning

confidence: 99%

“…However, more than 30 virescent mutants were not found their target genes in cotton (Song et al 2012). The virescent gene v 1 can serve as a valuable resource for heterosis utilization (Duncan and Pate 1967;Ma et al 2013), for exploring the mechanism of photosynthesis as well as for a better understanding of genetic interactions.…”

Section: Discussionmentioning

confidence: 99%

“…The virescent trait with a photoperiod-sensitive genetic male sterility in cotton can reduce the cost of hybrid breeding and promote the exploitation of heterosis to overcome the limitations of seed production in the 3-line system (Zhao et al 2000;Duncan and Pate 1967;Ma et al 2013). To date, out of twenty virescent genes, seven single recessive nuclear genes from more than 30 mutants of the tetraploid cotton have been mapped in genetic linkage groups (Endrizzi et al 1984).…”

Section: Introductionmentioning

confidence: 99%

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Map-based cloning of a recessive gene v1 for virescent leaf expression in cotton (Gossypium spp.)

et al. 2018

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Background: Virescence, as a recognizable phenotype in the early development stage of cotton, is not only available for research on chloroplast development and photosynthesis but also for heterosis exploitation in cotton. Methods: In current study, for fine mapping of virescent-1 (v 1 ) in cotton, three populations with a total of 5 678 individuals were constructed using T582 which has the virescent trait. Tobacco rattle virus, TRV1 and TRV2 (pYL156), were used as vectors for the virus-induced gene silencing (VIGS) assay. Results:The v 1 gene was fine-mapped to a 20 kb interval on chromosome 20 of tetraploid cotton. We identified only one candidate gene with four single nucleotide polymorphisms between parents, among which the single nucleotide polymorphism at the position of 1 082 base pair caused the change of amino acid residue from Arg (3-79) to Lys (T582). The relative expression of the candidate gene in virescent plants was extensively lower than that in normal plants. Nullification of the gene by VIGS significantly turned the green leaf of normal cotton plants into yellow. We named this candidate gene as GhRVL. Conclusions: This study will facilitate the further research on virescent formation, and will be useful for breeding of hybrid cottons.

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Fine mapping and candidate gene analysis of the virescent gene v 1 in Upland cotton (Gossypium hirsutum)

Mao

Wei

et al. 2017

Mol Genet Genomics

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The young leaves of virescent mutants are yellowish and gradually turn green as the plants reach maturity. Understanding the genetic basis of virescent mutants can aid research of the regulatory mechanisms underlying chloroplast development and chlorophyll biosynthesis, as well as contribute to the application of virescent traits in crop breeding. In this study, fine mapping was employed, and a recessive gene (v ) from a virescent mutant of Upland cotton was narrowed to an 84.1-Kb region containing ten candidate genes. The GhChlI gene encodes the cotton Mg-chelatase I subunit (CHLI) and was identified as the candidate gene for the virescent mutation using gene annotation. BLAST analysis showed that the GhChlI gene has two copies, Gh_A10G0282 and Gh_D10G0283. Sequence analysis indicated that the coding region (CDS) of GhChlI is 1269 bp in length, with three predicted exons and one non-synonymous nucleotide mutation (G1082A) in the third exon of Gh_D10G0283, with an amino acid (AA) substitution of arginine (R) to lysine (K). GhChlI-silenced TM-1 plants exhibited a lower GhChlI expression level, a lower chlorophyll content, and the virescent phenotype. Analysis of upstream regulatory elements and expression levels of GhChlI showed that the expression quantity of GhChlI may be normal, and with the development of the true leaf, the increase in the Gh_A10G0282 dosage may partially make up for the deficiency of Gh_D10G0283 in the v mutant. Phylogenetic analysis and sequence alignment revealed that the protein sequence encoded by the third exon of GhChlI is highly conserved across diverse plant species, in which AA substitutions among the completely conserved residues frequently result in changes in leaf color in various species. These results suggest that the mutation (G1082A) within the GhChlI gene may cause a functional defect of the GhCHLI subunit and thus the virescent phenotype in the v mutant. The GhChlI mutation not only provides a tool for understanding the associations of CHLI protein function and the chlorophyll biosynthesis pathway but also has implications for cotton breeding.

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Inheritance and Use of Golden Crown Virescence in Cotton

Cited by 6 publications

References 0 publications

Rapid mapping and cloning of the virescent-1 gene in cotton by bulked segregant analysis–next generation sequencing and virus-induced gene silencing strategies

Rapid mapping and cloning of the virescent-1 gene in cotton by bulked segregant analysis–next generation sequencing and virus-induced gene silencing strategies

Map-based cloning of a recessive gene v1 for virescent leaf expression in cotton (Gossypium spp.)

Fine mapping and candidate gene analysis of the virescent gene v 1 in Upland cotton (Gossypium hirsutum)

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