Inheritance and identification of molecular markers associated with a novel dwarfing gene in barley

Abstract

BackgroundDwarfing genes have widely been used in barley breeding program. More than 30 types of dwarfs or semidwarfs have been reported, but a few has been exploited in barley breeding because pleiotropic effects of dwarfing genes cause some undesired traits. The plant architecture of newly discovered dwarfing germplasm "Huaai 11" consisted of desirable agronomic traits such as shortened stature and early maturity. Genetic factor controlling the plant height in dwarf line Huaai 11 was investigated.ResultsThe Huaai 11 was crossed with tall varieties Monker, Mpyt, Zhenongda 3, Zaoshu 3, Advance, Huadamai 1, Huadamai 6, Hyproly and Ris01508. All the F1 plants displayed tall trait. Both tall and dwarf plants appeared in all the F2 populations with a 3:1 segregation ratio, suggesting that dwarfism of Huaai 11 is controlled by a single recessive gene, btwd1. Allelism test indicated that this dwarfing gene in the Huaai 11 is nonallelic with the gene br, uzu, sdw1 and denso. Using a double haploid population derived from a cross of Huadamai 6 and Huaai 11 and SSR markers the novel dwarfing gene was mapped onto the long arm of chromosome 7H, and closely linked to Bmac031 and Bmac167 with genetic distance of 2.2 cM.ConclusionHuaai 11 is a new source of dwarf for broadening the genetic base of dwarfism. This dwarf source was controlled by a recessive dwarfing gene btwd1, was mapped onto the long arm of chromosome 7H.

Molecular detection of QTL controlling plant height components in a doubled haploid barley population

“…More than 30 types of dwarfing or semi-dwarfing genes have been found, including ari, br, cud, ert, lzd, mnd, nld, sid, sld, dwf, uzu, denso, sdw1, bdwd1, etc. (Sears et al, 1981;Franckowiak, 1987;Franckowiak and Pecio, 1992;Zhang and Zhang, 2003;Ren et al, 2010). Among these dwarfing genes, uzu has been widely used in barley breeding in China, Japan and Korea peninsula (Hoskins and Poehlman, 1971;Tsuchiya, 1976;Zhang, 1994Zhang, , 2000Saisho et al, 2004;Ren et al, 2010).…”

“…(Sears et al, 1981;Franckowiak, 1987;Franckowiak and Pecio, 1992;Zhang and Zhang, 2003;Ren et al, 2010). Among these dwarfing genes, uzu has been widely used in barley breeding in China, Japan and Korea peninsula (Hoskins and Poehlman, 1971;Tsuchiya, 1976;Zhang, 1994Zhang, , 2000Saisho et al, 2004;Ren et al, 2010). sdw1 has been verified in feed barley breeding in North America and Australia (Gymer, 1993;Hellewell et al, 2000;Jia et al, 2009;Ren et al, 2010).…”

“…sdw1 has been verified in feed barley breeding in North America and Australia (Gymer, 1993;Hellewell et al, 2000;Jia et al, 2009;Ren et al, 2010). denso has been successfully used in European barley breeding (Zhang and Zhang, 2003;Jia et al, 2009;Ren et al, 2010). In addition to the semi-dwarfing and dwarfing genes, a number of quantitative trait loci (QTL) are also known to affect plant height in barley.…”

“…Among these dwarfing genes, uzu has been widely used in barley breeding in China, Japan and Korea peninsula (Hoskins and Poehlman, 1971;Tsuchiya, 1976;Zhang, 1994Zhang, , 2000Saisho et al, 2004;Ren et al, 2010). sdw1 has been verified in feed barley breeding in North America and Australia (Gymer, 1993;Hellewell et al, 2000;Jia et al, 2009;Ren et al, 2010). denso has been successfully used in European barley breeding (Zhang and Zhang, 2003;Jia et al, 2009;Ren et al, 2010).…”

“…Huaai 11 is a new source of dwarf discovered by our research group for broadening the genetic base of dwarfism. This dwarf trait was controlled by a recessive dwarfing gene btwd1, and mapped on the long arm of chromosome 7H (Ren et al, 2010). To use this new germplasm Huaai 11 efficiently in barley-breeding programs, it is important to characterize the QTL controlling plant height components in this line.…”