Inheritance and fine mapping of a restorer-of-fertility (Rf) gene for the cytoplasmic male sterility in soybean

Dong, Dekun; Li, Z.; Yuan, Fengjie; Zhu, Shenlong; Chen, P.; Yu, Weiwei; Yang, Qinghua; Fu, Xujun; Yu, Xinying; Li, B.Q.; Zhu, Derui

doi:10.1016/j.plantsci.2012.02.007

Cited by 21 publications

(23 citation statements)

References 11 publications

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“…It is a striking point that a dominant Rf ‐gene restoring ZD‐CMS was mapped on chromosome 16 in soybean (Dong et al, ), and the same genomic region was previously reported responsible for restoring fertility to RN‐CMS (Zhao et al, ). An assumption was that a shared genomic region between two gametophytically restored CMSs (RN and ZD) hints either a common CMS‐inducing cytoplasm or a similar mechanism explaining (a solo rf ‐gene) the fertility restoration (Dong et al, ). The possible mechanism of a common rf ‐gene restoring multiple CMS in soybean is similar to what has been found in wheat (Tsunewaki, ), sorghum (Jordan et al, ; Praveen et al, ) and cotton (Zhang & Stewart, ).…”

Section: Inheritance Of Male Sterility and Fertility Restoration Systmentioning

confidence: 72%

“…Two independent rf ‐genes loci, Satt626 on linkage group (LG) “M” and Satt300 on LG “A1”, were identified that is assumed to be related to male fertility restoration of NJCMS1A (Yang et al, ). Moreover, Dong et al () observed that rf ‐genes loci, Satt135 on LG “D2”, might be linked to male fertility restoration of NJCMS2A, where the genetic distance between rf ‐gene and Satt135 in NJCMS2A was 11.47 cM. Zhao and Gai () reported that the MS of NJCMS3A and its restoration were regulated by one pair of MS genes, and Satt477, Satt331 and CSSRl33 on LG “O” were linked to the male fertility rf ‐gene of NJCMS3A with genetic distances of 8.1–10.4, 11.4–16.4 and 13.3–19.2 cM, respectively (Li, Zhao, & Gai, ).…”

Section: Genetic Mechanism Of Male Sterility In Soybeanmentioning

confidence: 99%

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Male sterility in soybean: Occurrence, molecular basis and utilization

Nadeem

Sun

et al. 2019

Plant Breeding

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In plants, male sterility (MS) is a specific breeding target trait. With the advancements in agriculture, utilization of heterosis breeding in hybrid production through MS lines has become the main breeding tool of various cross‐pollinated and even self‐pollinated crops. Soybean is an essential source of oil and protein; however, the low yield is a major factor limiting its development. Soybean MS mainly comprises cytoplasmic‐nuclear MS and nuclear/genic MS (NMS/GMS), which can effectively utilize heterosis to improve soybean yield. This review outlines the recent research progress on the development of new genetically MS lines, exploring the underlying molecular mechanism of MS, identification and cloning of MS and fertility restoration genes, and the application of MS lines. We further discussed and prospected the future developmental scenario direction of the soybean MS, based on the previous studies of other crops sterility system. Moreover, this review also provides comprehensive information for better application of MS to soybean breeding programme.

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Section: Inheritance Of Male Sterility and Fertility Restoration Systmentioning

confidence: 72%

Section: Genetic Mechanism Of Male Sterility In Soybeanmentioning

confidence: 99%

Male sterility in soybean: Occurrence, molecular basis and utilization

Nadeem

Sun

et al. 2019

Plant Breeding

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“…The CMS phenotype is a maternally inherited trait caused by incompatibility between the nuclear and cytoplasmic genomes (Ruiz & Daniell, ). Classical genetic studies have reported that CMS is associated with aberrant recombination in the mitochondrial genome (Conde, Pring, Schertz, & Ross, ; Das et al., ; Eckardt, ; Hanson & Bentolila, ; Lee, Muthukrishnan, Sorensen, & Liang, ; Levings & Pring, ; Luo et al., ; Pring, Conde, & Schertz, ; Smith & Chowdhury, ) and fertility was restored by nuclear‐encoded fertility restorer ( Rf ) genes in a number of crop species like rice, soybean, maize, sunflower (Ahmadikhah & Karlov, ; Dong et al., ; Gabay‐Laughnan, Chase, Ortega, & Zhao, ; Yue, Vick, Cai, & Hu, ) including sorghum (Jordan et al., , ; Klein et al., ; Praveen, Suneetha, Umakanth, Patil, & Madhusudhana, ).…”

Section: Introductionmentioning

confidence: 99%

Fine mapping of Rf2, a major locus controlling pollen fertility restoration in sorghum A₁ cytoplasm, encodes a PPR gene and its validation through expression analysis

et al. 2018

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Sorghum is one of the pioneering cereal crops where cytoplasmic male sterility (CMS) was successfully exploited for mass production of F1 hybrid seed. Mapping genes for fertility restoration (Rf) is an important aspect of understanding the molecular basis of fertility restoration in crop plants. In this study, we fine‐mapped a fertility restoration locus, Rf2 of sorghum reported earlier (Jordan, Mace, Henzell, Klein, & Klein, ), involving two F2 populations (296A × RS29 and 296A × DSV1) and newly developed SSR markers delimited Rf2 locus to 10.32‐kb region on chromosome 2. The Rf2 locus was tightly linked with two new SSRs, MS‐SB02‐3460 (0.14 cM) and MS‐SB02‐3466 (0.75 cM) on both sides, and hosted only one gene (Sobic.002G057050) of PPR gene family. Another new SSR marker developed in the study, MS‐SB02‐37912, forms the part of PPR gene and could act as a perfect marker in marker‐assisted breeding for fertility restoration involving Rf2 in sorghum breeding. The strong involvement of Sobic.002G057050 gene in fertility restoration was supported through RNA expression analysis.

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“…To date, several genes have been proven to act as CMS restorers, including Rf2 of maize (Cui et al, 1996); Rf-PPR591/592 of petunia (Bentolila et al, 2002); Rfs of rice (Akagi et al, 2004;Huang et al, 2012); Rfo, Rfob, and Rft of radish (Desloire et al, 2003;Wang et al, 2008;Yasumoto et al, 2009); Rf1 and Rf2 of sorghum (Klein et al, 2005;Jordan et al, 2010); and PePPR1 of pepper (Yeong et al, 2010). Fine-mapping of the Rf gene in soybean was also executed (Dong et al, 2012). These Rf genes all encode PPR-containing proteins except Rf2 in maize and Rf17 and Rf2 in rice (Fujii and Toriyama, 2009;Itabashi et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco

Ding

Chen

et al. 2014

Genet. Mol. Res.

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ABSTRACT. As a model plant, mechanisms of the cytoplasmic male sterility/restoration of fertility (CMS/Rf) system in tobacco are seldom studied. Using Rf gene sequences from other Solanaceae plants and the draft genome of Nicotiana benthamiana, degenerate primers were designed to amplify the cDNA pool of N. tomentosiformis. In total, six possible Rf sequences were identified, two of which contained basedeletion mutations. The other four were intact open reading frames, of which NtomPPR5 harbored a 3-pentatricopeptide repeat (PPR) motif deletion. Structure analysis revealed that they all encoded a PPRcontaining protein with putative mitochondrial targeting signals at their N-terminus, and they all belong to the P subfamily. Phylogenetic analysis showed that all of the Rf-coding PPRs clustered together, and recent duplication events might have occurred in tobacco after the divergence of the species. Quantitative reverse transcription polymerase Cloning and expression analysis of Rf genes in tobacco chain reaction analysis demonstrated that the NtomRfs were expressed in all tissues of N. tomentosiformis and (CMS) K326, although the expression levels varied with gene, organ, and developmental stage. Furthermore, the expression levels of Rf sequences in K326 were lower than those in CMS K326. The molecular basis of the CMS/Rf system in tobacco requires further investigation.

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Inheritance and fine mapping of a restorer-of-fertility (Rf) gene for the cytoplasmic male sterility in soybean

Cited by 21 publications

References 11 publications

Male sterility in soybean: Occurrence, molecular basis and utilization

Male sterility in soybean: Occurrence, molecular basis and utilization

Fine mapping of Rf2, a major locus controlling pollen fertility restoration in sorghum A₁ cytoplasm, encodes a PPR gene and its validation through expression analysis

Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco

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Inheritance and fine mapping of a restorer-of-fertility (Rf) gene for the cytoplasmic male sterility in soybean

Cited by 21 publications

References 11 publications

Male sterility in soybean: Occurrence, molecular basis and utilization

Male sterility in soybean: Occurrence, molecular basis and utilization

Fine mapping of Rf2, a major locus controlling pollen fertility restoration in sorghum A1 cytoplasm, encodes a PPR gene and its validation through expression analysis

Homology-based cloning and expression analysis of Rf genes encoding PPR-containing proteins in tobacco

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Fine mapping of Rf2, a major locus controlling pollen fertility restoration in sorghum A₁ cytoplasm, encodes a PPR gene and its validation through expression analysis