Inheritance and expression stability of exogenous genes in insect-resistant transgenic poplar

Ren, Yachao; Zhang, Jun; Liang, Haiyong; Wang, Jinmao; Yang, Minsheng

doi:10.1007/s11240-017-1247-y

Cited by 13 publications

(22 citation statements)

References 16 publications

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“…Hybridization was implemented using non-transgenic poplar 84K as the male parent trees, and transgenic poplar 741 lines showing different insect-resistant ability [high insect resistance (pB11, pB29), moderate insect resistance (pB1, pB17), and no insect resistance (pB6)] were used as the female parent trees. The insect resistance of the hybrid progeny plants was nearly identical to that of the parent plants (Ren et al, 2017). This study indicated that using transgenic poplars as parent plants for sexual hybridization could transfer exogenous genes to progeny, and lead to the cultivation of new insect-resistant species.…”

Section: Biosafety Assessmentmentioning

confidence: 80%

“…DBH growth in non-transgenic poplar 741 did not differ significantly from that of transgenic lines. This indicates that the exogenous Bt gene had no influence on the growth of Bt poplar (Ren et al, 2017). …”

Section: Biosafety Assessmentmentioning

confidence: 99%

“…Micromelalopha troglodyta was the most sensitive, followed by H. cunea , Clostera anachoreta , and L. dispar (Gao et al, 2004a). The average rate of lethal effects of the 741 transgenic poplar clones pB29 and pB11 on C. anachoreta 1st-instar larvae was 75–95%, H. cunea was lethal in >95% of cases in most years (Ren et al, 2017). The poplar 741 clone CC84 expressing the Cry3A gene modified the behavior of Coleopteran insects.…”

Section: Efficacy Of Gm Poplars In Pest Controlmentioning

confidence: 99%

“…Insect mortality fluctuated somewhat, but no regular decline was noted (Yang et al, 2005). Within the same year, different lines showed different degrees of resistances to the target pests, while differences within the same lines among years were also seen, which may be related to the climate, environment factors, and target pest status, among other factors (Ren et al, 2017). In the 8- and 10-year-old plantations, the diameter at breast height (DBH) of transgenic poplar 741 was also compared with that of non-transgenic poplar.…”

Section: Biosafety Assessmentmentioning

confidence: 99%

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The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China

et al. 2018

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Poplar is one of the main afforestation tree species in China, and the use of a single, or only a few, clones with low genetic diversity in poplar plantations has led to increasing problems with insect pests. The use of genetic engineering to cultivate insect-resistant poplar varieties has become a hot topic. Over the past 20 years, there have been remarkable achievements in this area. To date, nearly 22 insect-resistant poplar varieties have been created and approved for small-scale field testing, environmental release, or pilot-scale production. Here, we comprehensively review the development of insect-resistant genetically modified (GM) poplars in China. This review mostly addresses issues surrounding the regulation and commercialization of Bt poplar in China, the various insecticidal genes used, the effects of transgenic poplars on insects, toxic protein expression, multigene transformation, the stability of insect resistance, and biosafety. The efficacy of GM poplars for pest control differed among different transgenic poplar clones, larval instars, and insect species. The Bt protein analysis revealed that the expression level of Cry3A was significantly higher than that of Cry1Ac. Temporal and spatial studies of Bt protein showed that its expression varied with the developmental stage and tissue. The inheritance and expression of the exogenous gene were reviewed in transgenic hybrid poplar progeny lines and grafted sections. Biosafety issues, in terms of transgene stability and the effects on soil microorganisms, natural enemies of insects, and arthropod communities are also discussed.

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Section: Biosafety Assessmentmentioning

confidence: 80%

Section: Biosafety Assessmentmentioning

confidence: 99%

Section: Efficacy Of Gm Poplars In Pest Controlmentioning

confidence: 99%

Section: Biosafety Assessmentmentioning

confidence: 99%

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The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China

et al. 2018

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“…We determined whether the exogenous genes in transgenic poplars persisted during multiple asexual propagations and through years of field cultivation. Few studies have reported on the long-term efficacy of transformed pest-resistant poplars (Hu et al, 2001 ; Yang et al, 2005 ; Ren et al, 2017 ). This study presented a continuous and dynamic view of the insect resistance changes of different transgenic lines under field conditions.…”

Section: Discussionmentioning

confidence: 99%

The Relationship between Insect Resistance and Tree Age of Transgenic Triploid Populus tomentosa Plants

et al. 2018

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To explore the stability of insect resistance during the development of transgenic insectresistant trees, this study investigated how insect resistance changes as transgenic trees age. We selected 19 transgenic insect-resistant triploid Populus tomentosa lines as plant material. The presence of exogenous genes and Cry1Ac protein expression were verified using polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) analyses. The toxicity for Clostera anachoreta and Lymantria dispar was evaluated by feeding fresh leaves to first instar larvae after the trees were planted in the field for 2 years and after the sixth year. Results of PCR showed that the exogenous genes had a long-term presence in the poplar genome. ELISA analyses showed significant differences existed on the 6-year-old transgenic lines. The insect-feeding experiment demonstrated significant differences in the mortality rates of C. anachoreta and L. dispar among different transgenic lines. The average corrected mortality rates of C. anachoreta and L. dispar ranged from 5.6-98.7% to 35.4-7.2% respectively. The larval mortality rates differed significantly between the lines at different ages. Up to 52.6% of 1-year-old transgenic lines and 42.1% of 2-year-old transgenic lines caused C. anachoreta larval mortality rates to exceed 80%, whereas only 26.3% of the 6-year-old transgenic lines. The mortality rates of L. dispar exhibited the same trend: 89.5% of 1-year-old transgenic lines and 84.2% of 2-year-old transgenic lines caused L. dispar larval mortality rates to exceed 80%; this number decreased to 63.2% for the 6-year-old plants. The proportion of 6-year-old trees with over 80% larval mortality rates was clearly lower than that of the younger trees. The death distribution of C. anachoreta in different developmental stages also showed the larvae that fed on the leaves of 1-year-old trees were killed mostly during L 1 and L 2 stages, whereas the proportion of larvae that died in L 3 and L 4 stages was significantly increased when fed on leaves of 6-year-old trees. Results of correlation analysis showed there was a significant correlation between the larvae mortality rates of trees at different ages, as well as between Cry1Ac protein contents and larvae mortality rates of 6-year-old trees.

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Impacts of AlaAT3 transgenic poplar on rhizosphere soil chemical properties, enzyme activity, bacterial community, and metabolites under two nitrogen conditions

Yang,

Wang,

Niu

et al. 2024

GM Crops & Food

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Inheritance and expression stability of exogenous genes in insect-resistant transgenic poplar

Cited by 13 publications

References 16 publications

The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China

The Current Status and Development of Insect-Resistant Genetically Engineered Poplar in China

The Relationship between Insect Resistance and Tree Age of Transgenic Triploid Populus tomentosa Plants

Impacts of AlaAT3 transgenic poplar on rhizosphere soil chemical properties, enzyme activity, bacterial community, and metabolites under two nitrogen conditions

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