Inheritance and Allelic Relationships of Resistance Genes for Soybean mosaic virus in ‘Corsica’ and ‘Beeson’ Soybean

Abstract

Soybean mosaic virus (SMV) is an important viral disease of soybean [Glycine max (L.) Merr.] and causes a drastic reduction of seed yield and quality. Soybean mosaic virus isolates can be classified into one of seven strain groups, G1 through G7. Three loci with SMV resistance alleles Rsv1, Rsv3, and Rsv4 have been reported so far. Each locus contains multiple alleles: nine alleles at Rsv1, three alleles at Rsv3, and two alleles at Rsv4. Soybean cultivars Corsica (PI 559931) and Beeson (PI 548510) exhibit different patterns of response to SMV strains than differential cultivars with known genes. The objective of this study was to investigate the inheritance and allelism of resistance genes in Corsica and Beeson. To examine the inheritance of SMV resistance, Corsica and Beeson were crossed with the susceptible cultivar Essex, and the F2 and F3 progenies were inoculated with a G7 isolate. To identify the allelic relationship of genes in Corsica and Beeson with known SMV resistance loci, they were crossed with PI 96983 (Rsv1), ‘L29’ (Rsv3), and V94‐5152 (Rsv4), and their F2 plants and F2:3 lines were inoculated with G7. A serological tissue blotting immunoassay (TBIA) was used to confirm the presence or absence of the virus in the inoculated plants. Molecular markers were used to confirm the phenotypic data. The results showed a monogenic dominant resistance in Corsica, with the resistant gene located at the Rsv1 locus. Beeson also contains a single dominant gene for SMV resistance, but the gene is located at the Rsv4 locus. Both genes in Corsica and Beeson confer a different SMV reaction pattern than other reported alleles at the same loci. Therefore, we propose the new symbols of Rsv1‐c for the resistant gene in Corsica and Rsv4‐b for the resistant gene in Beeson. The results from the genetic study were confirmed by the molecular markers and TBIA. The information from this study can be used for parent selection by breeders in cross breeding and gene pyramiding.

Soybean mosaic virus: a successful potyvirus with a wide distribution but restricted natural host range

“…The Rsv1 (PI96983) locus has been found to be extremely complex, with at least 10 Rsv1 alleles recognized, namely and Rsv1-y (York) (Chen et al, 1991Ma et al, 1995Ma et al, , 2003Moon et al, 2009;Shakiba et al, 2013). Rsv1-y in York was recently renamed as a new gene, Rsv5, and mapped near Rsv1 (Chen et al, 1991;Klepadlo et al, 2017b).…”

“…4E). Alleles of Rsv4 with interactions different from G1 and G4 were identified in Beeson (Rsv4-b) and Shin2 Shakiba et al, 2013). The Rsv4-v allele in PI438307 (Klepadlo et al, 2016) gives reactions distinct from those of V94-5152 and PI88788 (Gunduz et al, 2004).…”

Wheat streak mosaic virus resistance in eight wheat germplasm lines

“…Similarly, soybean mosaic virus resistance gene Rsv1 has been found in at least ten resistant germplasm lines (Shakiba et al. ). However, we also found two resistant germplasm lines, PI243753 and PI321730, which might have different resistance genes than Wsm2 .…”

Identification of a New Allele at the Rsv3 Locus for Resistance to Soybean Mosaic Virus in PI 61944 Soybean Accession

“…An almost nondetectable level of the virus can be detected from necrotic plants. The Rsv4 locus has three alleles identified in V94-5152, PI 88788, and Beeson (Rsv4-b) that bring resistance to all seven strains (Buss et al, 1997;Ma et al, 2002;Gunduz et al, 2004;Shakiba et al, 2013). However, stem-tip necrosis is a hypersensitive reaction resulting from an incompatible reaction between the soybean and the SMV strain; thus, it should not be classified as a susceptible reaction (Buzzell and Tu, 1989;Chen et al, 1994).…”

“…The Rsv3 locus was identified at the LG B2 (chromosome 14) based on the data collected from two different F 2 populations, L29 (Rsv3) Lee68 (rsv3) and Tousan 140 (Rsv3) Lee68 (rsv3) (Jeong et al, 2002). The Rsv4 locus has three alleles identified in V94-5152, PI 88788, and Beeson (Rsv4-b) that bring resistance to all seven strains (Buss et al, 1997;Ma et al, 2002;Gunduz et al, 2004;Shakiba et al, 2013). The Rsv4 locus was reported in PI 486355 (Rsv1 + Rsv4) and confers resistance to all SMV strains, G1 to G7, and produces systemic necrosis in the heterozygous state in response to SMV infection.…”