Inherently hydrophilic mesoporous channel coupled with metal oxide for fishing endogenous salivary glycopeptides and phosphopeptides

Light, pH, and Temperature Triple-Responsive Magnetic Composites for Highly Efficient Phosphopeptide Enrichment

“…30 μL of skim milk or human saliva was dissolved in 1 mL of NH 4 HCO 3 buffer (50 mM, pH = 8.3). The mixture was treated at 100 °C for 10 min and cooled to room temperature and then treated with trypsin (1:40, w / w ) at 37 °C for 16 h. The obtained trypsin digest was stored at −20 °C for later use . 50 μL of healthy human serum was incubated in 450 μL of NH 4 HCO 3 buffer (50 mM, pH = 8.3) at room temperature for 1 h. The mixture was centrifuged at 3000 rpm for 5 min.…”

Self-assembly of hydrazide-linked porous organic polymers rich in titanium for efficient enrichment of glycopeptides and phosphopeptides from human serum

“…However, these materials sometimes suffer from difficulties in modification, redundancy in the synthesis process and weak adsorption capacities. 26 Porous organic polymers (POPs), a promising member of the organic polymer material family, are capable of capturing small targets in complex samples at interfaces and channels due to their advantages of excellent designability and high porosity. 27 However, addressing the hydrophobicity of POPs and the introduction of specific functional groups remains challenging.…”

Hydrophilic magnetic host–guest Ti–phenolic networks: a promising material for the highly sensitive enrichment of glycopeptides and phosphopeptides

“…1–3 As two of the important and extensive types of PTM, protein glycosylation and phosphorylation play a crucial role in various biological activities. 4–7 The mass spectrometry (MS) technology is a primary tool for the identification of glycopeptides and phosphopeptides. However, their abundance in complex biological samples is usually low and direct MS detection often suffers from the strong interference of a large number of unmodified peptides.…”