Inhaled Glucocorticoid Therapy of Childhood Asthma Is Associated With Reduced Peripheral Blood T Cell Activation and ‘Th2-Type’ Cytokine mRNA Expression

Gemou-Engesaeth, V.; Bush, Andrew; Kay, A. B.; Hamid, Qutayba; Corrigan, Christopher

doi:10.1542/peds.99.5.695

Cited by 57 publications

(44 citation statements)

References 26 publications

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“…15,16 In brief, inhaled glucocorticoid therapy was initiated or increased if asthma severity was not considered mild. Mild asthma was defined as absence of exercise and nocturnal symptoms, no significant loss of school time, requirement for no more than 3 doses of inhaled ␤2-agonists weekly, and lung function test (FEV1) above 85% of the predicted values, as described previously.…”

Section: Characteristics Of the Patients And Nonasthmatic Controlsmentioning

confidence: 99%

“…Mild asthma was defined as absence of exercise and nocturnal symptoms, no significant loss of school time, requirement for no more than 3 doses of inhaled ␤2-agonists weekly, and lung function test (FEV1) above 85% of the predicted values, as described previously. 16 Children were recruited from the Department of Pediatrics of Ullevål University Hospital.…”

Section: Characteristics Of the Patients And Nonasthmatic Controlsmentioning

confidence: 99%

“…Before lung function testing, 10 to 15 mL of peripheral venous blood was collected into heparin for isolation of mononuclear cells and full blood count, and serum isolated for total (Unicap, Pharmacia) and specific IgE measurements. Severity of symptoms was graded as done previously, 15,16 according to the method of Aas, 25 which was shown in a previous study 26 to correlate with eosinophil infiltration of the bronchial mucosa.…”

Section: Study Proceduresmentioning

confidence: 99%

“…Treatment was adjusted according to symptoms with the aim of rendering symptoms mild as recommended by international guidelines 22,23 in our previous study. 16 Follow-up for the purpose of the present study was conducted for a minimum of 4 months and a maximum of 6 months. Thereafter, follow-up was continued as clinically indicated.…”

Section: Study Proceduresmentioning

confidence: 99%

“…4 -9,13,14 We have used this observation in previous studies to demonstrate that in atopic child asthmatics as compared with controls matched for age and atopic status, elevated percentages of both CD4 and CD8 peripheral blood T cells expressed activation markers, 15 whereas elevated percentages of unfractionated peripheral blood mononuclear cells expressed mRNA encoding eosinophil-active cytokines including IL-5 and IL-4. 16 First, we wanted to extend these studies to examine cytokine production by both CD4 and CD8 T cells in child asthmatics. We hypothesized that both CD4 and CD8 T cells show elevated expression of mRNA encoding IL-5 and IL-4, along with activation markers, in child asthmatics as compared with controls.…”

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confidence: 99%

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Expression of Activation Markers and Cytokine mRNA by Peripheral Blood CD4 and CD8 T Cells in Atopic and Nonatopic Childhood Asthma: Effect of Inhaled Glucocorticoid Therapy

et al. 2002

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ABSTRACT. Hypothesis. Activated CD8 as well as CD4 T cells contribute to the production of asthma-relevant cytokines in both atopic and nonatopic childhood asthma.Objectives. To measure the percentages of peripheral blood CD4 and CD8 T cells expressing naïve/memory (CD45RA/CD45RO) and activation (HLA-DR, CD25) markers, as well as mRNA-encoding interleukin-4 (IL-4) and interleukin-5 (IL-5) in atopic and nonatopic childhood asthmatics and in nonasthmatic controls matched for age and atopic status; and to study the effects of inhaled glucocorticoid therapy of the asthmatics on these measurements.Methods. Peripheral blood mononuclear cells were isolated from 17 atopic and 8 nonatopic stable (not acutely ill) asthmatics aged 7 to 16 years with moderateto-severe disease and from 15 nonasthmatic controls matched for age and atopic status. Activation markers on CD4 and CD8 T cells were measured by flow cytometry, and expression of cytokine mRNA by in situ hybridization with CD4 and CD8 T cells were isolated using magnetic beads. Measurements were repeated in 18 of the asthmatics 4 to 6 months after initiation or escalation of inhaled glucocorticoid therapy for inadequately controlled asthma.Results. The percentages of CD4 T cells expressing CD45RO but not CD45RA were elevated in both asthma groups as compared with the relevant controls and were reduced in association with de novo or augmented inhaled glucocorticoid therapy. The percentages of CD8 T cells expressing both markers were not elevated in asthmatics as compared with controls. The percentages of both CD4 and CD8 T lymphocytes expressing HLA-DR and CD25 were elevated in the asthmatics as compared with controls, and significantly reduced in association with de novo or augmented inhaled glucocorticoid therapy. ABBREVIATIONS. IL-5, interleukin 5; IL-4, interleukin 4; IgE, immunoglobulin E; FEV1, forced expiratory volume in 1 second; PEF, peak expiratory flow; FITC, fluoroscein isothiocyanate; PE, phycoerythrin; PBS, phosphate-buffered saline; NK, natural killer.

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Section: Characteristics Of the Patients And Nonasthmatic Controlsmentioning

confidence: 99%

Section: Characteristics Of the Patients And Nonasthmatic Controlsmentioning

confidence: 99%

Section: Study Proceduresmentioning

confidence: 99%

Section: Study Proceduresmentioning

confidence: 99%

mentioning

confidence: 99%

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Expression of Activation Markers and Cytokine mRNA by Peripheral Blood CD4 and CD8 T Cells in Atopic and Nonatopic Childhood Asthma: Effect of Inhaled Glucocorticoid Therapy

et al. 2002

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Asthma history, occupational exposure to pesticides and the risk of non‐Hodgkin's lymphoma

Lee

Purdue

Stewart

et al. 2006

Intl Journal of Cancer

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We previously reported that, although asthma did not increase the risk of non-Hodgkin's lymphoma (NHL), the risk from pesticide exposures was higher among asthmatics than that among nonasthmatics. To further evaluate this finding, we analyzed data from a population-based case-control study of NHL conducted in Iowa, Detroit, Los Angeles and Seattle. Cases (n 5 668) diagnosed with NHL from 1998 to 2000 and controls (n 5 543) randomly selected from the same geographical areas as that of the cases were included in this analysis. Odds ratios (OR) for the risk of NHL from potential occupational exposure to pesticides tended to be higher among asthmatics (OR 5 1.7; 95% CI 0.3-9.1) when compared with that among nonasthmatics (OR 5 0.9; 95% CI 0.6-1.5). The risks of NHL associated with pesticide exposure were also higher among asthmatics who had history of hospitalization (OR 5 2.1; 95% CI 0.2-29.0) or daily medication for asthma (OR 5 infinite) than those among asthmatics who did not have such histories. Our results support the previous finding that the risk of NHL from pesticide exposure may be greater among asthmatics. ' 2006 Wiley-Liss, Inc.

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New therapies for asthma: Where next?

Robinson

2003

Pediatric Pulmonology

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Inhaled Glucocorticoid Therapy of Childhood Asthma Is Associated With Reduced Peripheral Blood T Cell Activation and ‘Th2-Type’ Cytokine mRNA Expression

Cited by 57 publications

References 26 publications

Expression of Activation Markers and Cytokine mRNA by Peripheral Blood CD4 and CD8 T Cells in Atopic and Nonatopic Childhood Asthma: Effect of Inhaled Glucocorticoid Therapy

Expression of Activation Markers and Cytokine mRNA by Peripheral Blood CD4 and CD8 T Cells in Atopic and Nonatopic Childhood Asthma: Effect of Inhaled Glucocorticoid Therapy

Asthma history, occupational exposure to pesticides and the risk of non‐Hodgkin's lymphoma

New therapies for asthma: Where next?

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