Inhalation of Hydrogen Attenuates Progression of Chronic Heart Failure via Suppression of Oxidative Stress and P53 Related to Apoptosis Pathway in Rats

Abstract

Background: Continuous damage from oxidative stress and apoptosis are the important mechanisms that facilitate chronic heart failure (CHF). Molecular hydrogen (H2) has potentiality in the aspects of anti-oxidation. The objectives of this study were to investigate the possible mechanism of H2 inhalation in delaying the progress of CHF.Methods and Results: A total of 60 Sprague-Dawley (SD) rats were randomly divided into four groups: Sham, Sham treated with H2, CHF and CHF treated with H2. Rats from CHF and CHF treated with H2 groups were injected isoprenaline subcutaneously to establish the rat CHF model. One month later, the rat with CHF was identified by the echocardiography. After inhalation of H2, cardiac function was improved vs. CHF (p < 0.05), whereas oxidative stress damage and apoptosis were significantly attenuated (p < 0.05). In this study, the mild oxidative stress was induced in primary cardiomyocytes of rats, and H2 treatments significantly reduced oxidative stress damage and apoptosis in cardiomyocytes (p < 0.05 or p < 0.01). Finally, as a pivotal transcription factor in reactive oxygen species (ROS)-apoptosis signaling pathway, the expression and phosphorylation of p53 were significantly reduced by H2 treatment in this rat model and H9c2 cells (p < 0.05 or p < 0.01).Conclusion: As a safe antioxidant, molecular hydrogen mitigates the progression of CHF via inhibiting apoptosis modulated by p53. Therefore, from the translational point of view and speculation, H2 is equipped with potential therapeutic application as a novel antioxidant in protecting CHF in the future.

Qiliqiangxin Attenuates Oxidative Stress-Induced Mitochondrion-Dependent Apoptosis in Cardiomyocytes <i>via</i> PI3K/AKT/GSK3β Signaling Pathway

“…22,23) Other studies have also indicated that luteolin and shengmai injection have antioxidant effects on the oxidative stress injury by up-regulating the HO-1 or CAT mRNA expression level in rat cardiomyocytes. 24,25) These are consistent with the results of the present study. In vivo, QLQX decreased serum LDH release and relative expression of ROS but increased serum SOD activity in rats with CHF post-AMI.…”

Hydrogen gas inhalation ameliorates cardiac remodelling and fibrosis by regulating NLRP3 inflammasome in myocardial infarction rats

“…The diverse biological functions of the hydrogen are still being uncovered. The ability of hydrogen to inhibit apoptosis and regulate metabolism has also been effectively proved 32 . Notably, oxidative stress is one of the key ways in which NLRP3 is activated 33 .…”

Effects of long-term hydrogen intervention on the physiological function of rats

“…Previous studies showed that H 2 administration protects against myocardial infarction 20 – 23 , heart failure 24 , 25 , toxin-induced myocardial injury 26 , 27 and improves cardiac function induced by hypertension 28 , 29 and transplantation injury 30 , 31 . The most striking changes were observed in serum myocardial enzymes spectrum in our study.…”