Ingested quercetin but not rutin increases accumulation of hepatic β‐carotene in BALB/c mice

Abstract

Beta-carotene is a carotenoid with a range of reported health benefits besides vitamin A activity. If the enzymatic conversion of beta-carotene to retinal is suppressed in the digestive tract, residual beta-carotene that reaches the tissues increases. We evaluated the function of quercetin and rutin (quercetin-3-rutinoside) to increase the accumulation of beta-carotene in vitro and in vivo in BALB/c mice. When the conversion of beta-carotene by a preparation of the murine small intestine was measured in vitro, the addition of quercetin or rutin considerably inhibited the conversion. When the levels of hepatic beta-carotene and retinoids were measured among three groups of mice fed a diet supplemented with beta-carotene plus quercetin or rutin or beta-carotene alone (four to six mice per group), quercetin increased the level of beta-carotene and decreased the level of retinol, whereas rutin did not. These results demonstrate that quercetin can suppress the conversion of beta-carotene which develops in the cytosol of small intestinal epithelial cells, and that rutin whose rutinose-moiety prevents being absorbed in the small intestine cannot suppress the conversion in vivo. This study offers a novel insight into the interaction between flavonoids and carotenoids with respect to the health benefits from the latter.

Increased Exposure of Vitamin A by Chrysanthemum morifolium Ramat Extract in Rat Was Not via Induction of CYP1A1, CYP1A2, and CYP2B1

“…A study revealed that luteolin, quercetin, and rutin, active compounds in CM, may noncompetitively inhibit the activity of β‐carotene‐15, 15′‐monooxygenase extracted from intestine (Nagao and others 2000). Feeding with diet containing quercetin or rutin did not alter level of hepatic retinol in BALB/mice, but decreased level of hepatic retinoic acid (Bando and others 2010). This suggested that more β‐carotene absorbed might be converted to retinol.…”

Interaction between Flavonoids and Carotenoids on Ameliorating Oxidative Stress and Cellular Uptake in Different Cells

“…The antioxidant interactions and the bioavailability interactions among phytochemicals were often measured independently, however, rare study investigated their correlation [ 23 ]. Several studies have revealed the role of flavonoids in the cell uptake of carotenoids without further determining their influence on the biological effects of carotenoids [ 11 , 24 , 25 , 26 ]. The absorption, distribution, and metabolism of carotenoids varied in different tissues.…”

“…Therefore, the detected lycopene and lutein content is lower in L-02 cells than in Caco-2 cells. Flavonoids were considered to interfere with the absorption of carotenoids in several studies [ 11 , 12 , 24 , 25 , 26 , 29 ]. In the case of naringenin and lutein, competition between them for cellular uptake led to the reduced lutein uptake in vivo [ 11 ].…”

Chemical study of Dodonaea viscosa planting in Iraq

“…The decreased hormone level can be explained by its inhibitory effect produced on Thyroid peroxidase enyzme (TPO) ( Metodiewa et al, 1997) rutin is also an antioxidant compared to quercetin, acacetin, morin, hispidulin, hesperidin, and naringin, it was found to be the strongest ( Bando et al, 2010) Siddiqui (1998) survey makes reference to eighteen flavonoids including glycosides of quercetin (rutin) and isorhamnetin. Mata et al (1991) and colleagues segregated sakuranetin from Mexican D. viscosa in 1991. it is a yellow crystalline solid that has been used to dye wool (Fig.4) (Mabry, 1970).…”