Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation

Abstract

Abstract:Objective: To study the effect and implication of nonmyeloablative donor specific bone marrow (DSBM) infusion on the immunoreaction of liver allotransplantation. Methods: Orthotopic liver transplantation model was used in this study. Groups were set as follows: Group I, syngeneic control (Wistar-to-Wistar); Group II, acute rejection (SD-to-Wistar); Group III, acute rejection treated with cyclosporine A (CsA) by intramuscular injection (SD-to-Wistar+CsA); Group IV, bone marrow infusion at 7 d pretransplantation followed by short-term CsA treatment (SD-to-Wistar+DSBM); Another group of short-term CsA treatment preoperatively without bone marrow infusion was also set as control. General characteristics and survival time were observed. Histological grades of rejection were determined by pathological examination. IL-2 and IFN-γ level in peripheral blood and donor liver were detected respectively by Enzyme-Linked Immuno-Sorbent Assay (ELISA) and Western blot. Chimerism of donor cells was measured by PCR for a male-specific marker (Y-chromosome-specific sequence, Sry). Results: No signs of rejection were found in Group I. Acute rejection occurred in both Group II and the short-term CsA treated group. All the recipients died at (9~15) d posttransplantation with a median survival time of (10.7±0.5) d and (11.2±2.4) d, respectively. Only mild rejection could be seen in Group III. In Group IV, 4 out of 6 recipients had long-term survival (>100 d), the histological grade of rejection was significantly lower than that of Group II, so did the expression level of IL-2 and IFN-γ in both peripheral blood and grafted liver. Y-chromosome-specific sequence (Sry) of male SD rats could be detected in the bone marrow, spleen and thymus of female recipients at 15 d after bone marrow infusion. Conclusion: Mild preconditioning nonmyeloablative donor specific bone marrow infusion can enhance chimerism formation in recipients, alleviate the rejection of liver allotransplantation and prolong survival of liver allotransplantation.

Comparative Study on the Differentiation of Mesenchymal Stem Cells between Fetal and Postnatal Rat Spinal Cord Niche

“…In the present study, we choose Wistar rats to perform the MSC transplantation. There is minimal rejection after organ transplantation from Wistar to Wistar, and it serves as a syngeneic control in many studies (56,68). Moreover, in the present study, the Wistar rat was transplanted with the same species of MSCs that possess immunosuppressive properties and may not induce an immune response.…”

Liberty and Order: Legal Interests in China's Domestic Crime Legislation and Amendment

“…Guizhou, and Sichuan, accounting for two-thirds of the married young women in the village. 2 Therefore, buying abducted women is a common phenomenon in the area, and mainstream society does not consider it to be a crime. This is because it is a custom that has been passed down for generations.…”