Infusion of Cytotoxic T Cells for the Prevention and Treatment of Epstein-Barr Virus–Induced Lymphoma in Allogeneic Transplant Recipients

Abstract

Epstein-Barr virus (EBV) causes potentially lethal immunoblastic lymphoma in up to 25% of children receiving bone marrow transplants from unrelated or HLA-mismatched donors. Because this complication appears to stem from a deficiency of EBV-specific cytotoxic T cells, we assessed the safety and efficacy of donor-derived polyclonal (CD4+ and CD8+) T-cell lines as immunoprophylaxis and treatment for EBV-related lymphoma. Thirty-nine patients considered to be at high risk for EBV-induced lymphoma each received 2 to 4 intravenous infusions of donor-derived EBV-specific T lymphocytes, after they had received T-cell–depleted bone marrow from HLA-matched unrelated donors (n = 33) or mismatched family members (n = 6). The immunologic effects of this therapy were monitored during and after the infusions. Infused cells were identified by detection of the neo marker gene. EBV-specific T cells bearing theneo marker were identified in all but 1 of the patients. Serial analysis of DNA detected the marker gene for as long as 18 weeks in unmanipulated peripheral blood mononuclear cells and for as long as 38 months in regenerated lines of EBV-specific cytotoxic T cells. Six patients (15.5%) had greatly increased amounts of EBV-DNA on study entry (>2,000 genome copies/106 mononuclear cells), indicating uncontrolled EBV replication, a complication that has had a high correlation with subsequent development of overt lymphoma. All of these patients showed 2 to 4 log decreases in viral DNA levels within 2 to 3 weeks after infusion and none developed lymphoma, confirming the antiviral activity of the donor-derived cells. There were no toxic effects that could be attributed to prophylactic T-cell therapy. Two additional patients who did not receive prophylaxis and developed overt immunoblastic lymphoma responded fully to T-cell infusion. Polyclonal donor-derived T-cell lines specific for EBV proteins can thus be used safely to prevent EBV-related immunoblastic lymphoma after allogeneic marrow transplantation and may also be effective in the treatment of established disease. © 1998 by The American Society of Hematology.

Post cardiac transplantation lymphoproliferative disorder presenting as t(8;14) Burkitt leukaemia/lymphoma treated with low intensity chemotherapy and rituximab

“…It has been suggested that for intermediate and advanced stages of PTLD in adults and children, the combination of chemotherapy and Rituximab may be preferable to ensure durable remission [22][23][24]. In bone marrow transplant patients who develop PTLD, infusion of donor EBV specific CTL has been reported to induce remission [25]. A similar strategy for solid organ transplant recipients is difficult to adopt owing to the non-availability of a suitable source of cells, however, partially HLA-matched, banked CTL have been used to successfully treat PTLD in children and adults with a variety of solid organ transplants [26].…”

Subacute immune response to primary EBV infection leading to post-transplant lymphoproliferative disease in a renal transplant patient

“…Other therapeutic options for the treatment of PTLD include: interferon-alpha, chemotherapy, antivirals, and anti-B-cell monoclonal antibodies (e.g rituximab; Milpied et al, 2000). EBV-specific allogeneic cytotoxic lymphocytes, grown in vitro and given in the peri-transplant period, have been tried for prevention and treatment (Rooney et al, 1995(Rooney et al, , 1998Haque et al, 2002). However, the primary therapy of EBV + PTLD is a controlled, gradual reduction of immunosuppression, wherever possible.…”

Medicinal Chemistry of Fluorinated Cyclic and Acyclic Nucleoside Phosphonates

“…163 From those analogues, compound 147 exhibited interesting activity on both human cytomegalovirus (HMCV, EC 50 = 5.6 μg/mL) 164,165 and EBV (EC 50 = 1.6 μg/mL). [166][167][168][169] The α-monofluorinated vinylphosphonate analogues 153 and 154 (Fig. 42) with the nucleobase attached to the γ -carbon of the phosphonate moiety were prepared as novel analogues of unsaturated phosphononucleosides, 170 but 153 and 154 diethyl esters have not been deprotected to determine their biological activity.…”