Infusible platelet membrane microvesicles: a potential transfusion substitute for platelets

Chao, Francis C.; Kim, B. K.; Houranieh, A. M.; Liang, Fulong; Konrad, Michael; Swisher, Scott N.; Tullis, James L.

doi:10.1046/j.1537-2995.1996.36696269513.x

Cited by 70 publications

(52 citation statements)

References 33 publications

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“…Infusible platelet membranes (IPM) prepared from new or outdated human platelets have been developed as an alternative to standard PCs. These microparticles have additional advantages like long shelf life, increased viral safety and decreased adverse reactions due to the removal of plasma [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

Yari

Ahmadzadeh

Azadpour

et al. 2011

Indian J Hematol Blood Transfus

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The demand for standard platelet concentrates (PCs) has continued to increase in the recent years. Infusible platelet membranes (IPM) prepared from new or outdated human platelets have been developed as an alternative to standard PCs, with the additional advantage of long shelf life and increased viral safety. Reduction of HLA antigens on the IPM has been assigned as one of the probable advantages of this product. In re-examining this issue, we studied the existence of HLA class I on the surface of IPM microparticles. In comparison we also surveyed HLA expression on the surface of the naturally occurred platelet-derived microparticles (nPMPs) during 7 days storage. Intended for producing IPM, PCs obtained from Iranian blood transfusion organization were lysed; virally inactivated with wet heat in the presence of a heat stabilizer and then sonicated. IPMs were separated using centrifugation and liquid-stored in 4°C. The expression of HLA class I antigens was surveyed using flow cytometry technique. HLA molecules were present on the microparticles. Shedding of HLA antigens was demonstrated from the surface of the both liquid-stored IPM and nPMPs during storage. Storage of IPM in 4°C was accompanied with significant reduction of HLA molecules. It seemed that achievement of HLA-free IPM could be impossible unless chloroquine treated platelets were used to prepare these microvesicles.

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Section: Introductionmentioning

confidence: 99%

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

Yari

Ahmadzadeh

Azadpour

et al. 2011

Indian J Hematol Blood Transfus

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“…In this research, the retention of gpIIb/IIIa and gpIba molecules was demonstrated on the surface of plateletderived IPM microparticles using flow cytometry that is the usual method for the study of microparticles [18]. The obtained results had conflict with the previous report related to the absence of gpIIb/IIIa molecules on the surface of produced IPM [10]. The described difference may be related to the age dissimilarity in the platelets that were used for the production of IPM.…”

Section: Discussionmentioning

confidence: 67%

“…The described difference may be related to the age dissimilarity in the platelets that were used for the production of IPM. We used 3-5 daysold PCs whereas Chao et al [10] had employed 10-19 days-old PCs. Alternatively, the main cause of the difference may be related to the preparation method as its effect was elucidated in our study using different concentrations of the heat stabilizer but the use of heat protection method were not explained in the work of Chao.…”

Section: Discussionmentioning

confidence: 99%

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Glycoproteins of GpIbα and GpIIbIIIa on the Synthetic or Naturally Occurred Platelet-Derived Microparticles

Azadpour

Yari

Ahmadzadeh

2012

Indian J Hematol Blood Transfus

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“…Метод получения и лиофилизации таких везикул для переливания был впервые успешно разработан одной из биотехнологических компаний в США в конце 1990-х гг. [50]. Они действительно сохраняли частичную функциональность; первые испытания на животных и первые фазы клинических испытаний показали их способность сокращать время кротовотечения (рис.…”

Section: Fig 6 Formation Of Microvesicles During Activation Of Platunclassified

Physiology and pathology of extracellular vesicules

Пантелеев¹,

Абаева²,

Нечипуренко³

et al. 2017

Onkogematologiâ

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ВведениеВнеклеточные везикулы (ВВ) представляют собой бислойные структуры из мембранных липидов, кото-рые присутствуют во всех жидкостях нашего организ-ма: интерстициальной, спинномозговой, амниотиче-ской и других, а также в слюне, сперме, плазме крови, лимфе. Везикулы крови были открыты самими первы-ми и остаются наиболее изученными до сих пор. Их размер варьирует в широких пределах -от десятков нанометров до микрона и более, форма чаще всего сферическая (особенно для небольших). ВВ образуют-ся при разнообразных процессах активации, жизнеде-ятельности и смерти практически из любых клеток. В зависимости от происхождения и устройства, среди ВВ выделяют экзосомы, микровезикулы, апоптотиче-ские тела и другие подтипы (впрочем, эта классифика-ция пока плохо проработана и не устоялась). В отличие от клеток, даже самых примитивных, ВВ «мертвы»: у них нет мембранной и ионной асимметрии, зависи-мых от аденозинтрифосфата (АТФ) процессов и про-чих признаков даже самых примитивных клеток. Од-нако они несут на себе и внутри себя разнообразные

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Infusible platelet membrane microvesicles: a potential transfusion substitute for platelets

Abstract: IPM may have clinical potential as a substitute for platelets in the treatment of bleeding due to thrombocytopenia.

Cited by 70 publications

References 33 publications

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

HLA Antigens Shed from the Surface of Synthetic or Naturally Occurred Platelet-Derived Microparticles During Storage of Platelet Concentrate

Glycoproteins of GpIbα and GpIIbIIIa on the Synthetic or Naturally Occurred Platelet-Derived Microparticles

Physiology and pathology of extracellular vesicules

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