Infused neutrophil dose and haematopoietic recovery in patients undergoing autologous transplantation

Bai, Lijun; Xia, Wei; Wong, Kelly; Reid, Cassandra; Ward, Christopher; Greenwood, Matthew

doi:10.1038/bmt.2014.14

Cited by 2 publications

(1 citation statement)

References 8 publications

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“…The RBC count, PLT count, and neutrophil percentage of the HPC‐A are important parameters to define the quality of the collected product: the lower the contamination of the HPC‐A with these cells, the higher the collection purity. It has been demonstrated that increasing neutrophil contamination of HPC‐A is associated with slower neutrophil engraftment after autologous SCT . Furthermore, when a major RBC antigen incompatibility exists between the donor and recipient of an allogeneic SCT (i.e., the recipient carries or can produce antibodies directed against antigens of the donor RBC in the HPC‐A), the collection, processing, and infusion regimen of the HPC‐A will be guided by the RBC count or HCT of the HPC‐A to avoid transfusion reactions.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of two automated cell counters for the analysis of hematopoietic progenitor cell apheresis products

Gils

Cauwelier

Devos

et al. 2017

Int J Lab Hematology

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Section: Discussionmentioning

confidence: 99%

Evaluation of two automated cell counters for the analysis of hematopoietic progenitor cell apheresis products

Gils

Cauwelier

Devos

et al. 2017

Int J Lab Hematology

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Viability of mononuclear cells in leukocyte concentrates at the stages of their preparation, freezing, and thawing

et al. 2023

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Aim. To evaluate the viability of mononuclear cells (MNCs) in leukocyte concentrates (LCs) at the stages of their preparation, freezing, and thawing.Materials and methods. The study material included 44 LCs from donors of allogeneic hematopoietic stem cells (HSCs) and 189 autologous LCs from patients with oncohematological disorders. LCs were obtained from donors and patients by leukocytapheresis after mobilization of HSCs. LCs from patients were frozen with dimethyl sulfoxide (DMSO) used as a cryoprotectant at a final concentration of 5% and stored in liquid nitrogen. LCs were thawed before transplantation. A total of 161 LCs were immediately transfused to the recipient after thawing, and 28 LCs were washed from DMSO before transfusion. Flow cytofluorometry was used to determine the percentage of MNC populations that excluded 7-aminoactinomycin D (7-AAD).Results. The viability of peripheral blood MNCs in donors and patients was close to 100%. It was found that leukocytapheresis and cryopreservation with DMSO did not affect the viability of MNCs. The freezing of LCs with DMSO, storage in liquid nitrogen, and thawing resulted in a significant decrease in the content of viable MNCs (p = 0.0025), while no effect of LC storage duration on the viability of MNCs was revealed. Following DMSO removal from LCs, significantly more HSCs remained in a viable state than without washing (94.4 [94.5; 95.2] % vs. 86.7 [67.6; 92.9] %, (p = 0.0051); for other MNC populations, except monocytes, the differences in the viability index were also statistically significant.Conclusion. The viability of MNCs in LCs is recommended to be used as an independent characteristic of the transplant quality. In obtaining LCs and mixing them with the cryoprotectant DMSO, the viability of MNCs does not decrease, while in thawed LCs, it decreases significantly. Thawing of LCs with removal of DMSO allows to achieve the best viability of HSCs and most MNC populations.

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Infused neutrophil dose and haematopoietic recovery in patients undergoing autologous transplantation

Cited by 2 publications

References 8 publications

Evaluation of two automated cell counters for the analysis of hematopoietic progenitor cell apheresis products

Evaluation of two automated cell counters for the analysis of hematopoietic progenitor cell apheresis products

Viability of mononuclear cells in leukocyte concentrates at the stages of their preparation, freezing, and thawing

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