Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

Sardelić, Sanda; Bedenić, Branka; Colinon-Dupuich, Céline; Orhanović, Stjepan; Bošnjak, Zrinka; Plečko, Vanda; Cournoyer, Benoît; Rossolini, Gian María

doi:10.1128/aac.05212-11

Cited by 24 publications

(21 citation statements)

References 31 publications

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“…A higher prevalence of MBL production was observed among IMP-NS P. aeruginosa isolates (33%) than in other studies from Greece and other European countries (14,15). All MBL-positive isolates in the present study carried the bla VIM gene and were spread in all hospital wards, especially among non-ICU patients (Table 1).…”

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confidence: 43%

Spread of Multidrug-Resistant Pseudomonas aeruginosa Clones in a University Hospital

et al. 2013

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An outbreak of multidrug-resistant Pseudomonas aeruginosa (MDRPA) infections in a university hospital is described. Phenotypic and genotypic analysis of 240 isolates revealed that 152 patients, mainly in the intensive care unit (ICU), were colonized or infected with MDRPA, the majority with O11. All metallo-␤-lactamase (MBL)-positive isolates carried the bla VIM-2 or bla VIM-1 gene. One or more type III secretion system toxin genes were detected in most isolates. Five dominant pulsed-field gel electrophoresis (PFGE) types were characterized, associated with ST235, ST111, ST253, ST309, and ST639.

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confidence: 43%

Spread of Multidrug-Resistant Pseudomonas aeruginosa Clones in a University Hospital

et al. 2013

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“…Enormous spread of MBL-producing ST235 has been observed in Russia over the 2000s (14). ST111 and CC111 have often been reported as well (3,11,15,20,22,26,27). ST111 has been prevalent among MDR P. aeruginosa isolates in France (11%) (3) and MBL producers in Greece; recently, MBL-positive ST111 has spread in the Netherlands (27).…”

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confidence: 99%

Sequence Types 235, 111, and 132 Predominate among Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates in Croatia

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et al. 2014

Antimicrob Agents Chemother

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“…This lineage belongs to the O12 serotype, which has been associated with multidrug resistance and expansion in hospitals for decades (1,11). It has been described in many European and Mediterranean countries, including France, Italy, Greece, the Netherlands, Spain and the Canary islands, Hungary, Scandinavia, Croatia, Austria, and Germany (6,(12)(13)(14)(15)(16)(17)(18)(19)(20)(21) as well as in the United Kingdom (22,23). It has been frequently associated with VIM-2 or less commonly with VIM-4 or IMP-type MBLs but sometimes only with noncarbapenemase beta-lactamases, such as VEB-1 and OXA extendedspectrum beta-lactamases (24,25).…”

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High-Resolution Analysis by Whole-Genome Sequencing of an International Lineage (Sequence Type 111) of Pseudomonas aeruginosa Associated with Metallo-Carbapenemases in the United Kingdom

et al. 2015

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(1); one isolate had VIM-2 and IMP-18, and 7 carried no metallo-beta-lactamase (MBL) gene. Single nucleotide polymorphism analysis divided the isolates into distinct clusters; the NDM-1 isolate was an outlier, and the IMP isolates and 6/7 MBL-negative isolates clustered separately from the main set of 73 VIM-2 isolates. Within the VIM-2 set, there were at least 3 distinct clusters, including a tightly clustered set of isolates from 3 hospital laboratories consistent with an outbreak from a single introduction that was quickly brought under control and a much broader set dominated by isolates from a long-running outbreak in a London hospital likely seeded from an environmental source, requiring different control measures; isolates from 7 other hospital laboratories in London and southeast England were also included. Bayesian evolutionary analysis indicated that all the isolates shared a common ancestor dating back ϳ50 years (1960s), with the main VIM-2 set separating approximately 20 to 30 years ago. Accessory gene profiling revealed blocks of genes associated with particular clusters, with some having high similarity (>95%) to bacteriophage genes. WGS of widely found international lineages such as ST-111 provides the necessary resolution to inform epidemiological investigations and intervention policies. Concern over the increasing prevalence in hospitals of multiresistant bacteria, especially those that are resistant to carbapenem antibiotics, has prompted the use of typing methods that easily allow interlaboratory comparison of isolates. As a result, it has become clear that (i) high-risk clones of various Gram-negative bacteria, including Pseudomonas aeruginosa, can be found in many disparate locations, often with no obvious epidemiological links between the affected patients, and (ii) many of these clones are adept at acquiring various antibiotic resistance determinants (1, 2). These clones are commonly referred to as international lineages since they have been described in many countries across the globe. Epidemiological investigations of apparent outbreaks and interhospital spread of such lineages are confounded by the fact that they are so widely found; microbiological evidence of the same type must be considered alongside information about patient location and movement, for example, and patient location and movement information should be sufficiently robust to allow firm conclusions to be drawn. Greater resolution than that provided by conventional molecular typing methods, such as multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and variable-number tandem repeat (VNTR) analysis, is essential if we are to infer or discount potential transmission pathways with confidence. Understanding these pathways will be critical for precise public health interventions to contain these highrisk clones effectively. Whole-genome sequencing (WGS) can provide this resolution and has been used to investigate outbreaks of Klebsiella pneumoniae ST-258 producing K. pneumoniae carbapenemase (KPC) enz...

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Infrequent Finding of Metallo-β-Lactamase VIM-2 in Carbapenem-Resistant Pseudomonas aeruginosa Strains from Croatia

Cited by 24 publications

References 31 publications

Spread of Multidrug-Resistant Pseudomonas aeruginosa Clones in a University Hospital

Spread of Multidrug-Resistant Pseudomonas aeruginosa Clones in a University Hospital

Sequence Types 235, 111, and 132 Predominate among Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates in Croatia

High-Resolution Analysis by Whole-Genome Sequencing of an International Lineage (Sequence Type 111) of Pseudomonas aeruginosa Associated with Metallo-Carbapenemases in the United Kingdom

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