Infrasound induces coronary perivascular fibrosis in rats

Lousinha, Ana; Oliveira, Maria João; Borrecho, Gonçalo; Brito, José; Oliveira, Pedro; Carvalho, António Oliveira de; Freitas, Diamantino; Águas, Artur P.; Antunes, Eduardo

doi:10.1016/j.carpath.2018.10.004

Cited by 15 publications

(12 citation statements)

References 42 publications

(64 reference statements)

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“…The present study evaluated two specific features of myocardial remodeling in rat heart, interstitial fibrosis and Cx43 modifications, as a consequence of exposure to IFS (120 dB, < 20 Hz) and validated our initial hypothesis that exposure to different periods of high-intensity IFS can lead to structural atrial remodeling. These results are consistent with those of past investigations from our group, also in Wistar rats (Oliveira et al, 2005;Oliveira et al, 2012;Oliveira et al, 2013;Antunes et al, 2013a;Lousinha et al, 2015;Antunes et al, 2013b;Antunes et al, 2013c;Antunes et al, 2013d;Lousinha et al, 2018), confirming the abnormal proliferation of connective tissue as the main morphological change induced by LFN exposure. In our study, Cx43 also appears to undergo age-dependent loss, as lower concentrations were found among ≥22 weeks-old controls compared to younger rats (16 weeks-old), a result that comes in agreement with previously reported data from Watanabe et al (Watanabe et al, 2004).…”

Section: Discussionsupporting

confidence: 94%

“…In the cardiovascular system of rodents, we reported the development of perivascular fibrosis around the coronary arteries of rats exposed to industrial noise (Antunes et al, 2013a;Lousinha et al, 2015) and documented a significant fibrotic development in ventricular myocardium among rats submitted to LFN (Antunes et al, 2013b;Antunes et al, 2013c), together with a possible ventricular gap junction remodeling (Antunes et al, 2013d). We also found that IFS exposure induces coronary perivascular fibrosis (Lousinha et al, 2018).…”

Section: Introductionsupporting

confidence: 52%

“…The electroacoustic experiment was described before (Lousinha et al, 2018). In summary, a pseudo-random waveform in the 2 to 20 Hz decade band was designed with Matlab based on a bandpass-filtered 30s maximum length sequence segment and the resulting acoustic pressure waveform involved an average sound pressure level of 120 dB with a tolerance of ± 3 dB.…”

Section: Electroacoustic Experimentsmentioning

confidence: 99%

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Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound

Lousinha

Pereira

Borrecho

et al. 2020

Experimental and Molecular Pathology

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Background: Noise is an important environmental risk factor. Industrial environments are rich in high-intensity infrasound (hi-IFS), which we have found to induce myocardial and coronary perivascular fibrosis in rats. The effects of exposure to IFS on the ventricles have been studied, but not on the atria. We hypothesized that rats exposed to hi-IFS develop atrial remodeling involving fibrosis and connexin 43, which we sought to evaluate. Material and methods: Seventy-two Wistar rats, half exposed to hi-IFS (120 dB, < 20 Hz) during a maximum period of 12 weeks and half age-matched controls, were studied. Atrial fibrosis was analyzed by Chromotropeaniline blue staining. The immunohistochemical evaluation of Cx43 was performed using the polyclonal antibody connexin-43 m diluted 1:1000 at 4°C overnight. Digitized images were obtained with an optical microscope using 400× magnifications. The measurements were performed using image J software. A two-way ANOVA model was used to compare the groups. Results: The mean values of the ratio "atrial fibrosis / cardiomyocytes" increased to a maximum of 0.1095 ± 0,04 and 0.5408 ± 0,01, and of the ratio "CX43 / cardiomyocytes" decreased to 0.0834 ± 0,03 and 0.0966 ± 0,03, respectively in IFS-exposed rats and controls. IFS-exposed rats exhibited a significantly higher ratio of fibrosis (p < .001) and lower ratio of Cx43 (p = .009). Conclusion: High-intensity infrasound exposure leads to an increase in atrial interstitial fibrosis and a decrease in connexin 43 in rat hearts. This finding reinforces the need for further experimental and clinical studies concerning the effects of exposure to infrasound.

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Section: Discussionsupporting

confidence: 94%

Section: Introductionsupporting

confidence: 52%

Section: Electroacoustic Experimentsmentioning

confidence: 99%

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Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound

Lousinha

Pereira

Borrecho

et al. 2020

Experimental and Molecular Pathology

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“…No changes were observed in the lumen-to-wall ratio. With dexamethasone treatment and exposure, no differences were observed in the wall-to-perivascular-space ratio, as compared to controls, suggesting an underlying inflammatory mechanism [46].…”

Section: Heart Cells and Tissuesmentioning

confidence: 79%

Acoustics and Biological Structures

Alves-Pereira¹,

Rapley²,

Bakker³

et al. 2019

Acoustics of Materials

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Within the context of noise-induced health effects, the impact of airborne acoustical phenomena on biological tissues, particularly within the lower frequency ranges, is very poorly understood. Although the human body is a viscoelasticcomposite material, it is generally modeled as Hooke elastic. This implies that acoustical coupling is considered to be nonexistent at acoustical frequencies outside of the human auditory threshold. Researching the acoustical properties of mammalian tissue raises many problems. When tissue samples are investigated as to their pure mechanical properties, stimuli are not usually in the form of airborne pressure waves. Moreover, since the response of biological tissue is dependent on frequency, amplitude, and time profile, precision laboratory equipment and relevant physiological endpoints are mandatory requirements that are oftentimes difficult to achieve. Drawing upon the viscoelastic nature of biological tissue and the tensegrity model of cellular architecture, this chapter will visit what is known to date on the biological response to a variety of different acoustic stimuli at very low frequencies.

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“…There is evidence from high-intensity infrasound exposure studies in laboratory animals that chronic exposure results in proliferation of the connective tissue matrix and collagen fibers in animals; this fibrotic response has been documented in several organs, such as the heart, lung and glands of rats chronically exposed to industrial-type noise [ 27 , 28 , 29 , 30 ]. Oliveira et al.…”

Section: Discussionmentioning

confidence: 99%

Effects of high-intensity infrasound on liver lipid content of rats

Pereira

Santos

et al. 2020

Heliyon

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Previous experimental studies show that exposure to noise with high and audible frequencies causes multiple metabolic alterations, such as increased liver glycogen and triglycerides. However, the effect of exposure to sound with lower frequencies, such as high-intensity infrasound (frequency <20 Hz and sound pressure level >90 dB), on the liver lipid content is still unclear. As such, we aimed to study the effect of exposure to high-intensity infrasound of both normal and glucose intolerant rats on the liver lipid content. For this study, 79 wild-type male Wistar rats were randomly divided into two groups: G1, no treatment, and G2, induced glucose intolerance. Each of these two groups was randomly divided in two subgroups: s (animals kept in silence) and i (animals continuously exposed to high-intensity infrasound noise). At three noise-exposure time-points (1, 6 and 12 weeks) the rats were sacrificed, the liver was excised and hepatic lipids extracted. Data analysis was performed using a two-way ANOVA (p = 0.05). No significant effects due to interactions between the several factors exist on the liver lipid content (p=0.077). Moreover, no significant effects due to infrasound exposure (p=0.407) or glucose tolerance status (p=0.938) were observed. Our study shows that continuous exposure to high-intensity infrasound has no influence on the lipid content of the liver of both normal and glucose intolerant animals. This finding reinforces the need for further experimental studies on the physiological effects of infrasound due to its possible hazardous effects on human health.

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Infrasound induces coronary perivascular fibrosis in rats

Cited by 15 publications

References 42 publications

Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound

Atrial fibrosis and decreased connexin 43 in rat hearts after exposure to high-intensity infrasound

Acoustics and Biological Structures

Effects of high-intensity infrasound on liver lipid content of rats

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