Infrared study of phospholipid hydration. Main phase transition of saturated phosphatidylcholine/water multilamellar samples

Mellier, André; Diaf, Abdallah

doi:10.1016/0009-3084(88)90113-2

Cited by 20 publications

(8 citation statements)

References 9 publications

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“…There is ample evidence in the literature demonstrating that such shifts occur when the phosphate-water hydrogen bonds are weakened and the number of water molecules hydrogen-bonded to the phosphate group is decreased. This has been shown for phosphatidylcholine (Arrondo et al, 1984;Mellier & Diaf, 1988), PtdEtn (Sen et al, 1988) and phosphatidylserine (Dluhy et al, 1983;Casal et al, 1984Casal et al, , 1989. No shift of the asymmetric P02-band occurred during the gel-fluid transition, at either pH 9.5 or pH 5.0, but a shift was clearly observed associated to the lamellarhexagonal phase transition.…”

Section: Discussionsupporting

confidence: 52%

Partial dehydration of phosphatidylethanolamine phosphate groups during hexagonal phase formation, as seen by i.r. spectroscopy

Castresana

Nieva

Rivas

et al. 1992

Biochemical Journal

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The gel-to-fluid and lamellar-to-Hii-hexagonal thermotropic phase transitions of egg-yolk phosphatidylethanolamine have been examined by Fourier-transform infrared spectroscopy under a variety of conditions, namely excess water at pH 5.0, excess water at pH 9.5 and low hydration. The various lamellar and hexagonal phases have been characterized by X-ray diffraction. At pH 5.0, gel-fluid and lamellar-hexagonal transitions were detected at 10 and 32°C respectively, in accordance with previous data. At pH 9.5, only the first of these two transitions was detected. In the partially hydrated sample a single phenomenon was observed, probably encompassing both transitions, so that, in practice, a gel-H11-hexagonal transition appears to occur. The region of the i.r. spectrum corresponding to the phospholipid phosphate group reveals that the lamellar-hexagonal, but not the gel-fluid, transition is accompanied by a weakening in the shell of hydrogen-bonded water, thus providing direct evidence that, in a pure lipid/water system, hexagonal phase formation requires partial dehydration of the phospholipid phosphate group. X-ray diffraction data support this conclusion, since, at least in the low-hydration system, the average surface area per lipid polar group decreases with the thermotropic lamellar-hexagonal transition.

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Section: Discussionsupporting

confidence: 52%

Partial dehydration of phosphatidylethanolamine phosphate groups during hexagonal phase formation, as seen by i.r. spectroscopy

Castresana

Nieva

Rivas

et al. 1992

Biochemical Journal

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“…1. In DPPC both pre and main transition T m are clearly observed [11,22] at 36 • C and 42 • C. The addition of a high hexanol concentration results in a broadening and in a shifting to the lower temperature of 30 • C of the main transition, whereas the pre transition appears to be suppressed. These results confirm the disordering effect of alcohol on the lipid hydrocarbon chains and are in agreement with literature data on DPPC [9], and the data on DMPC [19] at lower alcohol concentration.…”

Section: Resultsmentioning

confidence: 96%

Interaction of alcohol with phospholipid membrane: NMR and XRD investigations on DPPC–hexanol system

et al. 2010

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The investigations of the interaction between phospholipid bilayer and short-chain alcohols are relevant for the potential of lipid bilayer membranes to serve as model systems for studies of various biological processes including permeability of the plasma membrane and molecular mechanisms of anesthesia. Because the hydrophobic portion of an alcohol favorably interacts with lipid hydrocarbon chains, the polar hydroxyl group remains free to form hydrogen bonds with polar lipid atoms that are located near the water/lipid interface. Experiments on phospholipid membranes have shown that alcohols can induce an interdigitated phase and at high concentration even promote the assembly of some lipids into non-bilayer structures within the membrane interior. In this paper we have investigated the DPPC:hexanol system at high alcohol concentration (two molecules per phospholipid) by means of calorimetric, Nuclear Magnetic Resonance, X-ray diffraction and density measurements. We have found that the presence of a high alcohol concentration shifts the membrane transition temperature to lower values, and has a disordering effect on the phospholipid acyl chains in the gel phase. The bilayer spacing and the area of polar head have been also derived for the liquid phase.

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“…The stretching vibrations of the NH3' groups are the main part of the broad band complex in the region 3,350-2,250 cm-'. The CH2 groups are fixed in the trans position because their b(CH2) vibration is observed as a sharp band at 1,467 cm-1 (21,22).…”

Section: Resultsmentioning

confidence: 99%

An intramolecular hydrogen bond with large proton polarizability within the head group of phosphatidylserine. An infrared investigation

Leberle¹,

Kempf²,

Zuńdel³

1989

Biophysical Journal

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Films of O-phospho-L-serine-P-ethylester (PSE) were studied by infrared spectroscopy. PSE films were studied pure and as 1:1 mixture with LiOH, NaOH, KOH, and Ca(OH)2 as a function of the degree of hydration. The same investigations were performed if (L-glu)n was added to the system (ratio 1:1, PSE/glu residue). In the PSE molecules an intramolecular (I) COOH...-OP in equilibrium with COO-...HOP (II) hydrogen bond is present. In this bond a double minimum proton potential occurs and it shows large proton polarizability. This hydrogen bond is relatively stable as shown by the neutralization experiments. At low degree of hydration the cations are present at the phosphate groups. The Li ions polarize the intramolecular hydrogen bonds much more than the other cations, i.e., the weight of the proton-limiting structure COOH...-OP is increased by Li ions. Regarding these results one has to assume that such a hydrogen bond is also present in the phosphatidylserine head groups. It is discussed that such hydrogen bonds could be part of a lateral charge-conducting system in the polar surfaces of biological membranes. Such systems could connect proton-creating and proton-consuming centers at the membrane surface and conduct positive charge at an extremely high rate.

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Infrared study of phospholipid hydration. Main phase transition of saturated phosphatidylcholine/water multilamellar samples

Cited by 20 publications

References 9 publications

Partial dehydration of phosphatidylethanolamine phosphate groups during hexagonal phase formation, as seen by i.r. spectroscopy

Partial dehydration of phosphatidylethanolamine phosphate groups during hexagonal phase formation, as seen by i.r. spectroscopy

Interaction of alcohol with phospholipid membrane: NMR and XRD investigations on DPPC–hexanol system

An intramolecular hydrogen bond with large proton polarizability within the head group of phosphatidylserine. An infrared investigation

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