Infrared Studies of Tissue Lipides

Total Lipids and Phospholipids in Turkey Tissues

“…The infrared analysis of the fraction showed a strong band at 6.1 to 6.3 u (Schwarz et al, 1957 and, which is comparable to that of sphingomyelin.…”

“…Chromatographic fraction ations can be followed and the principal components readily determined from the spectra. Accuracy is influenced by variations in the extinction coefficients of various leci thins, etc., and by the composition of the fractions examined, but is probably equal to that attained by microchemical analysis (Nelson and Freeman, 1959;Schwarz et al, 1957)* Only comparatively small samples are necessary for infrared spectrophotometry and they may be recovered if desired. Isolation and quantitative analysis of constitu ents occurring in minor amounts, such as lysophospholipids, ino s itol-containing phospholipids and plasmalogens, would require both a more elaborate fractionation procedure and highly purified reference compounds.…”

Infrared and Raman Microscopy and Imaging of Biomaterials

“…[4][5][6][7][8][9][10][11][12] Variations in the environment of molecular components of materials and tissues are reflected in shifts in absorbance band intensities and positions in vibrational spectra. [4][5][6][7][8][9][10][11][12] Variations in the environment of molecular components of materials and tissues are reflected in shifts in absorbance band intensities and positions in vibrational spectra.…”

Total lipids and phospholipids of turkey tissues

“…The infrared analysis of the fraction showed a strong band at 6.1 to 6.3 u (Schwarz et al, 1957 and, which is comparable to that of sphingomyelin. This spectrum actually represents sphingomyelin with some admixture of lecithins and lysolecithin.…”

“…Chromatographic fraction ations can be followed and the principal components readily determined from the spectra. Accuracy is influenced by variations in the extinction coefficients of various leci thins, etc., and by the composition of the fractions examined, but is probably equal to that attained by microchemical analysis (Nelson and Freeman, 1959;Schwarz et al, 1957)* Only comparatively small samples are necessary for infrared spectrophotometry and they may be recovered if desired. Isolation and quantitative analysis of constitu ents occurring in minor amounts, such as lysophospholipids, ino s itol-containing phospholipids and plasmalogens, would require both a more elaborate fractionation procedure and highly purified reference compounds.…”