Infrared spectroscopy of human cells and tissue. VIII. Strategies for analysis of infrared tissue mapping data and applications to liver tissue

Abstract

Experimental and computational methods of infrared microspectroscopy (IRI‐MSP) and infrared spectral mapping (ISM) are presented. These methods are subsequently applied to the analysis of cirrhotic liver tissue. The sensitivity of infrared spectral mapping toward spectral changes caused by disease will be demonstrated. In addition, the excellent agreement between ISM data and histopathological information will be discussed. © 2000 John Wiley & Sons, Inc. Biopolymers (Biospectroscopy) 57: 282–290, 2000

Fixation protocols for subcellular imaging by synchrotron‐based Fourier transform infrared microspectroscopy

“…13 Hence, we observe intense localization of amide II within the cytoplasm (Figure 2c), which suggests that it is associated with synthesized proteins. The correlation of the amide II vibrational mode with synthesized proteins has also been made by Diem et al 14 It must be noted at this juncture that the intensity distribution of the amide II peak area, presented in Figure 2c and other amide II images of the cells reported in this study, were processed after baseline correction between the spectral region 1578 and 1482 cm Ϫ1 and not between 1730 and 950 cm Ϫ1 as for other peaks (amide I and phosphate) shown in Figure 2g. We find that the intensity distribution of the peak area of the amide II band as a result of baseline correction between 1730 and 950 cm Ϫ1 yielded a similar localization to that observed for the amide I peak area.…”

A Three-Dimensional Multivariate Image Processing Technique for the Analysis of FTIR Spectroscopic Images of Multiple Tissue Sections

“…"horizontal" slices through the hypercube, which displays intensity values at a given wavenumber for all spectral vectors as a false color representation [14]. The intensity value of each spectrum is assigned a color code and displayed against the X and Y coordinates of the spectral element.…”

Fourier transform infrared (FTIR) spectral mapping of the cervical transformation zone, and dysplastic squamous epithelium

“…In this study, we employ an unsupervised clustering approach to investigate cervical tissue. The unsupervised clustering approach is well described in the literature [43][44][45] and only a brief overview is given below.…”