Infrared spectroscopy of exfoliated human cervical cells: evidence of extensive structural changes during carcinogenesis.

Wong, P. T. T.; Wong, Rita K.; Caputo, Thomas A.; Godwin, Thomas; Rigas, Basil

doi:10.1073/pnas.88.24.10988

Cited by 332 publications

(219 citation statements)

References 12 publications

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“…This is in agreement with earlier studies [26,27,30] on exfoliated cervical cells, which correlated abnormality with an observed increase in the intensity of phosphodiester vibrations. In general, these bands appear more intense than the methyl and methylene deformation modes (1450-1350 cm −1 ) in areas of high-grade dysplasia in all samples investigated in this study.…”

Section: Ectocervical Epitheliumsupporting

confidence: 93%

“…Several comprehensive books and articles outline the field [22][23][24][25]. One area that has received considerable attention was the application of the FTIR technique to the diagnosis of cervical neoplasia [26][27][28][29][30][31][32][33][34][35]. Early studies reported spectral differences between exfoliated cells from patients diagnosed normal and dysplastic by cytological [26,27] and histological methods [28,30].…”

Section: The Infrared Alternativementioning

confidence: 99%

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Fourier transform infrared (FTIR) spectral mapping of the cervical transformation zone, and dysplastic squamous epithelium

Wood

Chiriboga

Yee

et al. 2004

Gynecologic Oncology

209

164

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Objective-This paper is aimed at establishing infrared spectral patterns for the different tissue types found in, and for different stages of disease of squamous cervical epithelium. Methods for the unsupervised distinction of these tissue types are discussed.Methods-Fourier transform infrared (FTIR) maps of the squamous and glandular cervical epithelium, and of the cervical transformation zone, were obtained and analyzed by multivariate unsupervised hierarchical cluster methods. The resulting clusters are correlated to the corresponding stained histopathological features in the tissue sections.Results-Multivariate statistical analysis of FTIR spectra collected for tissue sections permit an unsupervised method of distinguishing tissue types, and of differentiating between normal and diseased tissue. By analyzing different spectral windows and comparing the results with histology, we found the amide I and II region (1740-1470 cm −1 ) to be very important in correlating anatomical and histopathological features in tissue to spectral clusters. Since an unsupervised, rather than a diagnostic, algorithm was used in these efforts, no statistical analysis of false-positive/false-negative results is reported at this time.Conclusions-The combination of FTIR micro-spectroscopy and multivariate spectral processing provides important insights into the fundamental spectral signatures of individual cells and consequently shows potential as a diagnostic tool for cervical cancer.

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Section: Ectocervical Epitheliumsupporting

confidence: 93%

Section: The Infrared Alternativementioning

confidence: 99%

Fourier transform infrared (FTIR) spectral mapping of the cervical transformation zone, and dysplastic squamous epithelium

Wood

Chiriboga

Yee

et al. 2004

Gynecologic Oncology

209

164

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“…The changes in the intensity of the shoulder around 1465 cm −1 are more pronounced in the imatinib-resistant K562 cells upon ponatinib introduction. The bands at 1239 and 1086 cm −1 are of nucleic acids and attributed to PO 2 − asymmetric and symmetric stretch [26,27].…”

Section: Resultsmentioning

confidence: 99%

A molecular and biophysical comparison of macromolecular changes in imatinib-sensitive and imatinib-resistant K562 cells exposed to ponatinib

et al. 2015

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Chronic myeloid leukemia (CML) is a type of hematological malignancy that is characterized by the generation of Philadelphia chromosome encoding BCR/ABL oncoprotein. Tyrosine kinase inhibitors (TKIs), imatinib, nilotinib, and dasatinib, are used for the frontline therapy of CML. Development of resistance against these TKIs in the patients bearing T315I mutation is a major obstacle in CML therapy. Ponatinib, the third-generation TKI, is novel drug that is effective even in CML patients with T315I mutation. The exact mechanism of ponatinib in CML has been still unknown. In this study, we aimed to determine the potential mechanisms and structural metabolic changes activated by ponatinib treatment in imatinib-sensitive K562 human CML cell lines and 3 μM-imatinib-resistant K562/IMA3 CML cell lines generated at our lab. Apoptotic and antiproliferative effects of ponatinib on imatinib-sensitive and 3 μM-imatinibresistant K562/IMA3 CML cells were determined by proliferation and apoptosis assays. Additionally, the effects of ponatinib on macromolecules and lipid profiles were also analyzed using Fourier transform infrared spectroscopy (FTIR). Our results revealed that ponatinib inhibited cell proliferation and induced apoptosis as determined by loss of mitochondrial membrane potential, increased caspase-3 enzyme activity, and transfer of phosphatidylserine to the plasma membrane in both K562 and K562/IMA-3 cells. Furthermore, cell cycle analyses revealed that ponatinib arrested K562 and K562/IMA-3 cells at G1 phase. Moreover, ponatinib treatment created a more ordered nucleic acid structure in the resistant cells. Although the lipid to protein ratio increased in imatinib-sensitive K562 cells with a little decrease in the K562/IMA-3 cells, ponatinib treatment indicated significant changes in the lipid composition such as a significant increase in the cellular cholesterol amounts much more in the K562/IMA-3 cells than the sensitive counterparts. Unsaturation in lipids was higher in the resistant cells; however, increases in lipids without phosphate and the number of acyl chains were much higher in the K562 cells. Taken together, all these results showed powerful antiproliferative and apoptotic effects of ponatinib in both imatinib-sensitive and imatinib-resistant CML cells in a dose-dependent manner, and hence, the use of ponatinib for the treatment of TKI-resistant CML patients may be an effective treatment approach in the clinic. More importantly, these results showed that FTIR spectroscopy can detect druginduced physiological changes in cancer drug resistance.

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“…Apart from the above mentioned bands, there are some of those present in the spectrum of the pure enzyme, such as the two signals at 1450 cm -1 and 1490 cm -1 assigned to the scissor vibrations of ≡C-H groups and a signal at 2920 cm -1 generated by the C-H symmetric stretching vibrations of -CH 2 and -CH 3 groups. Also, the most important and characteristic for the pure enzymes are the amide I and amide II bands seen at 1645 cm -1 and 1542 cm -1 respectively [32,33]. Afer the immobilization, those bands were not only noticed to have their intensities decreased, but also their wave numbers (cm -1 ) slightly shifted to 1637 and 1536 cm -1 , respectively.…”

Section: Evaluation Of the Hydrolytic Activity Ofmentioning

confidence: 99%

Immobilization of Amano Lipase A onto Stöber silica surface: process characterization and kinetic studies

Zdarta

Sałek

Kołodziejczak-Radzimska

et al. 2014

Open Chemistry

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The immobilization of Amano Lipase A fromAspergillus niger by adsorption onto Stöber silica matrix obtained by sol-gel method was studied. The effectiveness of the enzyme immobilization and thus the usefulness of the method was demonstrated by a number of physicochemical analysis techniques including Fourier Transform Infrared Spectroscopy (FT-IR), elemental analysis (EA), thermogravimetric analysis (TG), porous structure of the support and the products after immobilization from the enzyme solution with various concentration at different times. The analysis of the process' kinetics allowed the determination of the sorption parameters of the support and optimization of the process. The optimum initial concentration of the enzyme solution was found to be 5 mg mL -1 , while the optimum time of the immobilization was 120 minutes. These values of the variable parameters of the process were obtained by as ensuring the immobilization of the largest possible amount of the biocatalyst at the most economically beneficial aspects of the process.

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Infrared spectroscopy of exfoliated human cervical cells: evidence of extensive structural changes during carcinogenesis.

Abstract: AJISTRACTInfrared spectra were obtained from exfoliated cervical cells from 156 females, of whom 136 were normal, 12-had cervical cancer, and 8 had dysplasia.

Cited by 332 publications

References 12 publications

Fourier transform infrared (FTIR) spectral mapping of the cervical transformation zone, and dysplastic squamous epithelium

Fourier transform infrared (FTIR) spectral mapping of the cervical transformation zone, and dysplastic squamous epithelium

A molecular and biophysical comparison of macromolecular changes in imatinib-sensitive and imatinib-resistant K562 cells exposed to ponatinib

Immobilization of Amano Lipase A onto Stöber silica surface: process characterization and kinetic studies

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