Infrared Spectroscopy of Blood Serum from Patients with Multiple Myeloma

Plotnikova, L.V.; Kobeleva, M. O.; Borisov, E. N.; Гарифуллин, А Д; Povolotckaia, Anastasia; Voloshin, S.; Polyanichko, A. M.

doi:10.1134/s1990519x19020093

Cited by 12 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This change of the system is caused by the fact that a change in conformation (secondary structure) of BS albumins and immunoglobulins takes place in patients [24,25]. The later circumstance, as shown above, can result in partial dimerization of serum proteins [26,27]. As a consequence, dipole moments of proteins and their dimers change towards their increase.…”

Section: G(τ)mentioning

confidence: 97%

“…The detected features of donor and patient DS, that is the conductivity decrease in the region of high frequencies, as well as the correlation between the power exponent s and the total protein content, and changes in the spectrum of relaxation complexes responsible for relaxation in the region of low frequencies indicate the change in quantitative relationship between blood components in the case of disease. This change of the system is caused by the fact that a change in conformation (secondary structure) of BS albumins and immunoglobulins takes place in patients [24,25]. The later circumstance, as shown above, can result in partial dimerization of serum proteins [26,27].…”

Section: G(τ)mentioning

confidence: 97%

See 1 more Smart Citation

Dielectric study of blood serum of patients with oncohematological diseases

Kastro

Valerievna

et al. 2022

Optics and Spectroscopy

View full text Add to dashboard Cite

Features of charge transfer and dielectric relaxation processes in blood serum samples of patients with oncohematological diseases and healthy donors were investigated by dielectric spectroscopy method. The observed features of the dielectric spectra for donors and patients, namely the decrease in conductivity in the high frequency region, the correlation between the degree indicator s and the total protein content, as well as changes in the spectrum of relaxation complexes indicate a change in the quantitative ratios of blood components in the presence of disease. This system rearrangement is the result of the fact that conformation of proteins (albumins and immunoglobulins) for patients is change. Keywords: Dielectric spectroscopy, serum, chronic lymphocytic leukemia, multiple myeloma, charge transfer, dielectric relaxation.

show abstract

Section: G(τ)mentioning

confidence: 97%

Section: G(τ)mentioning

confidence: 97%

Dielectric study of blood serum of patients with oncohematological diseases

Kastro

Valerievna

et al. 2022

Optics and Spectroscopy

View full text Add to dashboard Cite

show abstract

“…Первичную обработку и анализ спектров проводили с помощью программного обеспечения, поставляемого с прибором, в соответствии с описанной ранее методикой [22,23]. Как уже отмечалось выше, форма полосы Амид I весьма чувствительна ко вторичной структуре белков [20]. В связи с тем, что развитие ММ сопровождается существенным изменением белкового состава сыворотки крови [8][9][10], анализ полосы Амид I позволяет выявлять статистически значимые различия в средних значениях основных параметров вторичной структуры белков в составе сыворотки пациентов с диагнозом ММ и здоровых доноров [18,28].…”

Section: ик спектроскопияunclassified

“…В связи с этим возникает необходимость в поиске новых простых и доступных методик первичного скрининга широкого круга пациентов с целью выявления признаков развития ММ. Одним из перспективных инструментов для выявления различных патологий может выступать инфракрасная (ИК) спектроскопия [15][16][17][18][19][20]. В настоящей работе проведен сравнительный анализ ИК спектров сыворотки крови больных множественной миеломой и здоровых доноров.…”

Section: Introductionunclassified

Особенности Инфракрасных Спектров Сыворотки Крови Больных Множественной Миеломой

Чернышев,

Михайлец,

Плотникова

et al. 2023

Оптика И Спектроскопия

View full text Add to dashboard Cite

Comparative analysis of infrared (IR) spectra of blood serum of healthy donors and patients with multiple myeloma (MM) was performed. MM is characterized by hyperproduction of the paraprotein, which manifests itself in a change in the spectral pattern of mid-IR absorption spectra. It has been shown that the most significant deviations in the spectra were observed for samples of MM near the Amide I and Amide II´ bands. As a result of a comparative analysis of the IR spectra of blood serum in the region of 1700-1350 cm-1, nine parameters were established, the average values of which in the samples obtained from patients with a secretory form of MM differ significantly from similar indicators for the serum of healthy donors.

show abstract

“…During last years, Fourier Transform Infrared (FTIR) micro-spectroscopy has been widely used to investigate different types of biomedical samples, as tissues [1][2][3], biological fluids [4][5][6], and cells [6][7][8][9]. One of the main biomedical applications of FTIR micro-spectroscopy concerns the possibility to discriminate cancer cells from their normal counterpart according to specific spectral changes.…”

Section: Introductionmentioning

confidence: 99%

A Comparison between FTIR Spectra from HUKE and SH-SY5Y Cell Lines Grown on Different Substrates

2020

View full text Add to dashboard Cite

In recent years, Fourier Transform Infrared (FTIR) micro-spectroscopy has shown promising potential in medical diagnostics at the cellular level. In fact, FTIR spectra can provide information related to DNA, protein, and lipid content and how such a content changes when a pathological state arises. Most of these information is included in the so-called fingerprint region (1000–1800 cm−1), consisting of several spectral peaks related to vibrational modes occurring inside cellular components. Unfortunately, the slides commonly used in cytology (as the glass microscopy slides and coverslips) are opaque to IR radiation in the fingerprint region, whereas they are transparent for wavenumber values larger than 2000 cm−1, where few and broad spectral absorption bands, mainly due to lipids and proteins, are present. Nonetheless, here we show that FTIR spectra performed in the high wavenumber range 2750–3000 cm−1 can be used to discriminate two different types of cells, one from a normal cell line (Human Keratinocyte, HUKE) and the other from a cancer one (SH-SY5Y). The spectra are discriminated by means of their Principal Component Analysis, according to the PC1 component, and by means of ratiometric analysis, according to the ratio of the intensity of the peak at 2956 cm−1 and that of the peak at 2924 cm−1. The PC1 score values of the HUKE are statistically different from the PC1 score values of SH-SY5Y, whereas the intensity ratio results larger for SH-SY5Y than for HUKE cells. Such results occur for different substrates over which the cells have been grown, including the thick glass slides used for cytological analysis. This result is a further step toward the application of FTIR microspectroscopy in the cytological routine diagnosis.

show abstract

Infrared Spectroscopy of Blood Serum from Patients with Multiple Myeloma

Cited by 12 publications

References 11 publications

Dielectric study of blood serum of patients with oncohematological diseases

Dielectric study of blood serum of patients with oncohematological diseases

Особенности Инфракрасных Спектров Сыворотки Крови Больных Множественной Миеломой

A Comparison between FTIR Spectra from HUKE and SH-SY5Y Cell Lines Grown on Different Substrates

Contact Info

Product

Resources

About