Infrared spectroscopic investigation of erythrocyte membrane-smoke interactions due to chronic cigarette smoking

Mahmoud, Sherif S.; Mervat, Ali A; Aly, Eman Mohamed

doi:10.4149/gpb_2016047

Cited by 3 publications

(1 citation statement)

References 24 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is mainly being used for the changes in the secondary structures of proteins and polypeptides. Hence, the reduction in the α-helix and elevation in the β-turn contents associated with amiodarone indicate that the drug administration changed the solubility and folding of the optic nerve protein [37].…”

Section: Discussionmentioning

confidence: 99%

Effect of Amiodarone on Rabbits' Optic Nerve and the Ameliorative Effect of Vitamin E: Ftir Study

et al. 2022

View full text Add to dashboard Cite

Objective: The study aimed to investigate the structural and conformational changes induced by short-term administration of the amiodarone in the optic nerve besides validating whether vitamin E coadministration with amiodarone will improve these changes. Methods: Thirty New Zealand white rabbits from both sexes were haphazardly categorized into three groups, whereas each group contains ten rabbits (20 eyes). One of these groups served as a control that received an intraperitoneal injection of normal saline. Rabbits in the second group intraperitoneally (ip) injected daily with 160 mg/kg body weight (bw) of amiodarone for two weeks. The last group orally administration 100 mg/kg bw of vitamin E with the 160 mg/kg bw of amiodarone ip daily for two weeks until the time of sacrifice. Fourier transform infrared spectroscopy (FTIR) analysis was conducted on the optic nerve of the all groups. Results: The results obtained from the FTIR spectrum revealed that the short-term administration of amiodarone caused a significant alteration in the stretching NH-OH region. A newly detected component centered at 3739±1 cm-1 was assigned as strO-H. There was a significant decrease (p˂0.05) in the bandwidth and band position of one component of strO-H that centered at 3598±1 cm-1. Moreover, remaining vibrational bands (O-Hasym and O-Hsym) were shifted to higher frequencies. Coadministration of vitamin E with amiodarone reduced the contour to four components as a control with significant increase in the band position of O-Hasym and the bandwidth of one component of str O-H. Amiodarone administrations lead to reducing the area ratio of asymCH2 to symCH2 and elevation of the area ratio of asymCH2 to asymCH3 while the coadministration of vitamin E returned it as the control ratio. The percentage of the β-turn was significantly increased while the α-helix content was decreased due to amiodarone. The contents of both components were considered mimicking the control values when Vitamin E was co-administered with amiodarone. Conclusion: The study stated that amiodarone could change the solubility and folding of the optic nerve proteins. Finally, vitamin E intake with amiodarone turns many of these changes induced by amiodarone to normal levels, which make it a good supplement for amiodarone users.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of Amiodarone on Rabbits' Optic Nerve and the Ameliorative Effect of Vitamin E: Ftir Study

et al. 2022

View full text Add to dashboard Cite

show abstract

Side Effects of Chemotherapy on the Molecular Structure of rat’s retina and the Possible Protective Role of Antioxidants

Fouad

Kamal

Awad

2020

Journal of Scientific Research in Science

View full text Add to dashboard Cite

Ifosfamide is an alkylating oxazaphosphorine antitumor prodrug. Although it is an effective chemotherapeutic agent, it has been shown to induce many side effects. The objective of the present study, therefore, was to investigate the possible protective roles of lecithin and quercetin either singly or combined against ifosfamide-induced molecular structure changes in retina of female rats using Fourier transform infrared spectroscopy (FTIR). Seventy female albino rats were randomly divided into seven groups. Ifosfamide (Ifo; 80mg/kg b.wt.) was administrated for five consecutive days intraperitoneally (i.p.), while quercetin (50mg/kg b.wt.) and lecithin (100mg/kg b.wt) were given orally either singly or in-combination with IFO for six consecutive days. Our results indicate that Ifosfamide was affected on the lipid components of the retina, NH-OH region changes revealed unusual interface/binding mechanism that related to different surrounding environment due to ifosfamide intraperitoneal injection. The co-administration of Lec+Que with the intraperitoneal injection of Ifosfamide preventing the side effect of Ifosfamide. We suggest that synergistic effect of quercetin and lecithin in the combined therapy results in marked neuroprotective effect in part through its antioxidant properties and down regulation of molecular structure in retina.

show abstract

Using Fourier Transform Infrared (FTIR) Spectroscopy Coupled with Multivariate Analysis to Diagnose Polycystic Ovary Syndrome (PCOS)

2020

SRP

View full text Add to dashboard Cite

Infrared spectroscopic investigation of erythrocyte membrane-smoke interactions due to chronic cigarette smoking

Cited by 3 publications

References 24 publications

Effect of Amiodarone on Rabbits' Optic Nerve and the Ameliorative Effect of Vitamin E: Ftir Study

Effect of Amiodarone on Rabbits' Optic Nerve and the Ameliorative Effect of Vitamin E: Ftir Study

Side Effects of Chemotherapy on the Molecular Structure of rat’s retina and the Possible Protective Role of Antioxidants

Using Fourier Transform Infrared (FTIR) Spectroscopy Coupled with Multivariate Analysis to Diagnose Polycystic Ovary Syndrome (PCOS)

Contact Info

Product

Resources

About