Infrared Spectra of Steroids

Levchuk, Yu. N.

doi:10.1070/rc1968v037n02abeh001621

Cited by 7 publications

(10 citation statements)

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“…Additional PS vibrational peaks were observed at $980 cm -1 (CQC trans), $1590, and $1650 cm À1 (CQC stretching) [35], and in the range 3200-3400 cm À1 (OH intermolecular H bonds). The hydroxyl group in PS molecules comprised two partially overlapped, wide peaks at 3300 and 3424 cm À1 , similar to the two overlapping peaks that were observed by Lechuk [33] for ergosterol crystals. These vibrations were assigned to the double bond of the stigmasterol carbon chain that is one of the major components of the PS commercial mixture.…”

Section: Effect On Crystal Structurementioning

confidence: 53%

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Templated cocrystallization of cholesterol and phytosterols from microemulsions

Rozner

Popov

Uvarov

et al. 2009

Journal of Crystal Growth

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Section: Effect On Crystal Structurementioning

confidence: 53%

“…The sterols exhibited characteristic vibration bands ( Fig. 2) between 2860 and 2930 cm À1 [32][33][34]. The C-OH stretching vibration for CH and PS in sterols derived from the OH group at the 3b-position and at a 5D-double bond were observed at $1050 cm -1 .…”

Section: Effect On Crystal Structurementioning

confidence: 97%

Templated cocrystallization of cholesterol and phytosterols from microemulsions

Rozner

Popov

Uvarov

et al. 2009

Journal of Crystal Growth

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“…3b , red lines) displayed a higher intensity of the ~1374 cm −1 and of the ~1238 cm −1 bands. In addition, the simultaneous increase in intensity of the ~2936 cm −1 , 2907 cm −1 and 1374 cm −1 absorptions suggests an enrichment in cholesterol 36 43 , in the same tissue areas affected by amyloid deposits ( Fig. 4b ).…”

Section: Resultsmentioning

confidence: 91%

In situ characterization of protein aggregates in human tissues affected by light chain amyloidosis: a FTIR microspectroscopy study

Ami

Lavatelli

Rognoni

et al. 2016

Sci Rep

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Light chain (AL) amyloidosis, caused by deposition of amyloidogenic immunoglobulin light chains (LCs), is the most common systemic form in industrialized countries. Still open questions, and premises for developing targeted therapies, concern the mechanisms of amyloid formation in vivo and the bases of organ targeting and dysfunction. Investigating amyloid material in its natural environment is crucial to obtain new insights on the molecular features of fibrillar deposits at individual level. To this aim, we used Fourier transform infrared (FTIR) microspectroscopy for studying in situ unfixed tissues (heart and subcutaneous abdominal fat) from patients affected by AL amyloidosis. We compared the infrared response of affected tissues with that of ex vivo and in vitro fibrils obtained from the pathogenic LC derived from one patient, as well as with that of non amyloid-affected tissues. We demonstrated that the IR marker band of intermolecular β-sheets, typical of protein aggregates, can be detected in situ in LC amyloid-affected tissues, and that FTIR microspectroscopy allows exploring the inter- and intra-sample heterogeneity. We extended the infrared analysis to the characterization of other biomolecules embedded within the amyloid deposits, finding an IR pattern that discloses a possible role of lipids, collagen and glycosaminoglycans in amyloid deposition in vivo.

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“…In particular, the second derivative spectrum of cells grown in the absence of lactic acid is characterized by a number of well resolved bands mainly due to the CH 2 and CH 3 deformation modes: the ~1467 cm −1 band is due to the overlapping absorption of CH 2 and CH 3 [ 22 , 24 , 33 ], while the ~1455, 1440 and 1368 cm −1 bands are due to CH 3 [ 22 , 24 ], and the ~1414 cm −1 absorption to CH 2 [ 34 ]. In addition, the component at ~1400 cm −1 is mainly assigned to the CH 3 bending vibration of the N(CH 3 ) 3 head group of phosphatidylcholine (PC) and the absorption at ~1387 cm −1 can be assigned to the CH 3 deformation mainly arising from ergosterol [ 22 , 35 , 36 ]. Finally, the component at ~1246 cm −1 is also observed, due to the PO 2 - stretching mode mainly from phospholipids and nucleic acids [ 20 , 22 ].…”

Section: Resultsmentioning

confidence: 99%

“…3 c) the spectrum of cells grown at pH3 is characterized by four well resolved and intense bands due to the CH 2 (at ~2921 and 2851 cm −1 ) and CH 3 (at ~2959 and 2872 cm −1 ) stretching vibrations of lipid hydrocarbon tails [ 22 , 24 ]. A shoulder around 2935 cm −1 is also present, that can be mainly assigned to the CH 2 stretching of ergosterol [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

et al. 2016

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BackgroundLactic acid is a versatile chemical platform with many different industrial applications. Yeasts have been demonstrated as attractive alternative to natural lactic acid producers since they can grow at low pH, allowing the direct purification of the product in the desired acidic form. However, when very high concentrations of organic acids are reached, the major limitation for a viable production is the toxic effect of the product. The accumulation in the cytosol of H+ and of the weak organic counter-anions triggers a cellular reprogramming. Here, the effects of lactic acid exposure on Saccharomycescerevisiae have been evaluated by Fourier transform infrared (FTIR) microspectroscopy. In addition to -omic techniques, describing these responses in terms of systems and networks, FTIR microspectroscopy allows a rapid acquisition of the cellular biochemical fingerprint, providing information on the major classes of macromolecules.ResultsFTIR analyses on Saccharomyces cerevisiae cells under lactic acid stress at low pH revealed some still uncharacterized traits: (1) a direct correlation between lactic acid exposure and a rearrangement in lipid hydrocarbon tails, together with a decrease in the signals of phosphatidylcholine (PC), one of the main components of cell membrane; (2) a rearrangement in the cell wall carbohydrates, including glucans and mannans (3) a significant yet transient protein aggregation, possibly responsible for the observed transient decrease of the growth rate. When repeated on the isogenic strain deleted in OPI1, encoding for a transcriptional repressor of genes involved in PC biosynthesis, FTIR analysis revealed that not only the PC levels were affected but also the cell membrane/wall composition and the accumulation of protein aggregates, resulting in higher growth rate in the presence of the stressing agent.ConclusionsThis work revealed novel effects evoked by lactic acid on cell membrane/wall composition and protein aggregation in S. cerevisiae cells. We consequently demonstrated that the targeted deletion of OPI1 resulted in improved lactic acid tolerance. Considering that stress response involves many and different cellular networks and regulations, most of which are still not implemented in modelling, these findings constitute valuable issues for interpreting cellular rewiring and for tailoring ameliorated cell factories for lactic acid production.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-016-0438-2) contains supplementary material, which is available to authorized users.

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Infrared Spectra of Steroids

Abstract: The resonant elastic scattering of y rays by 144Nd nuclei has been investigated and the small cross section observed indicates a lower limit of 2.0 50.4 x1O-14 seconds for the lifetime of the 2.18 MeV (I-) level.

Cited by 7 publications

References 12 publications

Templated cocrystallization of cholesterol and phytosterols from microemulsions

Templated cocrystallization of cholesterol and phytosterols from microemulsions

In situ characterization of protein aggregates in human tissues affected by light chain amyloidosis: a FTIR microspectroscopy study

Protein aggregation and membrane lipid modifications under lactic acid stress in wild type and OPI1 deleted Saccharomyces cerevisiae strains

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